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 PortabellaFella 1
Enthusiastic
 
Registered: 08/08/16
Posts: 654
Last seen: 3 years, 17 days
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 Re: Spawn clean vs dirty identification pictures  [Re: ComebackKid]
#23689618 - 09/29/16 07:58 AM (7 years, 6 months ago) |
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Ok I've only just begun research into agar, that makes sense. Stones and stuck agar wedges make sense too but no parameters of inoculation or if stone producing sp. was used. I was just assuming it wasn't agar because none of the other pics show the same spot, big or small. Assume bad, fact good. <---- insert cave man voice while reading.
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I would like to acquire anything I don’t have
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 JacobStorm
psychedelic cartel
Registered: 07/21/15
Posts: 1,499
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Re: Spawn clean vs dirty identification pictures [Re: ComebackKid]
#23689669 - 09/29/16 08:13 AM (7 years, 6 months ago) |
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Quote:
ComebackKid said:
Quote:
PortabellaFella 1 said:
That's a huge piece of agar
Most likely from tiger drop method where you pop n drop the whole plate of agar from a pasty plate into a jar of grain
 Sounds intense. Why would you drop the whole plate?
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MadSeasonStudent
Enjoying Life
Registered: 08/26/12
Posts: 758
Loc: Swinging on the spiral
Last seen: 2 months, 23 days
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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
#23689688 - 09/29/16 08:19 AM (7 years, 6 months ago) |
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Sometimes you just want a jar to colonize faster so drop the whole plate inside.
If you have 50 plates it wont matter if you use the whole plate or not.
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Peteyboy
SpaceWalker
Registered: 06/21/16
Posts: 2,848
Loc: Trumperica!
Last seen: 4 years, 8 months
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Re: Spawn clean vs dirty identification pictures [Re: MadSeasonStudent]
#23689700 - 09/29/16 08:25 AM (7 years, 6 months ago) |
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If you have clean myc on agar, I have found it easier/faster to Tiger drop the dish then grain to grain out the original jar, but Im not sure how long the culture will maintain its virility as I've just started doing this.
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JacobStorm
psychedelic cartel
Registered: 07/21/15
Posts: 1,499
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Re: Spawn clean vs dirty identification pictures [Re: Peteyboy]
#23689718 - 09/29/16 08:32 AM (7 years, 6 months ago) |
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Huh. Okay well kick ass  I didnt know that. Wouldn't have occurred to me to drop the whole plate.
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Rogger Rabbits Mushroom growing videos
Ethnobotanical garden forum
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azur said: If you like 6th grade results, then 6th grade projects are great.
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dankington
The Stranger
Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
#23689748 - 09/29/16 08:46 AM (7 years, 6 months ago) |
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I probably have pictures you could use. I'll take a look.
Good idea here, Bodhi!
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spacechildo
proletarians rise up
Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 7 months
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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
#23689753 - 09/29/16 08:48 AM (7 years, 6 months ago) |
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tiger drop is so you dont have to cut your dish at all, just squeeze and drop.
if you want faster colonization you cut the dish into several smaller pieces and drop them all in the jar.
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Boogieman47
Let's boogie
Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Spawn clean vs dirty identification pictures [Re: spacechildo]
#23689763 - 09/29/16 08:52 AM (7 years, 6 months ago) |
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Ya its from a tiger drop and like space said if you cut them into smaller pieces its faster maybe not even have to shake if its fast myc and even dispersed
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PortabellaFella 1
Enthusiastic
Registered: 08/08/16
Posts: 654
Last seen: 3 years, 17 days
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Re: Spawn clean vs dirty identification pictures [Re: Boogieman47]
#23689950 - 09/29/16 10:08 AM (7 years, 6 months ago) |
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Great info here guys thank you
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I would like to acquire anything I don’t have
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JacobStorm
psychedelic cartel
Registered: 07/21/15
Posts: 1,499
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Thats fucking awesome. So is a fully colonized agar plate resistant to contam and bactiriea? Like a fully colonized BRF cake?
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Ethnobotanical garden forum
Inocuole tea TEK
azur said: If you like 6th grade results, then 6th grade projects are great.
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bodhisatta  
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
#23690053 - 09/29/16 10:35 AM (7 years, 6 months ago) |
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No, agar is used as an inoculant so it has to stay clean and sterile.
Cakes are resistant after birthing yes but you don't use them to inoculate something.
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spacechildo
proletarians rise up
Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 7 months
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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
#23690057 - 09/29/16 10:35 AM (7 years, 6 months ago) |
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Quote:
JacobStorm said:
Thats fucking awesome. So is a fully colonized agar plate resistant to contam and bactiriea? Like a fully colonized BRF cake?
eh.. you dont want mold spores on your colonized agar plates, the grains you put said wedge on wont like the contams.
cutting your agar dish is a contam vector, dirty scalpel, poor technique etc, tigerdropping eliminates that vector.
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JacobStorm
psychedelic cartel
Registered: 07/21/15
Posts: 1,499
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Re: Spawn clean vs dirty identification pictures [Re: bodhisatta]
#23690109 - 09/29/16 10:50 AM (7 years, 6 months ago) |
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Quote:
spacechildo said:
Quote:
JacobStorm said:
Thats fucking awesome. So is a fully colonized agar plate resistant to contam and bactiriea? Like a fully colonized BRF cake?
eh.. you dont want mold spores on your colonized agar plates, the grains you put said wedge on wont like the contams.
cutting your agar dish is a contam vector, dirty scalpel, poor technique etc, tigerdropping eliminates that vector.
Right so its not tigerdropping unless we chop it up. This is great!Quote:
bodhisatta said:
No, agar is used as an inoculant so it has to stay clean and sterile.
Cakes are resistant after birthing yes but you don't use them to inoculate something.
Yeah I get that it makes sense.
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Rogger Rabbits Mushroom growing videos
Ethnobotanical garden forum
Inocuole tea TEK
azur said: If you like 6th grade results, then 6th grade projects are great.
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Boogieman47
Let's boogie
Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
#23690116 - 09/29/16 10:53 AM (7 years, 6 months ago) |
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No tiger drop is tge whole piece dropped in tic tac tiger drop is cutting it
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spacechildo
proletarians rise up
Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 7 months
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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
#23690150 - 09/29/16 11:03 AM (7 years, 6 months ago) |
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cutting the agar plate is "normal".
not cutting it is called tiger drop here, after elasticaltiger.
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Boogieman47
Let's boogie
Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Spawn clean vs dirty identification pictures [Re: spacechildo]
#23690177 - 09/29/16 11:11 AM (7 years, 6 months ago) |
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Has anyone heard from him ? Just wondering how hew doing?
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blackdust
Registered: 02/28/09
Posts: 8,327
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Re: Spawn clean vs dirty identification pictures [Re: Boogieman47]
#23703980 - 10/03/16 03:00 PM (7 years, 6 months ago) |
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Fuck
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spacechildo
proletarians rise up
Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 7 months
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Re: Spawn clean vs dirty identification pictures [Re: blackdust]
#23704084 - 10/03/16 03:39 PM (7 years, 6 months ago) |
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RyeJar
StrangerDanger
Registered: 04/16/15 
Posts: 523
Last seen: 1 year, 22 days
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Re: Spawn clean vs dirty identification pictures [Re: spacechildo]
#23704281 - 10/03/16 04:45 PM (7 years, 6 months ago) |
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Amazing idea bod. Great post. Cant wait for it to be finished. Its actually sad we dont have something like this already after all these years.
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ComebackKid
Multispore Enthusiast
Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Spawn clean vs dirty identification pictures [Re: RyeJar]
#23704348 - 10/03/16 05:03 PM (7 years, 6 months ago) |
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Bods been picking up your slack 'round here RyeJar... if that's even your real name
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Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.
Our individual experiences are all part of the universe's experience of itself
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