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Invisibleflyontoast
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Registered: 08/20/16
Posts: 258
Why too many generations loses vitality/vigor?
    #23643258 - 09/13/16 10:26 PM (7 years, 4 months ago)

I have read that you shouldn't expand mycelium further than G4 or G5 before it loses vigor, and that you also shouldn't take spore prints from a mushroom you've cultivated because it will lose traits from its "wild" origins.
The first part, the over expanding, I can get because it kind of makes intuitive sense. But the second half, not taking spores from your cultivated mushroom, kind of flies in the face of most breeding practices in both plants and animals. If I wanted a plant to "adapt" to it's growing conditions and nutrients, you want to save your own seed. Can someone explain why this doesn't work for mushrooms? Like, if I take a spore print and make a syringe, turn it into a grain master on rye, then expand it into sorghum, and then maybe again into sorghum (or another grain), and finally into a nutrient dense fruiting substrate, shouldn't the spores from subsequent generations/fruiting body get better and ever more productive, or at least resilient, in similar growing conditions and nutrient sources?
On a related note, what else qualifies as "taming" as wild mushroom?


--------------------
:mushroom: My trade list :mushroom:
Looking for strong terrestrial fruiters for an outdoor beds experiment:
Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors.
Also, any commercially viable Pleurotus, cold or hot strains.
Thanks for the Q&A, trades, and all the posters & teachers that have come before us  :skol:


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OfflineTravelAgency
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Re: Why too many generations loses vitality/vigor? [Re: flyontoast]
    #23643536 - 09/14/16 12:20 AM (7 years, 4 months ago)

The spore part I've never heard of. Most people that take spore prints take them from isolated strains.  The first part I'm not entirely sure why, but id imagine it has to do more with constant breaking up and recovering using up energy than it does actually stretching the mycelium.


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Offlinetump
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Registered: 03/17/16
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Re: Why too many generations loses vitality/vigor? [Re: TravelAgency] * 1
    #23643785 - 09/14/16 04:14 AM (7 years, 4 months ago)

First one is dividing of cells two many times and aging of its enzymes. I've heard of the second one as well but your confusing wild traits for wanted traits. If your growing as a hobbies and for personal use only then most traits on the stain don't matter to you
If you run a small mushroom farm there are traits you want to have like high BE. Or speed or fruiting at a normal temp.oysters with no would suck for hobbiest because as you point out making your own spores to save for later . But the small farm needs that trait to not keep replaceing 500 dallor air filter ever month. Once you gone form spores its all new traits and sub stains . It work never perform the same as the main isolate.


But if you want to keep the same iso then do so on agar plates just make uncommon agar every few transfers. Myc enzymes bluid immune to certain food source after so many transfers and time.
This works for grains as well. If you switched grains every 3 trainfers you can do 21+ grain to grain trafers bef


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OfflineGr0wer
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Registered: 09/16/03
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Re: Why too many generations loses vitality/vigor? [Re: tump]
    #23644837 - 09/14/16 02:43 PM (7 years, 4 months ago)

I feel the most important part of not taking spawn too many generations is the chances of hidden contamination such as yeast or bacteria thats not super visible like green/black/red mold


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OfflineFerather
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Re: Why too many generations loses vitality/vigor? [Re: Gr0wer]
    #23645027 - 09/14/16 03:43 PM (7 years, 4 months ago)

I've recently learned that it can be very strain specific. A good example is Golden oyster.
Golden does not like being expanded too much, it loses genetics very fast.

However its brother variant Tarragon is the opposite.
It gets stronger as you expand G1-G2-G3.

Another example is Summer oyster which has lots of different variety's.
Which shows it adapts genetically, and changes character.

So the conclusion is strains can be genetically weak.
If you reduced genetics, spores will be weaker.


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OfflineJanuaryWolf
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Re: Why too many generations loses vitality/vigor? [Re: Ferather]
    #23645787 - 09/14/16 08:53 PM (7 years, 4 months ago)

Has anyone experienced an older culture having mutated fruit bodies?


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Offlinetump
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Re: Why too many generations loses vitality/vigor? [Re: JanuaryWolf]
    #23645880 - 09/14/16 09:30 PM (7 years, 4 months ago)

I have. And i also know that will still air box work or using any rubbing achol that hits the culture at any time causes mutated parts.


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OfflineFerather
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Re: Why too many generations loses vitality/vigor? [Re: tump]
    #23646608 - 09/15/16 05:19 AM (7 years, 4 months ago)

Bleach mutates fruit body's too!


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

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Invisibleflyontoast
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Registered: 08/20/16
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Re: Why too many generations loses vitality/vigor? [Re: Ferather]
    #23647599 - 09/15/16 01:57 PM (7 years, 4 months ago)

Awesome info everyone, thanks.

Quote:

Ferather said:
I've recently learned that it can be very strain specific. A good example is Golden oyster.
Golden does not like being expanded too much, it loses genetics very fast.

However its brother variant Tarragon is the opposite.
It gets stronger as you expand G1-G2-G3.





I've read (not experienced) what a strain looks like when it gets weaker. Might try pushing my strains past G5 soon. What does a strain getting "stronger" look like? Faster colonization, more rhizomatic-looking myc?


--------------------
:mushroom: My trade list :mushroom:
Looking for strong terrestrial fruiters for an outdoor beds experiment:
Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors.
Also, any commercially viable Pleurotus, cold or hot strains.
Thanks for the Q&A, trades, and all the posters & teachers that have come before us  :skol:


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OfflineFerather
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Re: Why too many generations loses vitality/vigor? [Re: flyontoast]
    #23648089 - 09/15/16 04:53 PM (7 years, 4 months ago)

Strong growth from a Golden clone G2:



Weak growth from a Golden clone G4:



--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineTravelAgency
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Re: Why too many generations loses vitality/vigor? [Re: Ferather]
    #23648139 - 09/15/16 05:13 PM (7 years, 4 months ago)

Nice, same momma culture?


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OfflineFerather
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Re: Why too many generations loses vitality/vigor? [Re: TravelAgency]
    #23649523 - 09/16/16 04:10 AM (7 years, 4 months ago)

Yes, from my strong outdoor grow:



I cloned a tuber.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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Offlinedrake89
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Re: Why too many generations loses vitality/vigor? [Re: Ferather]
    #23649768 - 09/16/16 07:40 AM (7 years, 4 months ago)

Molecular machinery degrades as mutations accumulate during accelerated cell division.  You're asking a lot of mycelium when you constantly transfer it.


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OfflineJanuaryWolf
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Registered: 01/04/15
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Re: Why too many generations loses vitality/vigor? [Re: drake89]
    #23654404 - 09/17/16 10:53 PM (7 years, 4 months ago)

So then would cloning from a fruitbody be the same as taking a tissue of mycelium since the organism is the same age and just as "mature"?


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OfflineTravelAgency
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Re: Why too many generations loses vitality/vigor? [Re: JanuaryWolf]
    #23654803 - 09/18/16 04:34 AM (7 years, 4 months ago)

Great question JW, I would believe it to be so off of an isolated strain, though less so than straight transfers, however for spores may be entirely the opposite- however I must denote this is entirely conjecture based off of my limited knowledge.


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OfflineFerather
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Re: Why too many generations loses vitality/vigor? [Re: TravelAgency]
    #23654839 - 09/18/16 05:29 AM (7 years, 4 months ago)

A young tissue sample that is still growing out, such as a new fruit, tuber, primordia.
Is more likely to respond and grow out immediately. Teenager vs old man.

Its possible for dormant genes to be passed onto spores.
However my knowledge of spores is also limited.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineFerather
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Re: Why too many generations loses vitality/vigor? [Re: Ferather]
    #23657798 - 09/19/16 05:23 AM (7 years, 4 months ago)

As if by destiny Golden oyster presents itself with the solution.

Here is a G5 clone to WL Tek paper block, and its fruits.

   

Here is the same G5, cased, put outside.

   

----

It took about 2 months, but now full power and colour.

Triggered by heavy rainfall and high humidity.
I used a wood rich compost casing.

King oyster does the same.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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OfflineFerather
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Re: Why too many generations loses vitality/vigor? [Re: Ferather]
    #23660751 - 09/20/16 05:41 AM (7 years, 4 months ago)

Here is next day on those Golden oyster G5 outdoor fruits:

   

18°C - 65% Rh, high substrate water content.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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Invisibleflyontoast
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Registered: 08/20/16
Posts: 258
Re: Why too many generations loses vitality/vigor? [Re: Ferather]
    #23662616 - 09/20/16 06:29 PM (7 years, 4 months ago)

Sorry, but what am I observing here? I'm not being snarky, I don't know what is "the solution" to which problem? lol
I like the look of those oysters coming up through the casing. I'm actually shopping around for King oysters now (might start a new thread about that soon, ie. knowing what you are buying and what to look for).


--------------------
:mushroom: My trade list :mushroom:
Looking for strong terrestrial fruiters for an outdoor beds experiment:
Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors.
Also, any commercially viable Pleurotus, cold or hot strains.
Thanks for the Q&A, trades, and all the posters & teachers that have come before us  :skol:


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OfflineFerather
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Registered: 03/19/15
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Re: Why too many generations loses vitality/vigor? [Re: flyontoast]
    #23664177 - 09/21/16 05:58 AM (7 years, 4 months ago)

Its to do with many things, and I would love explain all of them in detail.
I am at work at the moment, I will explain this evening.

Here they are day 3, now 22°C - 65% Rh.

   

Medium Rh, High water content.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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