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Offlinedimtis
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Registered: 05/07/16
Posts: 11
Last seen: 6 years, 1 month
BRF Cake not colonizing
    #23652146 - 09/17/16 04:28 AM (7 years, 4 months ago)

Hello all,
Up to now I've got my cakes colonized and fruited about five times. First, I colonized PDA agar from spore print, then I put colonized agar slices on cakes to start cake colonization. I use laminar hood and autoclave, and no other contamination observed within the cakes. Few days after agar slice inoculation, there's a strange smell (like wine)and colonization doesn't occur anymore. I suspect from oxygen depletion causing this smell and growth suppression. My jars are deep, although I fill 1/3 of the jars with 2:1:1 brf cake ratio (as I always do), could this be an oxygen issue?


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Offlinetump
ban the undead
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Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
Re: BRF Cake not colonizing [Re: dimtis]
    #23652162 - 09/17/16 04:43 AM (7 years, 4 months ago)

Could be a yeast or bacteria problem. But i have to know because my brain hurts thinking about this otherwise. You have a flowhood. You have a autoclave. Why aren't you making grain spawn.


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OfflineMushierage
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Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
Re: BRF Cake not colonizing [Re: tump]
    #23652731 - 09/17/16 10:42 AM (7 years, 4 months ago)

I agree with the grain spawn comment.  Using agar with PF cakes seems a little counter productive to me, that's why the tek involves shooting spores directly into the substrate. 

If you're going to use agar, move to grain and spawn to bulk.  You will have much better yields anyway. 

Just make sure to transfer clean sectors of myc to other plates.  And the once you get a clean one, tiger drop 2.0 that sucker in and get yourself a clean grain master.  Then just g2g to expand.  :shrug:


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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