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Offlinemellyjane
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In Vitro Plastic Tek Question
    #23653382 - 09/17/16 03:13 PM (7 years, 4 months ago)

Tried Violets Plastic In vitro telk. I thought it was fully colonized and so cracked lid with indirect light for over a week. No pins that I can tell.



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Offlinemellyjane
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Re: In Vitro Plastic Tek Question [Re: mellyjane]
    #23653396 - 09/17/16 03:17 PM (7 years, 4 months ago)

Any and all advice appreciated. Does this look normal?


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Offlinefunton
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Re: In Vitro Plastic Tek Question [Re: mellyjane]
    #23653710 - 09/17/16 05:16 PM (7 years, 4 months ago)

What is in there?  It looks healthy....


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Offlinefunton
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Re: In Vitro Plastic Tek Question [Re: funton]
    #23653746 - 09/17/16 05:26 PM (7 years, 4 months ago)

My last invitro jar



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OfflineKenetic
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Re: In Vitro Plastic Tek Question [Re: funton]
    #23653805 - 09/17/16 05:47 PM (7 years, 4 months ago)

ouch


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OfflineKenetic
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Re: In Vitro Plastic Tek Question [Re: mellyjane]
    #23653817 - 09/17/16 05:51 PM (7 years, 4 months ago)

Quote:

mellyjane said:
and so cracked lid with indirect light for over a week.





What does this mean?

I would suggest putting the cups in a monotub an removing the lids altogether.  You have a lot of moisture that could evaporate that way and trigger some pins.


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Todo Cambia
   

               
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Offlinemellyjane
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Re: In Vitro Plastic Tek Question [Re: Kenetic]
    #23653841 - 09/17/16 06:03 PM (7 years, 4 months ago)

What's in there is a Texas strain

The tek I followed suggests cracking the lid to allow for FAE and maintain humidity. So I did that and set in place to get some indirect sunshine


I like your idea.


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Offlinemellyjane
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Re: In Vitro Plastic Tek Question [Re: mellyjane]
    #23653851 - 09/17/16 06:09 PM (7 years, 4 months ago)



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OfflineMushierage
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Re: In Vitro Plastic Tek Question [Re: mellyjane] * 1
    #23653859 - 09/17/16 06:14 PM (7 years, 4 months ago)

The pods tek does call for cracking the lid.  However I believe some better teks have been developed since then.  I would just follow the tek, and use your mono for something more efficient.  Like a bulk substrate. 

Try searching for the Big Gulp Tek.  I hear it works very well.


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineKenetic
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Re: In Vitro Plastic Tek Question [Re: mellyjane]
    #23653876 - 09/17/16 06:21 PM (7 years, 4 months ago)

I've seen that tek before.  This is what I'm talking about:

In a sgfc

That pic is off one of pasty's posts and shows the cups in a sgfc.  I've also seen the same thing in a monotub with good results but I can't find a pic right now.

I'm not suggesting going away from her tek but I think the cups would do better in a large container with some fae.


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineMushierage
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Re: In Vitro Plastic Tek Question [Re: Kenetic]
    #23653888 - 09/17/16 06:25 PM (7 years, 4 months ago)

I also agree however, the tek would do probably do better in an SGFC than anything.  The jar itself will provide a microclimate, so humidity shouldn't be a problem.  FAE is probably the single biggest issue with that tek. 

But unless you have ten of these things, I believe it to be a little counter productive to use an SGFC at all. 

:shrug:


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineKenetic
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Re: In Vitro Plastic Tek Question [Re: Mushierage]
    #23653923 - 09/17/16 06:41 PM (7 years, 4 months ago)

so how many cups do you have?


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Offlinefunton
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Re: In Vitro Plastic Tek Question [Re: Kenetic]
    #23655548 - 09/18/16 12:31 PM (7 years, 4 months ago)

Just have patience, it'll pin soon. I've grown many times invitro for fun.... that pic I posted was after last tubs I did I just knocked all the left over WBS in all the jars into that one and dumped in a little coir and put the lid back on.  Same idea as that tech, it should pin very soon.  I just take the lid off about once a day and fan some fresh air in, if the lids don't have any form of air exchange then yeah I'd leave it cracked a little. 

The picture I posted looked bad because I never intended to pick them, I like watching them complete their life cycle.... those looked great several days ago lol.


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InvisiblemushboyMDiscord
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Re: In Vitro Plastic Tek Question [Re: funton]
    #23655609 - 09/18/16 12:47 PM (7 years, 4 months ago)

fanning?

fresh air exchange is not fanning nor does one replace the other.


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Offlinefunton
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Re: In Vitro Plastic Tek Question [Re: mushboy]
    #23655624 - 09/18/16 12:53 PM (7 years, 4 months ago)

Dude, we are just talking about jars..... They are gonna grow with virtually no air even.  If we were talking about monotubs, etc... I'd be more inclined to discuss it with you.  But I've grown tons of cubes invitro and accidentally achieved good results here and there considering I just use the "waste" that didn't go into tubs.  So that being said, I can't scientifically say that fanning the jars helped.... but I can say it didn't hurt and since most of the jars I fruit in only uses the GE filter for air exchange, I like to fan them some.

But I also will say I don't really care about yield that much as I typically just let them spore and die away like in that pic I posted...so I never really care if conditions are "ideal"


Edited by funton (09/18/16 12:55 PM)


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OfflineMushierage
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Re: In Vitro Plastic Tek Question [Re: funton]
    #23655701 - 09/18/16 01:23 PM (7 years, 4 months ago)

Well, I don't know about you, but I don't grow any mushrooms just to let them die off. 

Seems like a senseless waste to me.  Even if it is just a couple mushies.


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineAK1000
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Re: In Vitro Plastic Tek Question [Re: mellyjane]
    #23655705 - 09/18/16 01:24 PM (7 years, 4 months ago)

Quote:

mellyjane said:
Tried Violets Plastic In vitro telk. I thought it was fully colonized and so cracked lid with indirect light for over a week. No pins that I can tell.





Same here! Looks like my jar, which the lid has been loosened for 16 days thus far and no pins... yet. But I'm doing PE strain and I hear it takes forever to pin.



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OfflineMushierage
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Re: In Vitro Plastic Tek Question [Re: AK1000]
    #23655712 - 09/18/16 01:28 PM (7 years, 4 months ago)

PE huh?

You're gonna wanna give that thing a load of FAE or it's gonna blob to hell and back.  Probably will anyway.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Offlinefunton
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Re: In Vitro Plastic Tek Question [Re: AK1000]
    #23655732 - 09/18/16 01:33 PM (7 years, 4 months ago)

Quote:

Well, I don't know about you, but I don't grow any mushrooms just to let them die off.

Seems like a senseless waste to me.  Even if it is just a couple mushies.





Well you kind a do know about me letting mushrooms die off because I just told you I do.  I enjoy growing them for hobby, I also like growing weird plants and stuff sometimes, and they eventually die. Nothing is senseless if I gained joy in seeing them from start to finish.  And even if it is a couple mushies.... LOL you'd probably freak if you knew how many I let die, because I love growing them but have nothing to do with them once they are grown. I want to get into edibles so that I can atleast give those away to people but I like the simplicity of cubes.  ¯\_(ツ)_/¯


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OfflineMushierage
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Re: In Vitro Plastic Tek Question [Re: funton]
    #23655789 - 09/18/16 01:52 PM (7 years, 4 months ago)

Right.  Because that's a great way to use an illegal felony drug after spending time and effort to subvert laws that threaten your freedom. 

Whatever floats your boat guy.  Have fun with that.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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