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chas5511
Stranger
Registered: 09/15/15
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Last seen: 5 years, 5 months
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Rye grain inoculation question
#23645279 - 09/14/16 05:09 PM (7 years, 4 months ago) |
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Hello there. Recently I knocked my first quart grain jar ever. I am using a jar with a self healing injection port and a filter at the top. My question is this....
I tilted the jar on its side so the syringe would actually push into the grains. I then slowly tilted the jar right side up. After doing some reading I found this may be slowing my colonization time. Its been 4 days and I haven't seen any growth. Should I reinoculate the jar but this time leave it right side up and drop the spores on top of the grain? This would avoid splitting the spores apart. Thanks for any input.
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Jake the Cake


Registered: 07/24/16
Posts: 80
Loc: Under a rock
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Re: Rye grain inoculation question [Re: chas5511]
#23645377 - 09/14/16 05:54 PM (7 years, 4 months ago) |
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Relax. 4 days is nothing and no. do not re inoculate. Wait a few weeks and see if anything happens
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Smoothcat
Renegade-master



Registered: 10/07/15
Posts: 1,254
Last seen: 5 months, 28 days
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Spores can take a long time to germinate, relax, the magic will happen if your grain is prepped correctly.
If you really want to see the magic, you need agar.
Good luck and get some pics up
-------------------- Back once again with the ill behaviour Links I like
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Mushierage
SWIM Sinker



Registered: 06/29/16
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Re: Rye grain inoculation question [Re: Smoothcat]
#23645451 - 09/14/16 06:31 PM (7 years, 4 months ago) |
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Spores do sometimes take awhile to germinate, but I believe that the length of time it takes it highly dependent on how well your grain was prepped, and conditions. If it's prepped extremely well, and you're keeping your jars at proper temps, with proper GE and ambient light, it can take 4-5 days. Sometimes less if you're damn lucky but I've done a lot of MS to grain and generally for me, this is what I expect.
I've moved to better things since then however.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Rye grain inoculation question [Re: chas5511]
#23645522 - 09/14/16 06:54 PM (7 years, 4 months ago) |
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Quote:
chas5511 said: I tilted the jar on its side so the syringe would actually push into the grains.
How full is this jar?
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chas5511
Stranger
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Re: Rye grain inoculation question [Re: Pastywhyte]
#23645576 - 09/14/16 07:12 PM (7 years, 4 months ago) |
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Thanks for the replys all. The jar is just over 3/4 full. The syringe wont quite reach the grain if the jar is sitting regularly.
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Pastywhyte
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Registered: 09/15/12
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Re: Rye grain inoculation question [Re: chas5511]
#23645581 - 09/14/16 07:13 PM (7 years, 4 months ago) |
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You don't need the syringe to reach the grain. Just a few drops on top will do the trick.
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chas5511
Stranger
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Re: Rye grain inoculation question [Re: Pastywhyte]
#23645590 - 09/14/16 07:17 PM (7 years, 4 months ago) |
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Okay thanks. So i should just leave it alone rather than put some more drops on the top?
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Pastywhyte
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Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Rye grain inoculation question [Re: chas5511]
#23645603 - 09/14/16 07:24 PM (7 years, 4 months ago) |
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Just leave it.
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chas5511
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Re: Rye grain inoculation question [Re: Pastywhyte]
#23645620 - 09/14/16 07:32 PM (7 years, 4 months ago) |
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chas5511
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Re: Rye grain inoculation question [Re: chas5511]
#23645627 - 09/14/16 07:35 PM (7 years, 4 months ago) |
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Also is 1cc for a quart enough inoculate?
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Pastywhyte
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Registered: 09/15/12
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Re: Rye grain inoculation question [Re: chas5511]
#23645662 - 09/14/16 07:50 PM (7 years, 4 months ago) |
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Way more than enough.
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chas5511
Stranger
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Re: Rye grain inoculation question [Re: Pastywhyte]
#23651664 - 09/16/16 09:58 PM (7 years, 4 months ago) |
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Now still no sign of growth but slight condensation at the top of the jar. Is this normal?
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Mushierage
SWIM Sinker



Registered: 06/29/16
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Re: Rye grain inoculation question [Re: chas5511]
#23651700 - 09/16/16 10:13 PM (7 years, 4 months ago) |
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Yes. A little condensation at the top of the jar is pretty normal. But a bunch around the grain itself isn't. Causes bacteria to proliferate, and spores have a hard time germinating in such conditions, as previously mentioned.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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chas5511
Stranger
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Last seen: 5 years, 5 months
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Re: Rye grain inoculation question [Re: Mushierage]
#23668134 - 09/22/16 01:40 PM (7 years, 4 months ago) |
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12 days later and still no growth. Should i be worried?
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enlightenment
alchemist



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Re: Rye grain inoculation question [Re: chas5511]
#23668193 - 09/22/16 01:59 PM (7 years, 4 months ago) |
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Whats the room temperature?
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chas5511
Stranger
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75 pretty steadily.
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enlightenment
alchemist


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Re: Rye grain inoculation question [Re: chas5511]
#23668232 - 09/22/16 02:11 PM (7 years, 4 months ago) |
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OK. Temperature is nice. After 12 days in most cases you should see something. I had 4 year old spores that took more than 20 days to germinate on agar. Where did you get your syringe from? From a shroomery sponsor? Is there a 'spore clump' in the middle of the syringe? If there is one: Did you shake the syringe to dissolve the clump before inoculation?
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chas5511
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Last seen: 5 years, 5 months
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I did indeed shake and i did in fact get the spores from a shroomery sponsor. I bought my rye grain jar from a well trusted site as well. Could it be due to the fact that i tilted the jar and shifted the grains around slightly during inoc? The jar has a self healing port and the syringe wouldnt reach the grain so i tilted the grain towards the injection port. I should have just dripped it on the top. Perhaps i should reinoculate and keep the spores together?
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enlightenment
alchemist


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Posts: 1,647
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Re: Rye grain inoculation question [Re: chas5511]
#23668269 - 09/22/16 02:22 PM (7 years, 4 months ago) |
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Every drop of water from the syringe should contain enough spores. You dripped in about 1cc that should be more than enough as Pastywhyte already wrote. Everything seems to be OK. I doubt that it has to do with shifting the grains. You could wait this weekend and inoculate again on Monday without moving the jar.
Patience is hard for a newcomer but it is really essential in this hobby.
I wish you luck and success. Don't get disappointed. Keep us posted
Edited by enlightenment (09/22/16 02:28 PM)
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