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InvisibleYarry
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Making a sterile syringe from wild
    #2364285 - 02/21/04 05:22 PM (12 years, 9 months ago)

I have a wild mushroom with no spores, and was wondering the following. Ive heard its possible to blend up sterile mushrooms in sterile water to make a syringe out of. I have a wild mushroom which id like to make a syringe out of, how can i best ensure sterility?


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Re: Making a sterile syringe from wild [Re: Yarry]
    #2364305 - 02/21/04 05:27 PM (12 years, 9 months ago)

Using sterile technique, you can remove some of the interior of the shroom and plate it out on agar, or use it to inoculate a dextrose solution. You could go the route of blending, but everything must be sterile. An Eberbach would be ideal for your purposes, but expensive.


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OfflineGr0wer
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Re: Making a sterile syringe from wild [Re: Yarry]
    #2365300 - 02/21/04 09:58 PM (12 years, 9 months ago)

You can skin the shrooms stem, put it in a 1 pint jar with cooled boiled water and some broken glass. Shake this to break up the stem and then poke a hole in the top and make a syringe. I think some hydrogen peroxide will help make it sterile too but i havent tried this.


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OfflineBaby_Hitler
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Re: Making a sterile syringe from wild [Re: Yarry]
    #2365306 - 02/21/04 10:00 PM (12 years, 9 months ago)

http://www.fungifun.org/cloning/

Or use peroxidated liquid culture, just use any liquid culture recipe and add about 3cc per pint of 3% Hydrogen Peroxide solution. The trick is to rip the mushroom open, don't cut it open. Cutting it will contaminate the inside surface with contaminants from the outside of the mushroom.


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OfflineScourge
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Re: Making a sterile syringe from wild [Re: Theo Nanacatl]
    #2372678 - 02/23/04 05:38 PM (12 years, 9 months ago)

I would go with Theo's recomendation.


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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