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OfflinePinpapa
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Knots in my L.c.
    #23648552 - 09/15/16 07:29 PM (7 years, 4 months ago)

I'm getting what looks like knots and little pinheads in a liquid culture that's a week old clone off a plate on witch little pins were growing off a tissue sample.This plate has been sitting around since 2014.I decided to give myco engineering another go.So these knots are they aggressive mycelium or bacteria ?  I have knocked a few jars @ Day three with nada growth.The first pic is the myc. at rest on the bottom.The second pic is spinning in the jar.


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OfflineMushierage
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Re: Knots in my L.c. [Re: Pinpapa]
    #23648590 - 09/15/16 07:38 PM (7 years, 4 months ago)

Bin it unless you started with clean agar.


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



Edited by Mushierage (09/15/16 08:13 PM)


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OfflinePinpapa
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Re: Knots in my L.c. [Re: Mushierage]
    #23648616 - 09/15/16 07:47 PM (7 years, 4 months ago)

Then by all means I better trash it, although I explained in the post that it is cloned tissue. Your advise would be a bit more valuable if you read the post first.


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OfflineGreg
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Re: Knots in my L.c. [Re: Pinpapa]
    #23648623 - 09/15/16 07:49 PM (7 years, 4 months ago)

If you can work with agar you should just put a drop on another plate.
If it's bacterial it will be pretty obvious.

I've had bacteria show noticeable growth after 18 hours on agar.


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OfflinePinpapa
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Re: Knots in my L.c. [Re: Greg]
    #23648641 - 09/15/16 07:54 PM (7 years, 4 months ago)

I'm just wondering if any one else has seen this phenomenon (knots and pins ) in a L.c..I'll know if it's contaminated soon enuough.


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Edited by Pinpapa (09/15/16 07:57 PM)


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OfflineGreg
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Re: Knots in my L.c. [Re: Pinpapa]
    #23648649 - 09/15/16 07:55 PM (7 years, 4 months ago)

I've had similar stuff before. Usually from myc forming around small bits of sediment.

Hard to say if that's what's going on though.


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OfflineMushierage
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Re: Knots in my L.c. [Re: Pinpapa]
    #23648652 - 09/15/16 07:55 PM (7 years, 4 months ago)

I must've misread, because I could have sworn it just said your pins were growing off the tissue sample grown in the LC. Don't think that's happening, but I digress. 

Perhaps add a little more detail in there because from what I read, I got that you didn't use agar at all. 

AT ANY RATE, all this is really pointless, because at the end of the post, I did say bin it unless you started off with clean agar.  If you did, that's great.  Grow away.  If it wasn't clean, again, your LC is trash.


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflinePinpapa
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Re: Knots in my L.c. [Re: Mushierage]
    #23648667 - 09/15/16 08:00 PM (7 years, 4 months ago)

It started on a 2 plus year old plate!I removed a couple pins that grew on the plate and tossed them in L.c.


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OfflineMushierage
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Re: Knots in my L.c. [Re: Pinpapa]
    #23648679 - 09/15/16 08:04 PM (7 years, 4 months ago)

I understand now bub.  I did misread, apologies. Small phones suck.  I've never encounter pins in an LC ever however.  If you're confident it's clean, knock a jar with it and see what it does.  That's the thing about LCs.  You never really can tell until you test it. 


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineMushierage
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Re: Knots in my L.c. [Re: Mushierage]
    #23648691 - 09/15/16 08:08 PM (7 years, 4 months ago)

I will say this though.  If you had this on a plate for two years, and not a slant, even refrigerated, bacteria can proliferate.  This is why people store their cultures in slants for years, and not plates.


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineX2QE
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Re: Knots in my L.c. [Re: Mushierage]
    #23648720 - 09/15/16 08:19 PM (7 years, 4 months ago)

Why does a plate contaminate but not a slant? I thought the only reason for slants was they take much longer to dry out.


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OfflineMushierage
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Re: Knots in my L.c. [Re: X2QE]
    #23648736 - 09/15/16 08:22 PM (7 years, 4 months ago)

Because plates usually already have bacteria on them to some degree or another.  When slants are started, you generally do them with super clean cultures and the surface area is so small, the culture doesn't give bacteria a chance to get on the agar. 

And yes, they do take much longer to dry out.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflinePinpapa
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Re: Knots in my L.c. [Re: X2QE]
    #23648750 - 09/15/16 08:31 PM (7 years, 4 months ago)

Recently mushrooms have lured me back. I live on a 100 acres and I want to learn how to remediate contaminated soils as a business.So while I am waiting for my cubes to arrive for honing my skills I found this old plate that still looked OK for it's age, and I thought why not try to revive it.


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OfflinePsilocyBen17
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Re: Knots in my L.c. [Re: Mushierage]
    #23648759 - 09/15/16 08:35 PM (7 years, 4 months ago)

Quote:

Mushierage said:
Bin it unless you started with clean agar.





I won't use a plate for anything if its been colonized for more than a week or two....

I doubt a plate from 2014 would be viable....I suspect all you are seeing in that jar is bacteria. If anything you should drop a piece of the old agar on fresh plate and use new growth as soon as you see enough to transfer to the LC

Can i ask how the plate was stored? Do you have a pic?


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OfflineMushierage
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Re: Knots in my L.c. [Re: Pinpapa]
    #23648760 - 09/15/16 08:36 PM (7 years, 4 months ago)

Yeah I hear ya.  I just say make up a test jar a knock it up.  See what it does.  If it goes bacterial, it goes bacterial.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflinePsilocyBen17
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Re: Knots in my L.c. [Re: Mushierage]
    #23648790 - 09/15/16 08:52 PM (7 years, 4 months ago)

Yeah or make an agar plate if you have the means (and everyone has the means)....better IMO to waste agar than grains.


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OfflinePinpapa
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Re: Knots in my L.c. [Re: PsilocyBen17]
    #23649485 - 09/16/16 03:42 AM (7 years, 4 months ago)

Plate was stored on a shelf , sealed  with tape . At the center of the plate there was a blob of pins  witch I removed, dropped in L.c.and then hit with the blender.No pics taken.


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