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Offlinebr33zy
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*Answered* - Spore to Agar - Inoculation Loop vs Sterile Cotton Swab
    #23643068 - 09/13/16 09:24 PM (7 years, 5 months ago)

I have been getting into agar work recently. I use small jelly jars with RTV self healing injection ports when using a spore syringe to agar with much success, but I want to transfer spores from prints I made to agar to get some more plates going. I am trying to decide between purchasing inoculation loops or disposable sterile 6in. cotton swabs. I am leaning towards the swabs because they are cheap :money: and seem convenient. What do you guys prefer? Is there any drawback to using the swabs instead of inoculation loops? :takingnotes: Thanks for your input! :sunny:

Update:
Answered
Going with this method (https://www.shroomery.org/forums/showflat.php/Number/22556356).

Edited by br33zy (09/13/16 09:43 PM)

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OfflineX2QE
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Re: Spore to Agar - Inoculation Loop vs Sterile Cotton Swab [Re: br33zy]
    #23643115 - 09/13/16 09:35 PM (7 years, 5 months ago)

Maybe give making your own swabs a shot if you want to save some cash.
https://www.shroomery.org/forums/showflat.php/Number/22556356

I have not tried it myself but I don't see why it would not work.

EDIT: You could also just scrape some off with your scalpel.

Edited by X2QE (09/13/16 09:37 PM)

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Offlinethemadextractor
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Re: Spore to Agar - Inoculation Loop vs Sterile Cotton Swab [Re: br33zy]
    #23643123 - 09/13/16 09:37 PM (7 years, 5 months ago)

It'll be cheaper to use inoculation loops over time just due to the reusable nature. I made my own loop and like it. Some one on here recommended using a twist  tie with the plastic covering removed.  It works well and you can customize the loop size. I haven't had much luck with swabs but so haven't really used them for prints. So just my input.

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OfflineX2QE
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Re: Spore to Agar - Inoculation Loop vs Sterile Cotton Swab [Re: themadextractor]
    #23643128 - 09/13/16 09:40 PM (7 years, 5 months ago)

What did you make your loop out of? I read nichrome wire is usually used.

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Offlinebr33zy
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Re: Spore to Agar - Inoculation Loop vs Sterile Cotton Swab [Re: X2QE] * 1
    #23643131 - 09/13/16 09:40 PM (7 years, 5 months ago)

Nice, this is awesome and super simple. Thanks for the link man.

Quote:

X2QE said:
Maybe give making your own swabs a shot if you want to save some cash.
https://www.shroomery.org/forums/showflat.php/Number/22556356

I have not tried it myself but I don't see why it would not work.

EDIT: You could also just scrape some off with your scalpel.




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Offlinebr33zy
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Re: Spore to Agar - Inoculation Loop vs Sterile Cotton Swab [Re: themadextractor]
    #23643133 - 09/13/16 09:42 PM (7 years, 5 months ago)

Quote:

themadextractor said:
It'll be cheaper to use inoculation loops over time just due to the reusable nature. I made my own loop and like it. Some one on here recommended using a twist  tie with the plastic covering removed.  It works well and you can customize the loop size. I haven't had much luck with swabs but so haven't really used them for prints. So just my input.




Thanks for your input.


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Please sign the petition HERE to reverse the DEA ban on Kratom!!!!!


The fear of the LORD is the beginning of knowledge, but fools despise wisdom and instruction.
Proverbs 1:7

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OfflineX2QE
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Re: Spore to Agar - Inoculation Loop vs Sterile Cotton Swab [Re: br33zy]
    #23643159 - 09/13/16 09:48 PM (7 years, 5 months ago)

No problem. Thanks to eatyualive for the write up  :smile:

Good luck. Let us know how it goes. :super:

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Offlinebr33zy
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Re: Spore to Agar - Inoculation Loop vs Sterile Cotton Swab [Re: X2QE]
    #23643163 - 09/13/16 09:49 PM (7 years, 5 months ago)

Will do friend! :sunny:


--------------------
Please sign the petition HERE to reverse the DEA ban on Kratom!!!!!


The fear of the LORD is the beginning of knowledge, but fools despise wisdom and instruction.
Proverbs 1:7

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

:sporedrop:      :tardpig:      :sporedrop:

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Offlinethemadextractor
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Re: Spore to Agar - Inoculation Loop vs Sterile Cotton Swab [Re: br33zy]
    #23643321 - 09/13/16 11:00 PM (7 years, 5 months ago)

The twist tie from bread bags and shit. Then I put it in my exacto knife handle and screw it tight. I used a really long twist tie that came with a cord for my tv. It was coated in plastic so I used wire strippers. Paper ones can just be burned off.

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OfflineShineonyoucrazy
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Re: Spore to Agar - Inoculation Loop vs Sterile Cotton Swab [Re: themadextractor]
    #23678980 - 09/26/16 07:46 AM (7 years, 5 months ago)

You can get nicrome wire in a variety of guages from most e cig vapor shops.  A 25 to 50 foot spool will be give or take $10.


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InvisibleMad Season
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Re: Spore to Agar - Inoculation Loop vs Sterile Cotton Swab [Re: Shineonyoucrazy]
    #23679100 - 09/26/16 08:37 AM (7 years, 5 months ago)

You can go to any arts and crafts store and look for clay tools.

But yeah swabs are good too! :smile:


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OfflinePeteyboy
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Re: Spore to Agar - Inoculation Loop vs Sterile Cotton Swab [Re: Mad Season]
    #23679203 - 09/26/16 09:23 AM (7 years, 5 months ago)

I didn't know this was a tek but this is what I have been doing and I love it!


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