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InvisiblebodhisattaMDiscordReddit
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Spawn clean vs dirty identification pictures * 4
    #23662280 - 09/20/16 04:18 PM (7 years, 4 months ago)

Hopefully this can become a useful catalog with everyone's help

OATS

Healthy:


After shake and before shake. These jars are filled to the brim and yet the spawn is still easy to break up. You spawn should break up easily if healthy.




After shake recovery(within 48hours):


Roughly 30 hours after a shake at 50% colonization



Bacterial:


Couldn't shake stayed in clumps while others of the same batch broke up easily. No recovery of further colonization after the shake.


supplemental information


Other(totally fucked and molds):




RYE

Healthy:

After shake recovery(within 48hours):

Bacterial:

Other(totally fucked and molds):



WBS

Healthy:

After shake recovery(within 48hours):

Bacterial:

Other(totally fucked and molds):



WHEAT

Healthy:


Courtesy of Enlightenment

After shake recovery(within 48hours):

Bacterial:




Right after shaking:



After 48 hours:


Courtesy of Enlightenment

Other(totally fucked and molds):


to be continued

Healthy:

After shake recovery(within 48hours):

Bacterial:

Other(totally fucked and molds):




I'll continue to update and make it look pretty as more pictures/results come in


Edited by bodhisatta (01/23/17 03:00 PM)


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OfflinePeteyboy
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Re: Spawn clean vs dirty identification pictures [Re: bodhisatta]
    #23662384 - 09/20/16 04:55 PM (7 years, 4 months ago)

Awesome pictorial


:popcorn:


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OfflineTira
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Re: Spawn clean vs dirty identification pictures [Re: Peteyboy]
    #23662492 - 09/20/16 05:45 PM (7 years, 4 months ago)

Been looking for something like this for a while .) Thanks a lot for doing it


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Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


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Re: Spawn clean vs dirty identification pictures [Re: Tira] * 1
    #23662541 - 09/20/16 06:03 PM (7 years, 4 months ago)

awesome idea!!

this and food dehydrators need to be sticky threads!
                  (just plugging my own thread)
https://www.shroomery.org/forums/showflat.php/Number/23652987/page/1

Here are two bacterial jars! still produced fruits. currently on flush 2


here is a bacterial bag of hpoo. spawned to a tub. got half a flush and went green.


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InvisibleJacobStorm
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Re: Spawn clean vs dirty identification pictures [Re: mushboy]
    #23662575 - 09/20/16 06:18 PM (7 years, 4 months ago)

Sweet Bod, this was a cool idea. I sense a TC tag in the future.
Did you purposely ruin jars for the sake of the thread?


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InvisibleComebackKid
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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
    #23662602 - 09/20/16 06:25 PM (7 years, 4 months ago)

First time doing grains and had great spawn that broke up just from the g force of shaking.
I laughed to myself thinking about all the posts I read about people taking their jars to a tire before spawning or bruised hands.

Agar ftw!


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


Edited by ComebackKid (09/20/16 06:37 PM)


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OfflineTira
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Re: Spawn clean vs dirty identification pictures [Re: mushboy]
    #23662626 - 09/20/16 06:30 PM (7 years, 4 months ago)

Some bacterial wheat (smelled really sour, yeast-like when I opened  the jars)

     

It was a bitch to break the grains; my hand was sore the day after. Spawn in jars are currently colonizing a monotub and I am currently working on agar for a better future.


--------------------
Useful Links for Beginners

The Basics              AMU Teks

Frank''s Teks          Agar

Noob Forum Reccomended Teks

Agar for guaranteed spawn,
Proper pasteurization for guaranteed substrate.


Edited by Tira (09/21/16 11:46 AM)


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OfflineHamHead
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Re: Spawn clean vs dirty identification pictures [Re: Tira]
    #23663001 - 09/20/16 08:16 PM (7 years, 4 months ago)



Some bacterial WBS, smelled sweet/sour when spawning. Sat in the tubs for 3 weeks and never did anything.


--------------------
The Italian researchers’ findings, published by the INT’s scientific magazine Tumori Journal, show 11.6% of 959 healthy volunteers enrolled in a lung cancer screening trial between September 2019 and March 2020 had developed coronavirus antibodies well before February.

https://www.reuters.com/article/us-health-coronavirus-italy-timing-idUSKBN27V0KF

This online first version has been peer-reviewed, accepted and edited,  but not formatted and finalized with corrections from authors and proofreaders

https://www.icandecide.org/


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InvisiblebodhisattaMDiscordReddit
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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
    #23663014 - 09/20/16 08:19 PM (7 years, 4 months ago)

Quote:

JacobStorm said:
Did you purposely ruin jars for the sake of the thread?



Nope. I used needles/syringes for the little bit of growing I've gotten around to this year and it has its consequences. Inoculation done open air with syringes I used my gas stove to flame the needles. and the LC was made with the same plate I put clone tissue on so I wouldn't be surprised if that had some to do with losing a couple jars.


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InvisibleChanga Alchemist
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Re: Spawn clean vs dirty identification pictures [Re: bodhisatta]
    #23687239 - 09/28/16 03:39 PM (7 years, 4 months ago)

Very nice. I've also been searching for something like this for some time. Thanks!!


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:ganja::mushroom::mushroom::flyhigh:
:sporedrop:~~~:peyote::sanpedro:~~~:sporedrop:

:chugbeer:


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Re: Spawn clean vs dirty identification pictures [Re: Changa Alchemist]
    #23688865 - 09/28/16 10:54 PM (7 years, 4 months ago)

:popcorn:


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:vaped: Highwalker :bongload:


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InvisibleBoogieman47
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Re: Spawn clean vs dirty identification pictures [Re: lukehighwalker710]
    #23688871 - 09/28/16 10:57 PM (7 years, 4 months ago)

... second pic, 3rd pic, 5th. And 6th are good


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Re: Spawn clean vs dirty identification pictures [Re: Boogieman47]
    #23689372 - 09/29/16 06:16 AM (7 years, 3 months ago)

5th pic is good with that giant yellow loogie tumor? Metabolites just digesting?


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Re: Spawn clean vs dirty identification pictures [Re: PortabellaFella 1]
    #23689378 - 09/29/16 06:20 AM (7 years, 3 months ago)

That just looks like agar stripped of its color...


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OfflinePortabellaFella 1
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Re: Spawn clean vs dirty identification pictures [Re: Peteyboy]
    #23689395 - 09/29/16 06:31 AM (7 years, 3 months ago)

That's a huge piece of agar


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Re: Spawn clean vs dirty identification pictures [Re: PortabellaFella 1]
    #23689457 - 09/29/16 07:05 AM (7 years, 3 months ago)

Yeah I thought the same thing but maybe a couple pieces stuck together, it happens sometimes in my jars....


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InvisibleJacobStorm
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Re: Spawn clean vs dirty identification pictures [Re: Peteyboy]
    #23689502 - 09/29/16 07:25 AM (7 years, 3 months ago)

Yeah my guess/vote is its a stone.


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Ethnobotanical garden forum

Inocuole tea TEK

azur said: If you like 6th grade results, then 6th grade projects are great.


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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
    #23689517 - 09/29/16 07:29 AM (7 years, 3 months ago)

Added to the list of visuals marked as "ok"


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Re: Spawn clean vs dirty identification pictures [Re: PortabellaFella 1]
    #23689536 - 09/29/16 07:34 AM (7 years, 3 months ago)

On closer inspection it looks like 3 pieces of agar clumped together...


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InvisibleComebackKid
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Re: Spawn clean vs dirty identification pictures [Re: PortabellaFella 1]
    #23689546 - 09/29/16 07:37 AM (7 years, 3 months ago)

Quote:

PortabellaFella 1 said:
That's a huge piece of agar




Most likely from tiger drop method where you pop n drop the whole plate of agar from a pasty plate into a jar of grain


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflinePortabellaFella 1
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Re: Spawn clean vs dirty identification pictures [Re: ComebackKid]
    #23689618 - 09/29/16 07:58 AM (7 years, 3 months ago)

Ok I've only just begun research into agar, that makes sense. Stones and stuck agar wedges make sense too but no parameters of inoculation or if stone producing sp. was used. I was just assuming it wasn't agar because none of the other pics show the same spot, big or small. Assume bad, fact good. <---- insert cave man voice while reading.


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InvisibleJacobStorm
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Re: Spawn clean vs dirty identification pictures [Re: ComebackKid]
    #23689669 - 09/29/16 08:13 AM (7 years, 3 months ago)

Quote:

ComebackKid said:
Quote:

PortabellaFella 1 said:
That's a huge piece of agar




Most likely from tiger drop method where you pop n drop the whole plate of agar from a pasty plate into a jar of grain




:what: Sounds intense. Why would you drop the whole plate?


--------------------
Rogger Rabbits Mushroom growing videos

Ethnobotanical garden forum

Inocuole tea TEK

azur said: If you like 6th grade results, then 6th grade projects are great.


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OfflineMadSeasonStudent
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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
    #23689688 - 09/29/16 08:19 AM (7 years, 3 months ago)

Sometimes you just want a jar to colonize faster so drop the whole plate inside.
If you have 50 plates it wont matter if you use the whole plate or not.


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Re: Spawn clean vs dirty identification pictures [Re: MadSeasonStudent]
    #23689700 - 09/29/16 08:25 AM (7 years, 3 months ago)

If you have clean myc on agar, I have found it easier/faster to Tiger drop the dish then grain to grain out the original jar, but Im not sure how long the culture will maintain its virility as I've just started doing this.


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InvisibleJacobStorm
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Re: Spawn clean vs dirty identification pictures [Re: Peteyboy]
    #23689718 - 09/29/16 08:32 AM (7 years, 3 months ago)

Huh. Okay well kick ass :thumbup: I didnt know that. Wouldn't have occurred to me to drop the whole plate.


--------------------
Rogger Rabbits Mushroom growing videos

Ethnobotanical garden forum

Inocuole tea TEK

azur said: If you like 6th grade results, then 6th grade projects are great.


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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
    #23689748 - 09/29/16 08:46 AM (7 years, 3 months ago)

I probably have pictures you could use. I'll take a look.

Good idea here, Bodhi!


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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
    #23689753 - 09/29/16 08:48 AM (7 years, 3 months ago)

tiger drop is so you dont have to cut your dish at all, just squeeze and drop.
if you want faster colonization you cut the dish into several smaller pieces and drop them all in the jar.


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Re: Spawn clean vs dirty identification pictures [Re: spacechildo]
    #23689763 - 09/29/16 08:52 AM (7 years, 3 months ago)

Ya its from a tiger drop and like space said if you cut them into smaller pieces its faster maybe not even have to shake if its fast myc and even dispersed


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Re: Spawn clean vs dirty identification pictures [Re: Boogieman47]
    #23689950 - 09/29/16 10:08 AM (7 years, 3 months ago)

Great info here guys thank you


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InvisibleJacobStorm
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Re: Spawn clean vs dirty identification pictures [Re: PortabellaFella 1]
    #23690049 - 09/29/16 10:33 AM (7 years, 3 months ago)

Thats fucking awesome. So is a fully colonized agar plate resistant to contam and bactiriea? Like a fully colonized BRF cake?


--------------------
Rogger Rabbits Mushroom growing videos

Ethnobotanical garden forum

Inocuole tea TEK

azur said: If you like 6th grade results, then 6th grade projects are great.


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InvisiblebodhisattaMDiscordReddit
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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
    #23690053 - 09/29/16 10:35 AM (7 years, 3 months ago)

No, agar is used as an inoculant so it has to stay clean and sterile.
Cakes are resistant after birthing yes but you don't use them to inoculate something.


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Offlinespacechildo
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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
    #23690057 - 09/29/16 10:35 AM (7 years, 3 months ago)

Quote:

JacobStorm said:
Thats fucking awesome. So is a fully colonized agar plate resistant to contam and bactiriea? Like a fully colonized BRF cake?




eh.. you dont want mold spores on your colonized agar plates, the grains you put said wedge on wont like the contams.

cutting your agar dish is a contam vector, dirty scalpel, poor technique etc, tigerdropping eliminates that vector.


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InvisibleJacobStorm
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Re: Spawn clean vs dirty identification pictures [Re: bodhisatta]
    #23690109 - 09/29/16 10:50 AM (7 years, 3 months ago)

Quote:

spacechildo said:
Quote:

JacobStorm said:
Thats fucking awesome. So is a fully colonized agar plate resistant to contam and bactiriea? Like a fully colonized BRF cake?




eh.. you dont want mold spores on your colonized agar plates, the grains you put said wedge on wont like the contams.

cutting your agar dish is a contam vector, dirty scalpel, poor technique etc, tigerdropping eliminates that vector.




Right so its not tigerdropping unless we chop it up. This is great!
Quote:

bodhisatta said:
No, agar is used as an inoculant so it has to stay clean and sterile.
Cakes are resistant after birthing yes but you don't use them to inoculate something.




Yeah I get that it makes sense.


--------------------
Rogger Rabbits Mushroom growing videos

Ethnobotanical garden forum

Inocuole tea TEK

azur said: If you like 6th grade results, then 6th grade projects are great.


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InvisibleBoogieman47
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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
    #23690116 - 09/29/16 10:53 AM (7 years, 3 months ago)

No tiger drop is tge whole piece dropped in tic tac tiger drop is cutting it


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Re: Spawn clean vs dirty identification pictures [Re: JacobStorm]
    #23690150 - 09/29/16 11:03 AM (7 years, 3 months ago)

:whathesaid:

cutting the agar plate is "normal".
not cutting it is called tiger drop here, after elasticaltiger.


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Re: Spawn clean vs dirty identification pictures [Re: spacechildo]
    #23690177 - 09/29/16 11:11 AM (7 years, 3 months ago)

Has anyone heard from him ? Just wondering how hew doing?


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Re: Spawn clean vs dirty identification pictures [Re: Boogieman47]
    #23703980 - 10/03/16 03:00 PM (7 years, 3 months ago)

Fuck


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Re: Spawn clean vs dirty identification pictures [Re: blackdust]
    #23704084 - 10/03/16 03:39 PM (7 years, 3 months ago)

:rofl:


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Re: Spawn clean vs dirty identification pictures [Re: spacechildo]
    #23704281 - 10/03/16 04:45 PM (7 years, 3 months ago)

Amazing idea bod.  Great post.  Cant wait for it to be finished.  Its actually sad we dont have something like this already after all these years.


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Re: Spawn clean vs dirty identification pictures [Re: RyeJar]
    #23704348 - 10/03/16 05:03 PM (7 years, 3 months ago)

Bods been picking up your slack 'round here RyeJar... if that's even your real name


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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Re: Spawn clean vs dirty identification pictures [Re: ComebackKid]
    #23704387 - 10/03/16 05:18 PM (7 years, 3 months ago)

There is a lot of slack on my part.  I only remember to come on this site and help out about two weeks a year.  I forgot me ol' password for my last account and that email is long gone.


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Re: Spawn clean vs dirty identification pictures [Re: bodhisatta]
    #23931491 - 12/16/16 03:53 AM (7 years, 1 month ago)

Update for Wheat Grain:

Wheat

Bacterial:





Right after shaking:



After 48 hours:



Healthy:



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Offlinewtfcrazymofo
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Re: Spawn clean vs dirty identification pictures [Re: enlightenment]
    #23931508 - 12/16/16 04:15 AM (7 years, 1 month ago)

PE6 on producers pride oats Hopefully clean


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineDactylium
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Re: Spawn clean vs dirty identification pictures [Re: wtfcrazymofo]
    #23931510 - 12/16/16 04:20 AM (7 years, 1 month ago)

>producer's pride
I forgot about Inocuole's warning against this brand and ended up with a bag full of weevils.


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Re: Spawn clean vs dirty identification pictures [Re: Dactylium]
    #23931715 - 12/16/16 08:33 AM (7 years, 1 month ago)

Those are just extra protein


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