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Offlinelovelaughlibs
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Agar germination times? * 1
    #23647006 - 09/15/16 09:55 AM (7 years, 4 months ago)

I made some pasty plates and let them cool to room temperature then inoculated them in a SAB with a single drop of spore solution from a sterilised needle. How long will it be until I see growth on the plates?

Also, I tried the same thing last time but I did not let the plates cool for long enough, would the overly warm temperature of the agar explain why I saw nothing, not even contams after a week?


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Offlinemrmazdarx9
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Re: Agar germination times? [Re: lovelaughlibs]
    #23647013 - 09/15/16 09:59 AM (7 years, 4 months ago)

It differs you should see something within a week but my PEU plate took two weeks to show anything


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Re: Agar germination times? [Re: mrmazdarx9]
    #23647027 - 09/15/16 10:03 AM (7 years, 4 months ago)

If it helps at all it was a B+ MS syringe :smile:


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Re: Agar germination times? [Re: lovelaughlibs]
    #23647030 - 09/15/16 10:06 AM (7 years, 4 months ago)

Won't make much of a difference give them ambient light and room temp that's how I keep mine. They sit in my coat cupboard with the doors open so the light through the front door can get to them


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Re: Agar germination times? [Re: mrmazdarx9]
    #23647033 - 09/15/16 10:07 AM (7 years, 4 months ago)

Quote:

mrmazdarx9 said:
It differs you should see something within a week but my PEU plate took two weeks to show anything



I've had a tamp culture take 2 months to germinate lol.

Highly doubt the agar being warmish stopped germination, since spores easily survive a hot pour


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Re: Agar germination times? [Re: Mad Season]
    #23647040 - 09/15/16 10:09 AM (7 years, 4 months ago)

That makes me feel better these PEU plates all show nothing for ages, did 3 more to see if it was a one off on 6th September and up to now nothing


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Offlinemushpunx
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Re: Agar germination times? [Re: mrmazdarx9] * 1
    #23647166 - 09/15/16 11:05 AM (7 years, 4 months ago)

If you have spores that really dont wanna germinate on agar (even after recipe changes) you can inoculate a small jar of grain and onve its colonized  for a grain with a sterile tool and start a culture from that


Next time don't drop spores onto the agar- if you  are using a spore syringe drop it onto a sterile cotton swab and swipe across the plate in a zig zag pattern like you would with a dry print and an inoculation loop.


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Re: Agar germination times? [Re: mushpunx]
    #23647172 - 09/15/16 11:08 AM (7 years, 4 months ago)

I'll give that a go next time:thumbup:


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