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OfflineAtuan
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Registered: 07/23/14
Posts: 13
Last seen: 7 years, 4 months
First try Agar
    #23654746 - 09/18/16 03:08 AM (7 years, 4 months ago)



These don't look at all like I expected, is this contamination?
Also, the method I used to reduce condensation didn't do much :/


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InvisibleSuperFly
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Registered: 05/19/13
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Re: First try Agar [Re: Atuan]
    #23654753 - 09/18/16 03:14 AM (7 years, 4 months ago)

That's bacteria


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OfflineAtuan
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Re: First try Agar [Re: SuperFly] * 1
    #23654768 - 09/18/16 03:35 AM (7 years, 4 months ago)

Are the white blotches in the two plates mycelium? Why don't I see any myc? :/

Bacteria beat it up?


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Offlinemrmazdarx9
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Re: First try Agar [Re: Atuan]
    #23654773 - 09/18/16 03:39 AM (7 years, 4 months ago)

I can't see much myc there but it could be the light photo quality etc. Did you use ms syringe I'm guessing so due to the runs. Better pics would make it easier to make out. It does look bacterial in those pics


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Offlinewtfcrazymofo
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Re: First try Agar [Re: Atuan]
    #23654814 - 09/18/16 05:02 AM (7 years, 4 months ago)

how old? how many drips?


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If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineAtuan
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Re: First try Agar [Re: wtfcrazymofo]
    #23654822 - 09/18/16 05:09 AM (7 years, 4 months ago)

About 3 days now. Only a drop or two in each one. Already scrapped them. Bought lots of ingredients expecting this. I wonder if its my inoculation or making the syringe from a jar of spore water.


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Offlinemrmazdarx9
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Re: First try Agar [Re: Atuan]
    #23654838 - 09/18/16 05:26 AM (7 years, 4 months ago)

3 days yeah that's not good will take much longer than that. Did you make the syringe? It looks like where the water ran is bacterial. Did you use sterilised water?


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OfflineAtuan
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Re: First try Agar [Re: mrmazdarx9]
    #23654870 - 09/18/16 06:16 AM (7 years, 4 months ago)

I should just look for an apprenticeship


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Invisibleblindingleaf
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Re: First try Agar [Re: Atuan]
    #23654877 - 09/18/16 06:30 AM (7 years, 4 months ago)

these boards are almost like an apprenticeship of sorts….u get live feedback in real time at least.

you barely have condensation on there,so don't worry about that.

ur syringe has bacteria, like they mentioned above.  your plates don't look like they are wrapped with anything, so thats not good either.

i can't tell what the white is, but if it is cubes, u need to get it out of there and onto another plate ASAP.


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InvisibleMycolorado
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Re: First try Agar [Re: Atuan]
    #23654989 - 09/18/16 08:32 AM (7 years, 4 months ago)

Hey Atuan, you should figure out if it's your plates or your syringe that are contaminated(or your technique).  Do you have any plates that are not inoculated and are they contaminated?  If so, you know your plates are bad.  How do you make your agar and how do you pour the plates?  Also, if you're making the syringes yourself, why not just use a loop to transfer some spores from your print to the agar?


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Offlinemrmazdarx9
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Re: First try Agar [Re: blindingleaf]
    #23655010 - 09/18/16 08:47 AM (7 years, 4 months ago)

Quote:

blindingleaf said:
but if it is cubes, u need to get it out of there and onto another plate ASAP.



Whoops just reminded me I've got APE and PEU to transfer today forgot all about them


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OfflineMushierage
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Re: First try Agar [Re: mrmazdarx9]
    #23655169 - 09/18/16 10:00 AM (7 years, 4 months ago)

Instead of dropping spore solution on the plate, drop it on a sterilized swab.  Then swab a z in the middle of your plate. 

You will have much better results this way. 

Or just use a spore print to swab it instead of spore solution.


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineGreg
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Re: First try Agar [Re: mrmazdarx9]
    #23655193 - 09/18/16 10:09 AM (7 years, 4 months ago)

Quote:

mrmazdarx9 said:
Quote:

blindingleaf said:
but if it is cubes, u need to get it out of there and onto another plate ASAP.



Whoops just reminded me I've got APE and PEU to transfer today forgot all about them




I just transferred some APE!
My PEU refuses to germinate though :frown:


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Offlinemrmazdarx9
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Re: First try Agar [Re: Greg]
    #23655205 - 09/18/16 10:17 AM (7 years, 4 months ago)

Quote:

Greg said:
Quote:

mrmazdarx9 said:
Quote:

blindingleaf said:
but if it is cubes, u need to get it out of there and onto another plate ASAP.



Whoops just reminded me I've got APE and PEU to transfer today forgot all about them




I just transferred some APE!
My PEU refuses to germinate though :frown:



Yeah PEU takes ages ive just done my first transfer on it and second transfer on APE both were started at the same time. A little over two weeks before I saw any action at all on the PEU by that I mean a tiny spec appeared after two weeks, I was ready to bin the plate.


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