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Offlinekaius711
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Liquid culture first time questions
    #23646926 - 09/15/16 09:17 AM (7 years, 4 months ago)

Greetings. I have never grown shrooms before and am trying gain as much knowledge as possible before i start so please bear with me. Firstly, i have seen many mixed opinions about liquid culture in terms of success so my questions is how to maximise success rate and also can you create LC from LC? I thank you for your assistance in advance.


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Offlinemrmazdarx9
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Re: Liquid culture first time questions [Re: kaius711]
    #23646930 - 09/15/16 09:19 AM (7 years, 4 months ago)

What equipment do you have to start with. Sterility is one of the most important factors


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OfflineYaMoonSun
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Re: Liquid culture first time questions [Re: mrmazdarx9]
    #23646936 - 09/15/16 09:23 AM (7 years, 4 months ago)

Those silicon injection ports look promising for LC to LC transfers. Have yet to try one out yet though.


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Offlineblueconfusion
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Re: Liquid culture first time questions [Re: mrmazdarx9]
    #23646942 - 09/15/16 09:27 AM (7 years, 4 months ago)

If you want to do an LC start with agar, get a clean culture and move from there.  Personally I do not and will not use LCs, I make clean agar plates, by the 3rd transfer I usually am confident I have a good clean plate.  Then I make grain masters, and from those grain masters i set to work.  You can always make fresh cultures or make more masters from a good producing set of jars.


--------------------


Edited by blueconfusion (09/15/16 09:28 AM)


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Offlinekaius711
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Re: Liquid culture first time questions [Re: blueconfusion]
    #23646962 - 09/15/16 09:36 AM (7 years, 4 months ago)

I am not familiar at all with agar plates. Having purchased a 10ml spore syringe i simply want to create a simple system that does not requre much equipment and will give me infinite spores so that i do not have to buy more syringes to inoculate cakes.


Edited by kaius711 (09/15/16 09:39 AM)


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Offlinemrmazdarx9
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Re: Liquid culture first time questions [Re: kaius711]
    #23646965 - 09/15/16 09:38 AM (7 years, 4 months ago)

Then agar is your friend
Pasty's teks are about as noon friendly as you get
https://www.shroomery.org/forums/showflat.php/Number/19208976


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Offlinekaius711
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Re: Liquid culture first time questions [Re: mrmazdarx9]
    #23646971 - 09/15/16 09:41 AM (7 years, 4 months ago)

It what ways will it provide an indefinite amount of spores?


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OfflineYaMoonSun
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Re: Liquid culture first time questions [Re: kaius711]
    #23646975 - 09/15/16 09:42 AM (7 years, 4 months ago)

I agree with the agar comment whole heartedly

Taking good prints is harder than it sounds. I just recently did my first clean spore syringe. Had to find a PP5 container and toss some aluminum foil squares inside, and then pressure cooked it for like 30 minutes at 15psi and placed into my SAB until I had an good sized cap for printing.


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Offlinemrmazdarx9
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Re: Liquid culture first time questions [Re: kaius711]
    #23646982 - 09/15/16 09:46 AM (7 years, 4 months ago)

One drop of spore solution onto agar can then be transfered many times to get the cleanest best plate possible and you can take a slice of that plate grow it in grains or make an LC or whatever you want, grow out some shrroms take a print, swab back onto agar start again, with prints you only need a tiny speck of spores onto agar. So a print lasts ages and you can make multiple grows from that speck


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Offlinemrmazdarx9
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Re: Liquid culture first time questions [Re: YaMoonSun]
    #23646984 - 09/15/16 09:47 AM (7 years, 4 months ago)

Quote:

YaMoonSun said:
I agree with the agar comment whole heartedly

Taking good prints is harder than it sounds. I just recently did my first clean spore syringe. Had to find a PP5 container and toss some aluminum foil squares inside, and then pressure cooked it for like 30 minutes at 15psi and placed into my SAB until I had an good sized cap for printing.



I never bother with sterilizing my foil, the cap ain't sterile anyway, just clean up on agar.


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Offlinekaius711
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Re: Liquid culture first time questions [Re: mrmazdarx9]
    #23646989 - 09/15/16 09:49 AM (7 years, 4 months ago)

I shall research agar extensively then. Your assistance is greatly appreciated.


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Offlinemrmazdarx9
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Re: Liquid culture first time questions [Re: kaius711]
    #23646999 - 09/15/16 09:52 AM (7 years, 4 months ago)

Quote:

kaius711 said:
I shall research agar extensively then. Your assistance is greatly appreciated.



Its the best place to start your shroom journey, wish I had of started with agar I'd be so much better at it by now.


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InvisiblePastywhyteMDiscord
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Re: Liquid culture first time questions [Re: mrmazdarx9] * 1
    #23647020 - 09/15/16 10:02 AM (7 years, 4 months ago)

Agar is super easy. Take 5 plates, put down a drop on each of them. Transfer and pick the best looking one. Make a master, G2G for 3 expansions. Should take a few weeks. Take the 1000 quarts of spawn you just made and make 200 monotubs. Take 30-50 clones from those, chose the best of them all and have a killer culture to grow forever. Meanwhile throw the 100 lbs of mushrooms you just grew in a bonfire and start growing some more.

Put spore syringe that you used 5 drops out of in a drawer and never look at it again.




Or just fire the syringe into a half gallon of sterile water if you don't want to do agar and just want to stretch the spores. Will still be more than enough.


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Offlinemrmazdarx9
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Re: Liquid culture first time questions [Re: Pastywhyte]
    #23647053 - 09/15/16 10:13 AM (7 years, 4 months ago)

Quote:

Pastywhyte said:
Agar is super easy. Take 5 plates, put down a drop on each of them. Transfer and pick the best looking one. Make a master, G2G for 3 expansions. Should take a few weeks. Take the 1000 quarts of spawn you just made and make 200 monotubs. Take 30-50 clones from those, chose the best of them all and have a killer culture to grow forever. Meanwhile throw the 100 lbs of mushrooms you just grew in a bonfire and start growing some more.

Put spore syringe that you used 5 drops out of in a drawer and never look at it again.




Or just fire the syringe into a half gallon of sterile water if you don't want to do agar and just want to stretch the spores. Will still be more than enough.



:rofl::whathesaid:


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Invisible36fuckin5
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Re: Liquid culture first time questions [Re: mrmazdarx9]
    #23647085 - 09/15/16 10:27 AM (7 years, 4 months ago)

I always liked to take an inoculation loop and squirt a drop of spore solution on that then inoculate with the loop. Prevents the solution from getting all over your plate.


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bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Invisiblemorty422
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Re: Liquid culture first time questions [Re: 36fuckin5]
    #23647093 - 09/15/16 10:32 AM (7 years, 4 months ago)

Quote:

36fuckin5 said:
I always liked to take an inoculation loop and squirt a drop of spore solution on that then inoculate with the loop. Prevents the solution from getting all over your plate.




Instructions un-clear.....filled PP5 with spore solution...

Jk

I also like to swipe the drop with a loop. It kept my growth centered and was more noob friendly.


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Offlinekaius711
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Re: Liquid culture first time questions [Re: Pastywhyte]
    #23648484 - 09/15/16 07:09 PM (7 years, 4 months ago)

200 monotubs! Godamn thats way too much i aim only to cultivate around 50 grams dried per month


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InvisiblePastywhyteMDiscord
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Re: Liquid culture first time questions [Re: kaius711]
    #23648509 - 09/15/16 07:18 PM (7 years, 4 months ago)

Quote:

kaius711 said:
200 monotubs! Godamn thats way too much i aim only to cultivate around 50 grams dried per month




Then the last thing you need to mess with is liquid culture. Cause LC is used to make 100's of quarts of spawn fast. If you just wanna run a single mini or something LC would be a whole lot of risk for no reason.


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OfflineMushierage
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Re: Liquid culture first time questions [Re: Pastywhyte]
    #23648532 - 09/15/16 07:24 PM (7 years, 4 months ago)

A gallon LC?  :beardstroke:


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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InvisibleInocuole
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Re: Liquid culture first time questions [Re: 36fuckin5]
    #23648542 - 09/15/16 07:27 PM (7 years, 4 months ago)

Quote:

36fuckin5 said:
I always liked to take an inoculation loop and squirt a drop of spore solution on that then inoculate with the loop. Prevents the solution from getting all over your plate.




I do the same with swabs. :shrug:


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Invisible36fuckin5
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Re: Liquid culture first time questions [Re: Mushierage]
    #23648548 - 09/15/16 07:28 PM (7 years, 4 months ago)

Quote:

Mushierage said:
A gallon LC?  :beardstroke:




Unless you have a team of people working for you (and you shouldn't) then it'd go bad before you got to it all.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineMushierage
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Re: Liquid culture first time questions [Re: 36fuckin5]
    #23648576 - 09/15/16 07:35 PM (7 years, 4 months ago)

It was more a joke.  I'm looking to expand my jars but not that damn much lol.  Maybe about 50 jars would be alright and for that I'll just stick with g2g as I already have several healthily colonized quarts of oat spawn.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Invisible36fuckin5
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Re: Liquid culture first time questions [Re: Mushierage]
    #23648581 - 09/15/16 07:36 PM (7 years, 4 months ago)

5 quarts of spawn can easily make 50.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineMushierage
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Re: Liquid culture first time questions [Re: 36fuckin5]
    #23648599 - 09/15/16 07:40 PM (7 years, 4 months ago)

Yes.  I have 3 grain masters at the moment that will be used for g2g.  If I need to expand from there it'll probably be from a new culture as I have some aa+ spores coming my way.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Invisible36fuckin5
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Re: Liquid culture first time questions [Re: Mushierage]
    #23648819 - 09/15/16 09:09 PM (7 years, 4 months ago)

You can g2g those again for more spawn. No need to wait for spores. 3 generations is the max that most reccomend. I'm with them, though I have occasionally gotten good results pushing it farther.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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InvisiblePastywhyteMDiscord
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Re: Liquid culture first time questions [Re: 36fuckin5]
    #23648826 - 09/15/16 09:12 PM (7 years, 4 months ago)

I have pushed loads of P values. But past 3 g2g you run the risk of the original grain becoming bacterial which is a major mold vector. 3 g2g from a quart could be turned into 100 pretty easy. No reason to go past 3 really.


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OfflineThedillestpickle
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Re: Liquid culture first time questions [Re: Pastywhyte]
    #23648886 - 09/15/16 09:48 PM (7 years, 4 months ago)

not sure if OP caught all that...

You don't really want more spores. What you want is a method of taking a small amount of spores and expanding mycelial growth so that you have lots of spawn.

To do that you have two routes:
a)start with agar and transfer clean growth to jars to make grain master which will be G2G'd to other jars to produce lots of spawn
b)start with agar and transfer to LC broth to be grown out and used to inoculate lots of spawn.

If you want to scale up you can always produce:
a)more grain masters to g2g to more spawn jars
b)create a larger LC to inoculate more spawn jars


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Offlinekaius711
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Re: Liquid culture first time questions [Re: Pastywhyte]
    #23649221 - 09/16/16 12:12 AM (7 years, 4 months ago)

i see. then agar it is.


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InvisibleInocuole
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Re: Liquid culture first time questions [Re: kaius711]
    #23649233 - 09/16/16 12:18 AM (7 years, 4 months ago)

:thumbup:

It's everything good about LC without the major drawbacks.


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Invisible36fuckin5
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Re: Liquid culture first time questions [Re: Inocuole]
    #23650479 - 09/16/16 01:12 PM (7 years, 4 months ago)

Quote:

Inocuole said:
:thumbup:

It's everything good about LC without the major drawbacks.




And a lot more advantages.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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