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Offlinetedoro
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I need help with a contamination problem.
    #23659755 - 09/19/16 06:59 PM (7 years, 4 months ago)

Franks Monos with Magnetic swappable filters | Petri dishes with numerous transfers | Pre-sealed Myco Bags nocced with blender made LI with syringe | coco/verm substrate with improved bucket tek | Golden teacher | rye berries | misting/fanning irregularly

Hi,

I've been doing monotubs for years, and shotgun jars before that. Still a novice though, I know my place.

So I asking for some help.

I am getting contams after my first harvest, most of the time. I'm only getting 3 ounces dry from a tub with over 3 quarts of rye grains. My stumps are often rotting. Then mold. (see attached photos (hours after a dunk)

My ideas for the possible cause:

1) Someone smart in the forum recently spoke of the possibility that bacteria is fighting mycelia during the grain consolidation, making it weak, but not visibly so - causing it to be prone to disease.

2) My bucket tek (temps measured and timed, then cooled quickly).... is inadequate. Need to pasteurize my sub better.

3) My myco bags suck.

4) Something about the environment I'm providing is less than desirable (sub moisture, FAE, misting/fanning, spawn/sub mixing cleanliness)

Hit me with your gut instincts. I want to fix this. Thanks in advance.





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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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OfflineBobabouy
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Re: I need help with a contamination problem. [Re: tedoro]
    #23659762 - 09/19/16 07:05 PM (7 years, 4 months ago)

Your spawn is the culprit.


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Invisibleamidogen
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Re: I need help with a contamination problem. [Re: tedoro]
    #23659765 - 09/19/16 07:05 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 10:17 AM)


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Offlinetedoro
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Re: I need help with a contamination problem. [Re: amidogen]
    #23659787 - 09/19/16 07:15 PM (7 years, 4 months ago)

My spawn prep:

Agar dishes, numerous transfers... clean looking to me.

Blender made LI

3.5 quarts of rye in a bag, presealed. four bags in a pressure cooker, careful wooden spacers to circulate steam. 3.5 hours.

Alcohol spot on bag, tape that, more alcohol, flame the needle, inject 15 mm of LI. quickly tape hole. shake.

It takes over the bag, but not real fast. I have been seeing growth starting in 6 days or so. Perhaps 2.5 weeks to be complete.

I recently learned that my practice of cooling my agar petri dished before making the LI weakens the mycelia, even if I let it recover at room temp for a day. I'm changing that now.

I am very fond of pre-sealing the bag, because all I have is a crummy Still Air Box, and love seeing the bag all sealed when it comes out of the pressure cooker.


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--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
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Re: I need help with a contamination problem. [Re: amidogen]
    #23659802 - 09/19/16 07:19 PM (7 years, 4 months ago)

crummy SAB?  a shit one is awesome. dont hate

id just transfer wedges of agar. LI sounds cool but its another step that
can introduce contams..

also if it goes sour after 1 flush your spawn is fucked.


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Offlinetedoro
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Re: I need help with a contamination problem. [Re: mushboy]
    #23659815 - 09/19/16 07:23 PM (7 years, 4 months ago)

I have a still air box

OK. I'd like to see if I can fix my current procedure before I change things up too much.

Is LI injected into a myco bag a really bad idea?


--------------------
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Extras: Filter Print Post Top
Offlinetedoro
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Re: I need help with a contamination problem. [Re: tedoro]
    #23659820 - 09/19/16 07:26 PM (7 years, 4 months ago)

I get creeped out bringing an unsealed myco bag out of a PC, opening it in a loving beautuful SAB, then dragging it over to a sealer. Doesn't sound so cool in my not so clean apartment.


--------------------
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Extras: Filter Print Post Top
Offlinetedoro
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Re: I need help with a contamination problem. [Re: tedoro]
    #23659827 - 09/19/16 07:28 PM (7 years, 4 months ago)

I've also wondered about the heartiness of the filter patch to long PC times.


--------------------
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Extras: Filter Print Post Top
OfflineBobabouy
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Re: I need help with a contamination problem. [Re: tedoro]
    #23659834 - 09/19/16 07:31 PM (7 years, 4 months ago)

I would guess that if your plates really are clean, the problem is somewhere in the process of making the LI.


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Offlinetedoro
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Re: I need help with a contamination problem. [Re: Bobabouy]
    #23659838 - 09/19/16 07:32 PM (7 years, 4 months ago)

That helps me out a lot. I'll focus on that.


--------------------
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Offlinetedoro
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Re: I need help with a contamination problem. [Re: tedoro]
    #23659841 - 09/19/16 07:34 PM (7 years, 4 months ago)

Maybe I'll try a squirt and scrape in the petri dish method.... it has worked well in the past.


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
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Re: I need help with a contamination problem. [Re: tedoro]
    #23659844 - 09/19/16 07:34 PM (7 years, 4 months ago)

Quote:

tedoro said:
I get creeped out bringing an unsealed myco bag out of a PC, opening it in a loving beautuful SAB, then dragging it over to a sealer. Doesn't sound so cool in my not so clean apartment.




use a clothes pin?


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Offlinetedoro
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Re: I need help with a contamination problem. [Re: tedoro]
    #23659845 - 09/19/16 07:34 PM (7 years, 4 months ago)

I have a little rust in the tin of my blender contraption thingy.


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Offlinetedoro
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Re: I need help with a contamination problem. [Re: tedoro]
    #23659849 - 09/19/16 07:36 PM (7 years, 4 months ago)

Another thing about that open bag thing in the PC.... doesn't the PC, when cooling, suck a bunch of room air into the chamber?


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Extras: Filter Print Post Top
OfflineBobabouy
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Re: I need help with a contamination problem. [Re: tedoro]
    #23659854 - 09/19/16 07:37 PM (7 years, 4 months ago)

You can also give GLC a shot. I don't do bags but I know plenty of people PC unsealed bags.


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Offlinethemadextractor
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Re: I need help with a contamination problem. [Re: Bobabouy]
    #23659875 - 09/19/16 07:44 PM (7 years, 4 months ago)

I'd guess either the bags or the LI. I would try a simpler method for the LI. I fucked an entire round of jars up tying the blender method just due to poor technique. I now do blender less LI. Works better with less vectors. Just do in and shake the agar with water. I'd avoid GLC as it is contam prone.


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OfflineBobabouy
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Re: I need help with a contamination problem. [Re: themadextractor]
    #23659899 - 09/19/16 07:55 PM (7 years, 4 months ago)

If I were to do bags, I would grain to grain them.


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InvisibleBoogieman47
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Re: I need help with a contamination problem. [Re: Bobabouy]
    #23659957 - 09/19/16 08:19 PM (7 years, 4 months ago)

All those look like to me is bruised stumps?


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Offlinetedoro
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Re: I need help with a contamination problem. [Re: Boogieman47]
    #23660559 - 09/20/16 02:31 AM (7 years, 4 months ago)

bruised stumps? what do you mean? I cut them with clean sharp snips...


--------------------
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


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Offlinetombosley8
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Re: I need help with a contamination problem. [Re: Boogieman47]
    #23660610 - 09/20/16 03:24 AM (7 years, 4 months ago)

mushroom tissue will bruise from even the slightest of pressure so your cuts are what is causing the blue, NOT MOLD.

Unless there is more that your not showing...

Mold that I have seen on stumps(actually never seen a blue mold in mush cult) is always green and doesn't show that fast.

It looks pretty healthy maybe slightly bacterial but just keep an eye on it and you should be good,
Quote:

noob47 said:
All those look like to me is bruised stumps?




I totally agree.


OP, keep us updated with pics in the next couple days so we can be sure...

That's when I would usually see mold if my spawn were crap.


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