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DeadManTrips
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Registered: 04/22/15
Posts: 55
Last seen: 3 years, 1 month
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Peculiar growth from bulk substrate p. cubensis
#23643788 - 09/14/16 04:17 AM (7 years, 4 months ago) |
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Hey everyone,
I've had a few tubs going these past couple of weeks. I just began FAE about 9 days ago. The tubs look fully colonised and normal. However, just today I had a look inside and found these interesting growths coming up from the substrate. I've seen this once before when I was growing some lions mane, but never have I encountered this with p. cubensis.
It's only appeared in 2 out of 4 tubs, which all were set up at the same time, each from a seperate bag of spawn.
I pulled a couple of them out to get a closer look.
I have a feeling that they are stressed or maybe over colonised or that there may not have been enough O2 circulating in the room for the first week?
Should I be concerned? Can anyone tell me what these are? I'm very interested to know.
See pics:

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tump
ban the undead



Registered: 03/17/16
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Re: Peculiar growth from bulk substrate p. cubensis [Re: DeadManTrips]
#23643814 - 09/14/16 04:48 AM (7 years, 4 months ago) |
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Those look like other mushrooms
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foragedfungus



Registered: 09/30/13
Posts: 1,849
Loc: out there
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Re: Peculiar growth from bulk substrate p. cubensis [Re: tump]
#23643821 - 09/14/16 04:52 AM (7 years, 4 months ago) |
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Looks like Hericium (lion's mane) to me. How did you make your spawn?
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Psilosoulful

Registered: 09/05/14
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Re: Peculiar growth from bulk substrate p. cubensis [Re: tump]
#23643850 - 09/14/16 05:27 AM (7 years, 4 months ago) |
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Quote:
tump said: Those look like other mushrooms
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DeadManTrips
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Registered: 04/22/15
Posts: 55
Last seen: 3 years, 1 month
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Re: Peculiar growth from bulk substrate p. cubensis [Re: foragedfungus]
#23643925 - 09/14/16 06:43 AM (7 years, 4 months ago) |
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The same way I always do. I've used fresh spore prints from the last batch to make clean spore syringes. Then injected the spores into bags filled with sterile grains. Following colonisation, I transferred the spawn to bulk sub.
This has really baffled me. I mean how the fuck did lions mane get in my cubes? haha. Shit.
There's literally no reasonable explanation for this that makes sense. The spores came directly from my last batch of cubes and the only time I've had lions mane in the house was almost a year ago and it was never anywhere near these tubs or bags.. Plus I never took any spores from the lions mane - it came to fruit and then I got rid of the remaining spawn..
I understand spores can last for a very long time, but I can't understand how they could've made it into either the prints or the syringe, let alone colonise alongside the cubensis spores...
Well, I guess if I don't see any pins sometime soon it looks as though hericium has won this time round... I'm going to be second guessing the remaining spore syringes in the fridge right now.
Any other suggestions?
-------------------- An adventurer, in search of his treasure...
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tump
ban the undead



Registered: 03/17/16
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Re: Peculiar growth from bulk substrate p. cubensis [Re: DeadManTrips]
#23644047 - 09/14/16 08:22 AM (7 years, 4 months ago) |
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Clone the loins mane its clearly a cut above the rest culture wise. Then trade it in market place for new cube prints. I have to know three things in this story. First how was the spore print taken step by step. Where was it taken in the house conpared to where you eat food. Third how you making spore syringes cleanly step by step. Do you have contams problems before. The reason i ask is i know that even venders on here have trouble sometimes keeping edibles spores out of cube prints. Don't get so down
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Peculiar growth from bulk substrate p. cubensis [Re: tump]
#23644067 - 09/14/16 08:37 AM (7 years, 4 months ago) |
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i think the story would be more like "lions mane as a species is a "cut above" cubensis in terms of how quick it fruits" not really that that strain of lions mane (which it may not even be) is a good one
I don't think I would spend time cloning it.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Peteyboy
SpaceWalker



Registered: 06/21/16
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Re: Peculiar growth from bulk substrate p. cubensis [Re: blindingleaf]
#23644074 - 09/14/16 08:40 AM (7 years, 4 months ago) |
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I'd listen to him
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foragedfungus



Registered: 09/30/13
Posts: 1,849
Loc: out there
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Re: Peculiar growth from bulk substrate p. cubensis [Re: tump]
#23644080 - 09/14/16 08:43 AM (7 years, 4 months ago) |
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The more I look at the pic, the more they looks like oysters that aren't getting any air.
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mrmazdarx9
Pffffttt


Registered: 05/15/16
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Re: Peculiar growth from bulk substrate p. cubensis [Re: foragedfungus]
#23644359 - 09/14/16 11:09 AM (7 years, 4 months ago) |
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Crazy stuff
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
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Re: Peculiar growth from bulk substrate p. cubensis [Re: mrmazdarx9]
#23644384 - 09/14/16 11:21 AM (7 years, 4 months ago) |
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The strands look too small to be the true lions mane. Definitely a hericium of some sort.. not sure what one tho. Kinda jealous you got some by accident tbh lol
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DeadManTrips
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Registered: 04/22/15
Posts: 55
Last seen: 3 years, 1 month
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Re: Peculiar growth from bulk substrate p. cubensis [Re: tump]
#23645838 - 09/14/16 09:12 PM (7 years, 4 months ago) |
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Firstly, the spore prints were taken by following a tek I found these forums. I've used it about 4 or 5 times now and never have I had any contam issues. So basically, using clean gloves (cleaned with isopropyl) I removed several mushrooms from the tub. Then with a brand new, sterile scalpel blade, I cut the cap from the stem from each mushroom. Then, I took about 2 - 3 caps and placed them onto clean (isopropyl cleaned) sheets of aluminium foil. Left them under a clean glass, with the lip of the glass held up by a small piece of sterile gauze. 12 - 18 hours later, I removed the cup, folded the aluminium foil and placed them into ziplock bags in the fridge.
As for the syringes, I had fresh sterile syringes and needles plus sterile h2o solution - so there was no chance of contam at this stage..
Quote:
foragedfungus said: The more I look at the pic, the more they looks like oysters that aren't getting any air.
Thank you foragedfungus, this just reminded me that I had some oysters growing nearby around the same time that these prints may have been taken - and I did see these growths in a bag that was probably eager for some air...
I think I know what has happened now. These prints were taken when I was still new to working with spores.. I think that maybe, these cube prints have been exposed to airborne Pleurotus spores! I think this means all my prints are contaminated...
If this is the case - and the more I think about it, it makes sense - can anyone explain how the oysters spores have out competed the cubes? I mean, the spore prints were black when looking at them with the naked eye - of course, the white oyster spores could very well be in there.. But given that the cubes spores would have vastly outnumbered the oysters spores, how has the oysters even survived? Are they a "stronger" coloniser than the cubes??
Last few questions,
does anyone know if the cubes will fruit alongside the oysters?
and is it logical to assume that my other spore syringes and one other print (all taken at the same time) are also contaminated and therefore not worth using?
I may have just lost my last available spores But at least I have learnt a valuable lesson here...
-------------------- An adventurer, in search of his treasure...
Edited by DeadManTrips (09/14/16 09:36 PM)
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Peculiar growth from bulk substrate p. cubensis [Re: DeadManTrips]
#23645895 - 09/14/16 09:38 PM (7 years, 4 months ago) |
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Haha damn yeah that's definitely oyster pins
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DeadManTrips
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Registered: 04/22/15
Posts: 55
Last seen: 3 years, 1 month
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Re: Peculiar growth from bulk substrate p. cubensis [Re: Mad Season]
#23646036 - 09/14/16 10:25 PM (7 years, 4 months ago) |
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Yep, I think thats it...
I'm still unsure as to how this happened with this batch, as I have done a previous grow 3 months ago with the same spore prints - and they worked out fine.
I took spore prints from this previous successful batch, which was not around any other mushrooms, so I think that this last print will be okay. I'll try get them into some jars or bags later this week and hopefully, this all works out haha.
Either way, I'll leave the tubs for now and just see what happens. I may get a few super strong cubensis pins come up (unlikely) or I might just expose them to more air and at least get some gourmet oysters for all my troubles :p
Lessons have been learnt here today. Thanks for everyone's input.
-------------------- An adventurer, in search of his treasure...
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vikingleif
Lazy Stoner



Registered: 08/11/16
Posts: 188
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Re: Peculiar growth from bulk substrate p. cubensis [Re: DeadManTrips]
#23646537 - 09/15/16 04:09 AM (7 years, 4 months ago) |
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show us a picture of your tub  i would be currious to follow a battle between cube and oyster hehe
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DeadManTrips
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Registered: 04/22/15
Posts: 55
Last seen: 3 years, 1 month
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Re: Peculiar growth from bulk substrate p. cubensis [Re: vikingleif]
#23717847 - 10/07/16 09:18 PM (7 years, 3 months ago) |
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Quote:
vikingleif said: show us a picture of your tub  i would be currious to follow a battle between cube and oyster hehe
In a strange turn of events, oyster mushrooms out colonise and out fruit the cubes - but just when I thought it was a clear win, two cubes popped up to say hello. Check this post with pictures:
https://www.shroomery.org/forums/showflat.php/Number/23717842/vc/1#23717842
-------------------- An adventurer, in search of his treasure...
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