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Offlinetump
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Registered: 03/17/16
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Re: Long time, no see, Shroomery... What's hype nowadays? I need critique! [Re: twistedty]
    #23646647 - 09/15/16 06:01 AM (7 years, 4 months ago)

Making agar is like making jello then pressure cooking it sterile. On the other hand bacterial things happen with all open air contact. I use agar more then i like to tell on here. But he asked about spore syringes i just answered him. With semi clean spores to grain it takes time but you still get more for your money if you take a 6 dallor investment and make it into a 1 dallor investment for 10 jars.
Like i say agar is a skill set and im not the only one for fails at it. Either you i get 75% fast colonised clean grains with agar. So 95% clean but slow colonised grains.
Agar has its place . It's not a holy grail its just a tool. It is not even the same crappy arguement the tc have with the noobs on here everyday.
Ive done agar. I have agar plates going right now. But i suck at them and probaby will umtil i die. Every step we do to in the mushroom life cycle increases the chances of contams winning. Every agar transfer is another area for contams to sneak in. Atleast if you add more clean water and divide the spore load between more jars the other mold spores will be deluded. With flame sterilition and proper clean injections are much better off.


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Offlinespacechildo
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Re: Long time, no see, Shroomery... What's hype nowadays? I need critique! [Re: tump]
    #23646695 - 09/15/16 06:54 AM (7 years, 4 months ago)

:facepalm3:


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Invisiblefilthyknees
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Registered: 03/08/13
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Re: Long time, no see, Shroomery... What's hype nowadays? I need critique! [Re: spacechildo]
    #23646715 - 09/15/16 07:10 AM (7 years, 4 months ago)

I would most likely never use diluted spore syringes over agar.

I would say the best advice there is to grow is to use agar.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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Offlinetump
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Re: Long time, no see, Shroomery... What's hype nowadays? I need critique! [Re: filthyknees]
    #23646754 - 09/15/16 07:36 AM (7 years, 4 months ago)

Again you all treat agar as a holy grail. I answer him with my personal history on the subject.


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Invisiblefilthyknees
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Re: Long time, no see, Shroomery... What's hype nowadays? I need critique! [Re: tump]
    #23646831 - 09/15/16 08:28 AM (7 years, 4 months ago)

So did i


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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OfflineTripBalls
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Re: Long time, no see, Shroomery... What's hype nowadays? I need critique! [Re: filthyknees]
    #23647521 - 09/15/16 01:32 PM (7 years, 4 months ago)

Can someone direct me to a good agar tek to start with,
And maybe a tek for a good SAB, or whatever is preferred nowadays... I don't like the idea of these new trashbag flow hood things. :awesome:


--------------------
I Followed Frank's Footsteps This guy is a hero.
Bod’s system saved my life as well.. if these guys can’t get you where you need to be, you’re in the wrong hobby.

Rate me cause I'm a nice guy? :hi:

Not all who wander are lost, but I sure as fuck am
:mushroom2:


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InvisibleBoogieman47
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Re: Long time, no see, Shroomery... What's hype nowadays? I need critique! [Re: TripBalls]
    #23647526 - 09/15/16 01:33 PM (7 years, 4 months ago)

Pastywhytes easy agar tek


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InvisibleInocuole
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Re: Long time, no see, Shroomery... What's hype nowadays? I need critique! [Re: Boogieman47]
    #23647698 - 09/15/16 02:38 PM (7 years, 4 months ago)

Tump just... stop posting.  Seriously.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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OfflineTripBalls
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Re: Long time, no see, Shroomery... What's hype nowadays? I need critique! [Re: Inocuole]
    #23648131 - 09/15/16 05:11 PM (7 years, 4 months ago)

That agar tek is gnarly.
I can't wait to try it..
Especially given most the shit I can get locally,
But can I do a no-pour using a PDA mix from a sponsor in place of the agar-agar?

And As for the slurry, does an oster blender fit onto a mason or do I have to modify a lid to fit the blade?
I'm about to use up one of my syringes on the 7 jars I re-cooked yesterday..

Should I add cuffs and caps to my SAB?
I overkill on sterility and still wind up getting shit jars.
Lysol spray everywhere, and soapy water sprayed on the walls of the SAB.

Thanks all! I can't wait to see some rhizo.


--------------------
I Followed Frank's Footsteps This guy is a hero.
Bod’s system saved my life as well.. if these guys can’t get you where you need to be, you’re in the wrong hobby.

Rate me cause I'm a nice guy? :hi:

Not all who wander are lost, but I sure as fuck am
:mushroom2:


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Offlinespacechildo
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Re: Long time, no see, Shroomery... What's hype nowadays? I need critique! [Re: TripBalls]
    #23648191 - 09/15/16 05:30 PM (7 years, 4 months ago)

how to use a SAB, a piece from SBJs "the basics" https://www.shroomery.org/forums/showflat.php/Number/20048771#20048771
checkout the rest of his stuff!
its all about still air, not sterile air(which is impossible)

go for clean, fast, healthy growth instead of "rhizo".
stro's cleaning and isolating on agar https://www.shroomery.org/forums/showflat.php/Number/18430998/fpart/1/vc/1/nt/4

IMO you should clone a fruit from a tub, or let a (clean) pasty agar plate fully colonize and clone an invitro pin from the plate. they're usually pretty good.


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OfflineTripBalls
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Re: Long time, no see, Shroomery... What's hype nowadays? I need critique! [Re: spacechildo]
    #23648490 - 09/15/16 07:13 PM (7 years, 4 months ago)

Quote:

spacechildo said:


go for clean, fast, healthy growth instead of "rhizo".


IMO you should clone a fruit from a tub, or let a (clean) pasty agar plate fully colonize and clone an invitro pin from the plate. they're usually pretty good.





I'm sorry, I'm so infatuated with the way mycelium grows.. Rhizomorphic growth makes me feel rewarded... Whether or not it has any bearing on how well it actually was grown..


And speaking of cloning fruits, is it worth it to inoculate my plates with spore solution?
I will likely create a master and do G2G to get my supply bulked up, and use a little bit in a slurry...
But the slurry part is what gets me, I feel like it is entirely too much room for error

We'll see how my spawn goes.. I feel like these spores have been abused.


--------------------
I Followed Frank's Footsteps This guy is a hero.
Bod’s system saved my life as well.. if these guys can’t get you where you need to be, you’re in the wrong hobby.

Rate me cause I'm a nice guy? :hi:

Not all who wander are lost, but I sure as fuck am
:mushroom2:


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InvisibleInocuole
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Re: Long time, no see, Shroomery... What's hype nowadays? I need critique! [Re: TripBalls]
    #23648522 - 09/15/16 07:23 PM (7 years, 4 months ago)

Well, as long as you know you're liable to pass up good genetics by only looking for rhizomorphs..


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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