|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Smoothcat
Renegade-master



Registered: 10/07/15
Posts: 1,254
Last seen: 5 months, 28 days
|
First time millet
#23650514 - 09/16/16 01:24 PM (7 years, 4 months ago) |
|
|
Ok here's a question, looking at fooman's tek on wbs it requires a quick slack in boiled water and then pc'd for 45mins.
My question is this, is 45 mins long enough?
I thought that you needed at least 90mins with grain but I can't find anyone stating otherwise with this tek.
Reason being I'm trying 3 jars with this tek and 3 jars with another and I just want to do one pc run at 90mins
Any help welcome thanks all
-------------------- Back once again with the ill behaviour Links I like
|
tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 19 hours, 55 minutes
|
Re: First time millet [Re: Smoothcat]
#23650528 - 09/16/16 01:28 PM (7 years, 4 months ago) |
|
|
i do 90 i do 120
|
Smoothcat
Renegade-master



Registered: 10/07/15
Posts: 1,254
Last seen: 5 months, 28 days
|
|
Yeah I thought 45 was too short,
Well they in the pc now they gonna get 100 Hahahaha!!!!
-------------------- Back once again with the ill behaviour Links I like
|
Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
|
|
do 120 for best results, at least 90 but not 45 with grains
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
Re: First time millet [Re: Tira]
#23650911 - 09/16/16 04:26 PM (7 years, 4 months ago) |
|
|
90min+ from the time it first hits 15psi.
|
george castanza
Lord Of The Idiots!


Registered: 10/21/02
Posts: 8,723
|
Re: First time millet [Re: Smoothcat]
#23650924 - 09/16/16 04:31 PM (7 years, 4 months ago) |
|
|
If you got the grain warm and wet for an extended period, say an hour or longer you have actually in all likelihood launched the endospores in your grain making them susceptible to death via sterilization. 45 minutes @15lbs for millet is acceptable if the grain was boiled before processing.
I like to soak millet in hot water overnight because I don't like fucking with boiling hot grain, but either way it works. I usually go a little longer than 45 min with soaked millet, not because it's really necessary but more because I'll spend a penny on prevention when I know it's a good buy over the price on a cure.
-------------------- KRAMER CAKES


|
Smoothcat
Renegade-master



Registered: 10/07/15
Posts: 1,254
Last seen: 5 months, 28 days
|
|
Well it's just done 100 at slightly above 15psi but not quite half way to 16psi
Three jars were soaked as per fooman's tek in boiling water for 20mins
The other three were with the ratio method with rinsed millet and water in the jar.
Three different lid designs for each method.
I'll be opening the pc up later. Hopefully the millet won't be over cooked. Anyone ever done that??
-------------------- Back once again with the ill behaviour Links I like
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: First time millet [Re: Smoothcat]
#23650949 - 09/16/16 04:45 PM (7 years, 4 months ago) |
|
|
One thing about millet is that the tiny grain size means the heat transfers quicker. But in my mind that's no reason to hold back, we want as many logs of overkill sterilization as the media can handle.
|
Smoothcat
Renegade-master



Registered: 10/07/15
Posts: 1,254
Last seen: 5 months, 28 days
|
|
My last four jars went bad green. They were bird seed mainly consisting of millet. I did them with the ratio tek.
The grains weren't washed or anything just loaded into jars then pc, 300 millet with 175 water and 1/4 tsp gypsum.
Pc'd for 90-100 mins, the grains didn't look over cooked but then I wasn't really sure of how that would look.
Dropped agar wedges into the jars and left for 10 days. All jars got green.
I want to find out why hence running this test.
My theory is that the lid design was whack. But it could have been the grain prep method or my sterile tech during innoc. 
I'm using new jars with three different lid designs using the two different grain preps. Hopefully this will help me hone a more reliable method. Plus I just got a shit load of millet.
-------------------- Back once again with the ill behaviour Links I like
|
george castanza
Lord Of The Idiots!


Registered: 10/21/02
Posts: 8,723
|
Re: First time millet [Re: Smoothcat]
#23651393 - 09/16/16 07:50 PM (7 years, 4 months ago) |
|
|
Green is most likely contamination in your inoculant, although unsterilized millet will most certainly grow some green, it will also grow a wide variation of contaminates. All of the jars being contaminated in the same manner with the same type of contamination points to the spawn more than the process. If it was the jars themselves that were not sterilized or your sterile technique was off, then there would have been at least some mycelium in at least one of the jars competing with all the other stuff.
-------------------- KRAMER CAKES


|
Smoothcat
Renegade-master



Registered: 10/07/15
Posts: 1,254
Last seen: 5 months, 28 days
|
|
Quote:
If it was the jars themselves that were not sterilized or your sterile technique was off, then there would have been at least some mycelium in at least one of the jars competing with all the other stuff
There was good strong mycelium growth in those jars, big chunky myc probably about 40% but then there were big patches of evil green too should have got pics but I just dumped em outside so who knows...
Here's the current jars tho out the pc, two different grain preps, three lids.


The ones with the small piece of micro pore have 'ratio' written on them as they were done with the ratio tek, the others as per fooman's.
When the first came out the PC I thought they were definitely fucked as they all looked soaking. I read that can be common so I've shook them to disperse the moisture a few times since they have come out and they look a lot better now, but I'm not completely satisfied.
This is a close up of a ratio jar.

Seems like maybe the grains have been over cooked or over hydrated I'm not sure. A few burst grains as well. A little bit 'clumpy' but not as bad as when it first came out of the PC. The grains do not stick to the sides at all.
Here is a close up as per fooman's.

When they first came out I thought the were fucked because they seemed too wet and stuck to the sides of the jars like crazy. After a few shakes to disperse the moisture they don't stick much at all. These grains don't seem as hydrated as the other ones tho 
My plan is now to wait a day or two before inoculation and shake occasionally to disperse moisture. Not sure how these are gonna turn out...
Any thoughts?
-------------------- Back once again with the ill behaviour Links I like
|
george castanza
Lord Of The Idiots!


Registered: 10/21/02
Posts: 8,723
|
Re: First time millet [Re: Smoothcat]
#23653952 - 09/17/16 06:58 PM (7 years, 4 months ago) |
|
|
Well, I guess you need to review you're entire process. I certainly wouldn't wait, if anything would just trash them and start over.
I like mixing in some vermiculite with my millet. Makes it real easy to get the water content right. As far as your inoculation process, well that's kind of where you win or lose. You either do it right and get clean inoculation or fuck it up and wind up with contamination.
Keep at it.
-------------------- KRAMER CAKES


|
Smoothcat
Renegade-master



Registered: 10/07/15
Posts: 1,254
Last seen: 5 months, 28 days
|
|
So coming back to this thread.
I went with the advice and tossed 4 out of the 6 jars but out of curiosity I decided to nock up two of the jars with an agar wedge. And here they are now.

The one that appeared a bit dry has shown reasonable growth. Not the healthiest looking but no signs of contams as yet.

And the one that appeared too wet has shown strong healthy looking growth.

My first time with millet so still trying to hone my technique. Will probably shake these jars in the next day or so. My plan is to use them for G2G if/when they fully colonise.
Just thought I'd share.
Peace
-------------------- Back once again with the ill behaviour Links I like
|
|