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lovelaughlibs
Dopamine Slave



Registered: 10/14/15
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Agar germination times? 1
#23647006 - 09/15/16 09:55 AM (7 years, 4 months ago) |
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I made some pasty plates and let them cool to room temperature then inoculated them in a SAB with a single drop of spore solution from a sterilised needle. How long will it be until I see growth on the plates?
Also, I tried the same thing last time but I did not let the plates cool for long enough, would the overly warm temperature of the agar explain why I saw nothing, not even contams after a week?
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mrmazdarx9
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Registered: 05/15/16
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It differs you should see something within a week but my PEU plate took two weeks to show anything
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lovelaughlibs
Dopamine Slave



Registered: 10/14/15
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Re: Agar germination times? [Re: mrmazdarx9]
#23647027 - 09/15/16 10:03 AM (7 years, 4 months ago) |
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If it helps at all it was a B+ MS syringe
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mrmazdarx9
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Registered: 05/15/16
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Won't make much of a difference give them ambient light and room temp that's how I keep mine. They sit in my coat cupboard with the doors open so the light through the front door can get to them
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Agar germination times? [Re: mrmazdarx9]
#23647033 - 09/15/16 10:07 AM (7 years, 4 months ago) |
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Quote:
mrmazdarx9 said: It differs you should see something within a week but my PEU plate took two weeks to show anything
I've had a tamp culture take 2 months to germinate lol.
Highly doubt the agar being warmish stopped germination, since spores easily survive a hot pour
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mrmazdarx9
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Re: Agar germination times? [Re: Mad Season]
#23647040 - 09/15/16 10:09 AM (7 years, 4 months ago) |
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That makes me feel better these PEU plates all show nothing for ages, did 3 more to see if it was a one off on 6th September and up to now nothing
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mushpunx
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Registered: 04/20/14
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Re: Agar germination times? [Re: mrmazdarx9] 1
#23647166 - 09/15/16 11:05 AM (7 years, 4 months ago) |
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If you have spores that really dont wanna germinate on agar (even after recipe changes) you can inoculate a small jar of grain and onve its colonized for a grain with a sterile tool and start a culture from that
Next time don't drop spores onto the agar- if you are using a spore syringe drop it onto a sterile cotton swab and swipe across the plate in a zig zag pattern like you would with a dry print and an inoculation loop.
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mrmazdarx9
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Re: Agar germination times? [Re: mushpunx]
#23647172 - 09/15/16 11:08 AM (7 years, 4 months ago) |
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I'll give that a go next time
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