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OfflineShroomieNewbie
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White sediment in Malt Extract after PC
    #23642783 - 09/13/16 07:41 PM (7 years, 4 months ago)

Hey guys. I just finished sterilizing my first batch of Light Malt Extract in the PC for 20 mins at 15 psi. There appears to be some white sediment floating around in it. I have a piece of glass in the jar to break up the mycelium later on. Even swishing the contents around inside, it appears the sediment is there to stay. Is this normal/ok? Or is it going to hurt my LC?

I'll add that I used a half of a tablespoon of LME to 1/2 cup water.

Thanks!!



Edited by ShroomieNewbie (09/13/16 07:49 PM)


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23643164 - 09/13/16 09:50 PM (7 years, 4 months ago)

Replying so i can read later as im getting into agar too


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Re: White sediment in Malt Extract after PC [Re: spore-ty]
    #23643208 - 09/13/16 10:03 PM (7 years, 4 months ago)

I think this is a liquid culture.

:shrug: About the sediment OP.


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OfflineTheLongComma
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Re: White sediment in Malt Extract after PC [Re: X2QE]
    #23646489 - 09/15/16 03:24 AM (7 years, 4 months ago)

Un-dissolved powder, I'm guessing.

Try boiling longer when mixing pre-PC so that everything dissolves better.


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Re: White sediment in Malt Extract after PC [Re: TheLongComma]
    #23651362 - 09/16/16 07:37 PM (7 years, 4 months ago)

It didn't look like this before being PC'd.

I just knocked it up with 3 cc's of GLC about 24 hrs ago. I'll update whatever happens.


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23651370 - 09/16/16 07:41 PM (7 years, 4 months ago)

It looks overcooked. Which is odd for 20 mins at 15 psi. I'd drop it down to 15 mins. Also did you vent too much? A huge pressure drop also overcooks it.


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Re: White sediment in Malt Extract after PC [Re: Mad Season]
    #23651388 - 09/16/16 07:47 PM (7 years, 4 months ago)

Seeing as I only used LME I'm with you on the fact that it'd be odd that it's overcooked. But no, I let the pressure drop naturally. I made sure the PC was room temp to the touch before I did anything, just like for anything else I PC.

Edit: I only let it vent for about 5 minutes before throwing the rocker on.


Edited by ShroomieNewbie (09/16/16 07:48 PM)


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23651412 - 09/16/16 07:56 PM (7 years, 4 months ago)

How much did you put in? 2-4%?


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Re: White sediment in Malt Extract after PC [Re: Mad Season]
    #23651520 - 09/16/16 08:48 PM (7 years, 4 months ago)

https://www.shroomery.org/9145/Liquid-Culture-Basics

Following the above link to LC basics, my dilution is at just about 4% exactly.


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23651626 - 09/16/16 09:37 PM (7 years, 4 months ago)

sediment is pretty normal for malt extract. hence why I boil it first and then decant or filter then pressure cook. besides the boil step gets the agar in.


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Re: White sediment in Malt Extract after PC [Re: bodhisatta]
    #23651868 - 09/16/16 11:57 PM (7 years, 4 months ago)

See, following the above link I didn't boil. Just super hot water and mixed in. I will go ahead and make another LC culture just for shits n giggles, but I will boil and filter before I PC. That sounds pretty fool proof.

I'm going to just leave this one that's done and see what it does. The sediment shouldn't hurt anything in rye jars right? Does it in any way promote contamination more than average?

Thanks for all the replies.


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23676872 - 09/25/16 02:00 PM (7 years, 4 months ago)

I just wanted to leave an update here. I'm day 9 into the above LC and it's looking beautiful. About 3/4 colonized from the GLC that was used to inoculate. I wasnt sure what to expect since it's my first but it's superr clean with zero contams. I swish it around a couple times a day to redistribute the myc for more coverage.

I will be inoculating some rye berries hopefully in a few days or less.


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23676888 - 09/25/16 02:05 PM (7 years, 4 months ago)

You don't know it has 0 contamination til you test it.....

If I had to take a wild guess I would say it probably is contaminated. Using it on spawn before testing in this case is dumb :shrug:


Edited by bodhisatta (09/25/16 02:07 PM)


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OfflineShroomieNewbie
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Re: White sediment in Malt Extract after PC [Re: bodhisatta]
    #23676909 - 09/25/16 02:12 PM (7 years, 4 months ago)

How can such an assumption of contam be made? Just curious. I literally have nothing to lose to inoc some rye jars.


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23676922 - 09/25/16 02:16 PM (7 years, 4 months ago)

Well you inoculated a LC with a GLC so that's just fucking asking for it.

And the no boil shit. That's basically 99% chance of fucking you.


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Re: White sediment in Malt Extract after PC [Re: bodhisatta]
    #23676933 - 09/25/16 02:22 PM (7 years, 4 months ago)

What would you recommend for inoculating LC with? Multi spores?

And it just blows that the sticked liquid culture basics here on the shroomery gives me false info. Why is it even there...


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23676959 - 09/25/16 02:33 PM (7 years, 4 months ago)

LC should only be inoculated with agar

Stickied? Which one?

Inoculation of a LC with MS is the worst way

At the end of the day you have to vet info yourself.


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Re: White sediment in Malt Extract after PC [Re: bodhisatta]
    #23676978 - 09/25/16 02:43 PM (7 years, 4 months ago)

I linked the LC Basics above your first post. I guess it's not stickied, but instead looks integrated into the site.

At which point can I use the LC to try a test jar? I'm getting ancy now. I'd say theres quite a bit larger than a golfball size of cloud inside the jar.

Thanks.


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23676985 - 09/25/16 02:45 PM (7 years, 4 months ago)

7-10 days then test it out


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Offlineenlightenment
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Re: White sediment in Malt Extract after PC [Re: bodhisatta]
    #23676990 - 09/25/16 02:48 PM (7 years, 4 months ago)

Quote:

bodhisatta said:
7-10 days then test it out



The jar looks pretty small. If there is already a mycelium 'cloud' the size of a golfball I would make a syringe right now. But that's just my opinion...


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Re: White sediment in Malt Extract after PC [Re: enlightenment]
    #23676998 - 09/25/16 02:50 PM (7 years, 4 months ago)

Alright yeah. I'm entering day 9 here in a few hours and it is small for that point alone. I'm going to sterilize a rye jar right now and inoculate it tomorrow.



Edited by ShroomieNewbie (09/25/16 02:52 PM)


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23677009 - 09/25/16 02:55 PM (7 years, 4 months ago)

:billymaythumbup: keep us posted. You could open a new grow log thread after inoculation.  :yesnod:


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Re: White sediment in Malt Extract after PC [Re: enlightenment]
    #23677132 - 09/25/16 03:40 PM (7 years, 4 months ago)

I definitely will!

Let's see if this engineering techician can grow some mushrooms. Haha

:evil:


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OfflineShroomieNewbie
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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23701055 - 10/02/16 05:35 PM (7 years, 3 months ago)

Just for the haters who don't believe a simple and sterile GLC to LC can produce a wonderful LC syringe .. or 6. Point being, don't be a dick to someone just because they have a low post count. Everyone is human.

Enjoy



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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23701061 - 10/02/16 05:36 PM (7 years, 3 months ago)

Good job. :super:
The post count doesn't matter... We are all constantly learning.. I have been growing mushrooms for more than 10 years and i am still learning because time and research offer a wide range of new possibilities. Keep up your work


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InvisibleMad Season
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Re: White sediment in Malt Extract after PC [Re: enlightenment]
    #23701070 - 10/02/16 05:41 PM (7 years, 3 months ago)

So no fruits at all, hell not even a fully colonized jar?

looks clean at this stage..


Beginning to have some look bacterial, but had to wait til most of it was colonized..



Lots of bacteria...


Almost all jars look clean at the beginning..


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Re: White sediment in Malt Extract after PC [Re: Mad Season]
    #23701076 - 10/02/16 05:44 PM (7 years, 3 months ago)

Of course you never know if everything is alright until full colonization... But if the syringe would be contaminated as hell you would see it right now. Chances are high that the jars will colonize just fine.. lets wait. I am sure he keeps us posted...


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InvisibleMad Season
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Re: White sediment in Malt Extract after PC [Re: enlightenment]
    #23701085 - 10/02/16 05:48 PM (7 years, 3 months ago)



Here's what a jar covered top to bottom in bacteria looks like. Bacteria is almost impossible to see without mycelium showing its reaction to it. As seen here:



Just saying that there's no way you can say it's clean with barely any myc there. Fully colonized and it's a different story.


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OfflineGreg
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Re: White sediment in Malt Extract after PC [Re: enlightenment]
    #23701089 - 10/02/16 05:50 PM (7 years, 3 months ago)

You can't see bacteria in LC.
Here is some extremely bacterial winecap LC.
Can you see the bacteria?


This particular culture took me 4 transfers to clean.
It colonizes a jar oh probably halfway (looks completely normal too), before it smells of the black plague and sadness.


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Re: White sediment in Malt Extract after PC [Re: Greg]
    #23701094 - 10/02/16 05:51 PM (7 years, 3 months ago)

:lol: for veterinary use only dude!! XD


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Re: White sediment in Malt Extract after PC [Re: Mad Season]
    #23701105 - 10/02/16 05:55 PM (7 years, 3 months ago)

That's how I grow mushrooms, live animal substrate. :lol:


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Re: White sediment in Malt Extract after PC [Re: Mad Season]
    #23701623 - 10/02/16 08:57 PM (7 years, 3 months ago)

Quote:

Mad Season said:
So no fruits at all, hell not even a fully colonized jar?

looks clean at this stage..


Beginning to have some look bacterial, but had to wait til most of it was colonized..



Lots of bacteria...


Almost all jars look clean at the beginning..




All I have to do is zoom in on the 'looks clean at this stage" and I see more than 3 jars with caramel in it. Lol Sorry for being critical on how "clean" they look.


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23701646 - 10/02/16 09:02 PM (7 years, 3 months ago)

And yes, I will keep this thread updated until the jar is 100% colonized. I live in a very clean town house with zero mold, barely any dust, and I use a SAB with all precautionary measures. Contams don't just form, they enter your "play" area.

Let's see how clean my play area really is.:mushroom2: :sporedrop:  :sporedrop:


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InvisibleMad Season
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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23701794 - 10/02/16 09:53 PM (7 years, 3 months ago)

Quote:

ShroomieNewbie said:
Quote:

Mad Season said:
So no fruits at all, hell not even a fully colonized jar?

looks clean at this stage..


Beginning to have some look bacterial, but had to wait til most of it was colonized..



Lots of bacteria...


Almost all jars look clean at the beginning..




All I have to do is zoom in on the 'looks clean at this stage" and I see more than 3 jars with caramel in it. Lol Sorry for being critical on how "clean" they look.



You mean agar wedges?... Yeah that's common with agar 2 grain :facepalm:. I seriously hope you don't mean the agar wedges lol

Quote:

ShroomieNewbie said:
And yes, I will keep this thread updated until the jar is 100% colonized. I live in a very clean town house with zero mold, barely any dust, and I use a SAB with all precautionary measures. Contams don't just form, they enter your "play" area.

Let's see how clean my play area really is.:mushroom2: :sporedrop:  :sporedrop:



Research on endospores. Farmers spray grains with them as a form of natural pesticides. This is the reason why you have to PC grains much longer than you normally would for sterilization, and can easily survive a 3 hour pc cycle. Aloha does a double cycle for 4 hours each to kill endospores (sterilization of most media is less than 20 minutes @ 15 psi). That's why grain LCs are considered bad practice. Can they work? Sure. But you're literally just gambling. It's the same as tossing a coin.


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (10/02/16 10:13 PM)


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Re: White sediment in Malt Extract after PC [Re: Mad Season]
    #23701840 - 10/02/16 10:13 PM (7 years, 3 months ago)

Endospores are easily killed by a PC however protected by grains it becomes difficult also sterilization time depends on initial CFU load.

:whathesaid:


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