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OfflineSmoothcat
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Re: I need help with a contamination problem. [Re: tombosley8]
    #23660666 - 09/20/16 04:18 AM (7 years, 4 months ago)

:whathesaid:

Bruising.


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OfflineKenetic
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Re: I need help with a contamination problem. [Re: Smoothcat]
    #23660691 - 09/20/16 04:45 AM (7 years, 4 months ago)

Do you use sterile water and sterile syringe to make the LI?

Top left corner of first pic looks a little off to me.  Kinda grey.

I'd agree that the blue on the stumps is bruising.


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InvisibleMad Season
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Re: I need help with a contamination problem. [Re: Kenetic]
    #23660882 - 09/20/16 07:29 AM (7 years, 4 months ago)

Top left corner is fine. Just some spores fell there. First pic right in the middle of the pic tho.. those white bubbly things.. that's going to turn green very soon, in fact it looks like there's already some trich sporulating just to the left of it. Definitely take some close-ups of this area OP.


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InvisibleBoogieman47
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Re: I need help with a contamination problem. [Re: Mad Season]
    #23661056 - 09/20/16 09:18 AM (7 years, 4 months ago)

The stumps are left over stems when you cut them


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InvisibleMad Season
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Re: I need help with a contamination problem. [Re: Boogieman47]
    #23661077 - 09/20/16 09:28 AM (7 years, 4 months ago)

:whathesaid:

but dude please take a close-up of these..



red is IMO unsporulated trich, green circle is already sporulating trich, IMO. Need closeups ASAP PLEASE.


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InvisibleBoogieman47
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Re: I need help with a contamination problem. [Re: Mad Season]
    #23661232 - 09/20/16 10:21 AM (7 years, 4 months ago)

Ya those look like they could be trich but i have a spot like that its been there over two weeks i think mine is just where myc grew after a heavy misting idk?? Good eye mad


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Offlinetedoro
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Re: I need help with a contamination problem. [Re: Boogieman47]
    #23661303 - 09/20/16 10:50 AM (7 years, 4 months ago)

OK,

There is other mold. Both photos show the beginning of a greyish contam.

But the stumps are rotting/becoming slimey.

This is the first I've heard of this... bruising. I use sharp scissors snips. And when I dunk, I flood the sub with water and tip it up vertical with a gloved hand to drain it out for a minute.

How can I improve?


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Offlinetedoro
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Re: I need help with a contamination problem. [Re: tedoro]
    #23661320 - 09/20/16 11:00 AM (7 years, 4 months ago)





close up from today


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Offlinetedoro
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Re: I need help with a contamination problem. [Re: tedoro]
    #23661329 - 09/20/16 11:02 AM (7 years, 4 months ago)

Oh, I get it, You are just saying that the stumps turn blue upon cutting. Yes I know that. No harm there, its the slime that ain't right. They aren't turning white again like they have for me in the past.


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Offlinetombosley8
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Re: I need help with a contamination problem. [Re: tedoro]
    #23661330 - 09/20/16 11:03 AM (7 years, 4 months ago)

yes that is trich toss it, sorry


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Offlinetombosley8
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Re: I need help with a contamination problem. [Re: tombosley8]
    #23661343 - 09/20/16 11:08 AM (7 years, 4 months ago)

as said before, this is most likely because of dirty spawn which either had trich in the first place or bacteria which weakened the sub and let the mold set in.

Yeah the slimyness sounds like bacteria...

How long are you dunking for?

maybe cut back a little but that won't solve the problem. You need to focus on find the contam vectors and eliminating them.


Are you sure your agar cultures are clean?(if you are using agar if not start now)


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Offlinetedoro
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Re: I need help with a contamination problem. [Re: tombosley8]
    #23661378 - 09/20/16 11:15 AM (7 years, 4 months ago)

I'm using agar.

I know its toast, thats why I'm here trying to fix it.

So I don't need to change how I'm harvesting, correct?

But I will attempt to get the spawn healthier.

I thought I had it figured out when I learned I wasn't PC'ing my rye long enough. The Big bags need a long ol' time. But that didn't fix it.

Now I'll try removing the blender made LI, make it the old school way in the dish.

I'll post my expanding growth in the bags, to see if any of you can spot ill health.

Many many thanks for your brainstorming, I really appreciate it.


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InvisibleMad Season
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Re: I need help with a contamination problem. [Re: tombosley8]
    #23661389 - 09/20/16 11:18 AM (7 years, 4 months ago)

:whathesaid: damn not sure if it's a good thing I can spot unsporulated trich from so far away :facepalm:

Yes up the PC times, ALWAYS have uninoculated control jars to test the lids/filters, and always have clean inoculant. If you do these things and ensure it's fine, you won't have this problem.

Stumps going bacterial and shit is also from bad spawn. They're just as much a part of the mycelial network as anything else. Clean spawn is the key!


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contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (09/20/16 11:20 AM)


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Offlinetedoro
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Re: I need help with a contamination problem. [Re: tedoro]
    #23661396 - 09/20/16 11:21 AM (7 years, 4 months ago)

And yes, my blender procedure was somebodies tek on this forum.. PC'd water in a jar with lid/blade contraption as the lid. Self-healing port. Now I think I'm messing that up somehow.


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Offlinetedoro
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Re: I need help with a contamination problem. [Re: tedoro]
    #23661404 - 09/20/16 11:23 AM (7 years, 4 months ago)

Uninnoculated controls. Now thats some thinking right there.


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Offlinetombosley8
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Re: I need help with a contamination problem. [Re: tedoro]
    #23661407 - 09/20/16 11:24 AM (7 years, 4 months ago)

fuck self healing ports!

crack the lid for the win!!!


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OfflineOregonic
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Re: I need help with a contamination problem. [Re: tedoro]
    #23661413 - 09/20/16 11:26 AM (7 years, 4 months ago)

Yeah OP if the "stump-rotting and then mold" is what you're depicting in those photos, it just looks like regular ol' bruising to me.


Also, are those exposed grains visible in the first pic?


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InvisibleBoogieman47
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Re: I need help with a contamination problem. [Re: Oregonic]
    #23661428 - 09/20/16 11:32 AM (7 years, 4 months ago)

Trust me mad ive had my fair share of trich haha i agree use poly and open the lid but i havent heard self healing ports are bad unless you use rtv ... i take the ring off and wipe the jar beforw opening the main lid it helps alot


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Offlinetedoro
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Re: I need help with a contamination problem. [Re: Boogieman47]
    #23661436 - 09/20/16 11:35 AM (7 years, 4 months ago)

It does seem like I sometimes see exposed grains if there is a little bit of tearing from a harvest. Whats that about?


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InvisibleBoogieman47
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Re: I need help with a contamination problem. [Re: tedoro]
    #23661451 - 09/20/16 11:39 AM (7 years, 4 months ago)

They dry out faster and can be contam vector but being your second flush i wouldnt worry you already have trich haha take that tub outside or isolate it in another room finish those pins then out to the heap it goes


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