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kaius711
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Registered: 09/15/16
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Last seen: 6 years, 1 month
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Assistance in using agar for pf tek
#23649576 - 09/16/16 05:19 AM (7 years, 6 months ago) |
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Greetings. I am preparing for my very first cultivation so i would ask you to bear with my ignorance. I have decided to use Pf Tek and a SGFC for my first cultivation however i was also advised to create an agar colony to use for the future. Having purchased a 10ml syringe i will use 8ml for my cakes and the last 2ml on agar. my question is how will i use this agar to create more cakes (cloning or multiply spores) as well as a request for general advice such as where to keep the agar. I appreciate your assistance.
Edited by kaius711 (09/16/16 05:21 AM)
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tump
ban the undead
Registered: 03/17/16
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Re: Assistance in using agar for pf tek [Re: kaius711]
#23649671 - 09/16/16 06:58 AM (7 years, 6 months ago) |
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Keep agar in still air box. Second make a lot of plates at same time. You start off with your geration plates. Make a few tranfers at first sign of grow. Keep tranfering until you get clean growth. Same with cloneing pins. When you have clean cultures you make li. Then inject li like you did spores. If you can master all that then you should shot for grain spawn.
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Leftfield420
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Re: Assistance in using agar for pf tek [Re: kaius711]
#23649877 - 09/16/16 08:38 AM (7 years, 6 months ago) |
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Use 1 or 2 drops of your syringe....2mls is waaaay too much....once your agar plate germinates..make a couple more transfers to new agar plates and use Munch's blenderless LI to inoculate jars.....that will go further and help with cutting down on contamination greatly... .you will need a SAB..
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kaius711
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Last seen: 6 years, 1 month
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Re: Assistance in using agar for pf tek [Re: Leftfield420]
#23649905 - 09/16/16 08:52 AM (7 years, 6 months ago) |
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I appreciate the advice however i have been told that as my cultivation level will be low (2-3 SGFC aiming for around 100 dried grams every 6 weeks) agar is not necessary. instead i have been advised to simply make spore prints and make new syringes. what are your opinions on this?
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Leftfield420
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Re: Assistance in using agar for pf tek [Re: kaius711]
#23649911 - 09/16/16 08:55 AM (7 years, 6 months ago) |
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Just making syringes will work....but more than likely will be dirty to some point and will cause contaminations eventually....maybe not every time but your success will be greater if you do decide to do agar work...
Edited by Leftfield420 (09/16/16 08:57 AM)
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amidogen
see you on the other side
Registered: 05/07/16
Posts: 1,782
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Re: Assistance in using agar for pf tek [Re: kaius711]
#23649915 - 09/16/16 08:59 AM (7 years, 6 months ago) |
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.
-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 10:10 AM)
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kaius711
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Re: Assistance in using agar for pf tek [Re: Leftfield420]
#23649918 - 09/16/16 08:59 AM (7 years, 6 months ago) |
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Even with proper sterilisation and caution? Which part is usually the most dangerous part for contamination when creating spore prints and transfering it into a syringe? I am assuming the spore print itself cannot be contaminated so when loading it up into the syringe?
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kaius711
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Re: Assistance in using agar for pf tek [Re: amidogen]
#23649924 - 09/16/16 09:03 AM (7 years, 6 months ago) |
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Acquiring agar itself might be a problem for me. i am unable to find it in the shops and online is not an option. i have heard of home made agar however it just sounds dodgy af.
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Leftfield420
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Re: Assistance in using agar for pf tek [Re: kaius711]
#23649925 - 09/16/16 09:03 AM (7 years, 6 months ago) |
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Your fruits are not grown in a sterile environment therefore your print is not 100% clean, when you suck spores up into a syringe you're loading everything that was stuck to the cap you printed
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amidogen
see you on the other side
Registered: 05/07/16
Posts: 1,782
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Re: Assistance in using agar for pf tek [Re: kaius711]
#23649926 - 09/16/16 09:03 AM (7 years, 6 months ago) |
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.
-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 10:10 AM)
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Leftfield420
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Re: Assistance in using agar for pf tek [Re: kaius711]
#23649928 - 09/16/16 09:04 AM (7 years, 6 months ago) |
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Asian food markets carry it....
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kaius711
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Re: Assistance in using agar for pf tek [Re: amidogen]
#23649929 - 09/16/16 09:06 AM (7 years, 6 months ago) |
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i understand that the gills might have been contaminated, but how are clean spore syringes created? Im starting off with a spore syringe so are you saying that all spore syringes are contaminated to a degree?
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kaius711
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Re: Assistance in using agar for pf tek [Re: Leftfield420]
#23649935 - 09/16/16 09:07 AM (7 years, 6 months ago) |
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really? that seems very unbelievable but i hope thats true. You wouldnt be talking about asian food markets in the nsw sydney region would you?
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amidogen
see you on the other side
Registered: 05/07/16
Posts: 1,782
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Re: Assistance in using agar for pf tek [Re: Leftfield420]
#23649941 - 09/16/16 09:09 AM (7 years, 6 months ago) |
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.
-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 10:10 AM)
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NumeroEno
I come from the land of lizards
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Posts: 9,652
Loc: Gamehendge
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Re: Assistance in using agar for pf tek [Re: kaius711]
#23649945 - 09/16/16 09:10 AM (7 years, 6 months ago) |
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Health food stores carry agar powder too. It's a vegan gelatin substitute and thickener.
-------------------- Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Leftfield420
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Re: Assistance in using agar for pf tek [Re: kaius711]
#23649948 - 09/16/16 09:11 AM (7 years, 6 months ago) |
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Yep exactly what we're saying....that's why we recommend agar work so much....it will save you time and money using agar....
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Leftfield420
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Registered: 02/26/16
Posts: 10,068
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Re: Assistance in using agar for pf tek [Re: kaius711]
#23649952 - 09/16/16 09:13 AM (7 years, 6 months ago) |
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Check em out....I don't live anywhere near Australia
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kaius711
Wandering Entity
Registered: 09/15/16
Posts: 63
Last seen: 6 years, 1 month
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Re: Assistance in using agar for pf tek [Re: amidogen]
#23649954 - 09/16/16 09:14 AM (7 years, 6 months ago) |
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i shall hunt some more then.
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Leftfield420
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Registered: 02/26/16
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Re: Assistance in using agar for pf tek [Re: kaius711]
#23649967 - 09/16/16 09:21 AM (7 years, 6 months ago) |
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I did pf tek 1 time.....ran 6 cakes and got about 21g's dry...do a couple runs in and research going bulk.....IMO it's a lot of work and your dehydrator is gonna be on constantly harvesting trying to dry cakes a few at a time....because they will mature at different times...kinda a pain in the ass but worth it
Edited by Leftfield420 (09/16/16 09:22 AM)
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NumeroEno
I come from the land of lizards
Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Assistance in using agar for pf tek [Re: Leftfield420]
#23649971 - 09/16/16 09:26 AM (7 years, 6 months ago) |
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Yeah PF tek is a good way to do your first grow, but once you get the concepts down bulk is where it's at.
-------------------- Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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