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OfflineShroomieNewbie
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White sediment in Malt Extract after PC
    #23642783 - 09/13/16 07:41 PM (7 years, 4 months ago)

Hey guys. I just finished sterilizing my first batch of Light Malt Extract in the PC for 20 mins at 15 psi. There appears to be some white sediment floating around in it. I have a piece of glass in the jar to break up the mycelium later on. Even swishing the contents around inside, it appears the sediment is there to stay. Is this normal/ok? Or is it going to hurt my LC?

I'll add that I used a half of a tablespoon of LME to 1/2 cup water.

Thanks!!



Edited by ShroomieNewbie (09/13/16 07:49 PM)


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Offlinespore-ty
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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23643164 - 09/13/16 09:50 PM (7 years, 4 months ago)

Replying so i can read later as im getting into agar too


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Re: White sediment in Malt Extract after PC [Re: spore-ty]
    #23643208 - 09/13/16 10:03 PM (7 years, 4 months ago)

I think this is a liquid culture.

:shrug: About the sediment OP.


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OfflineTheLongComma
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Re: White sediment in Malt Extract after PC [Re: X2QE]
    #23646489 - 09/15/16 03:24 AM (7 years, 4 months ago)

Un-dissolved powder, I'm guessing.

Try boiling longer when mixing pre-PC so that everything dissolves better.


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OfflineShroomieNewbie
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Re: White sediment in Malt Extract after PC [Re: TheLongComma]
    #23651362 - 09/16/16 07:37 PM (7 years, 4 months ago)

It didn't look like this before being PC'd.

I just knocked it up with 3 cc's of GLC about 24 hrs ago. I'll update whatever happens.


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InvisibleMad Season
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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23651370 - 09/16/16 07:41 PM (7 years, 4 months ago)

It looks overcooked. Which is odd for 20 mins at 15 psi. I'd drop it down to 15 mins. Also did you vent too much? A huge pressure drop also overcooks it.


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OfflineShroomieNewbie
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Re: White sediment in Malt Extract after PC [Re: Mad Season]
    #23651388 - 09/16/16 07:47 PM (7 years, 4 months ago)

Seeing as I only used LME I'm with you on the fact that it'd be odd that it's overcooked. But no, I let the pressure drop naturally. I made sure the PC was room temp to the touch before I did anything, just like for anything else I PC.

Edit: I only let it vent for about 5 minutes before throwing the rocker on.


Edited by ShroomieNewbie (09/16/16 07:48 PM)


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InvisibleMad Season
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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23651412 - 09/16/16 07:56 PM (7 years, 4 months ago)

How much did you put in? 2-4%?


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Re: White sediment in Malt Extract after PC [Re: Mad Season]
    #23651520 - 09/16/16 08:48 PM (7 years, 4 months ago)

https://www.shroomery.org/9145/Liquid-Culture-Basics

Following the above link to LC basics, my dilution is at just about 4% exactly.


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23651626 - 09/16/16 09:37 PM (7 years, 4 months ago)

sediment is pretty normal for malt extract. hence why I boil it first and then decant or filter then pressure cook. besides the boil step gets the agar in.


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Re: White sediment in Malt Extract after PC [Re: bodhisatta]
    #23651868 - 09/16/16 11:57 PM (7 years, 4 months ago)

See, following the above link I didn't boil. Just super hot water and mixed in. I will go ahead and make another LC culture just for shits n giggles, but I will boil and filter before I PC. That sounds pretty fool proof.

I'm going to just leave this one that's done and see what it does. The sediment shouldn't hurt anything in rye jars right? Does it in any way promote contamination more than average?

Thanks for all the replies.


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23676872 - 09/25/16 02:00 PM (7 years, 4 months ago)

I just wanted to leave an update here. I'm day 9 into the above LC and it's looking beautiful. About 3/4 colonized from the GLC that was used to inoculate. I wasnt sure what to expect since it's my first but it's superr clean with zero contams. I swish it around a couple times a day to redistribute the myc for more coverage.

I will be inoculating some rye berries hopefully in a few days or less.


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InvisiblebodhisattaMDiscordReddit
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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23676888 - 09/25/16 02:05 PM (7 years, 4 months ago)

You don't know it has 0 contamination til you test it.....

If I had to take a wild guess I would say it probably is contaminated. Using it on spawn before testing in this case is dumb :shrug:


Edited by bodhisatta (09/25/16 02:07 PM)


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OfflineShroomieNewbie
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Re: White sediment in Malt Extract after PC [Re: bodhisatta]
    #23676909 - 09/25/16 02:12 PM (7 years, 4 months ago)

How can such an assumption of contam be made? Just curious. I literally have nothing to lose to inoc some rye jars.


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23676922 - 09/25/16 02:16 PM (7 years, 4 months ago)

Well you inoculated a LC with a GLC so that's just fucking asking for it.

And the no boil shit. That's basically 99% chance of fucking you.


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Re: White sediment in Malt Extract after PC [Re: bodhisatta]
    #23676933 - 09/25/16 02:22 PM (7 years, 4 months ago)

What would you recommend for inoculating LC with? Multi spores?

And it just blows that the sticked liquid culture basics here on the shroomery gives me false info. Why is it even there...


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23676959 - 09/25/16 02:33 PM (7 years, 4 months ago)

LC should only be inoculated with agar

Stickied? Which one?

Inoculation of a LC with MS is the worst way

At the end of the day you have to vet info yourself.


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Re: White sediment in Malt Extract after PC [Re: bodhisatta]
    #23676978 - 09/25/16 02:43 PM (7 years, 4 months ago)

I linked the LC Basics above your first post. I guess it's not stickied, but instead looks integrated into the site.

At which point can I use the LC to try a test jar? I'm getting ancy now. I'd say theres quite a bit larger than a golfball size of cloud inside the jar.

Thanks.


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Re: White sediment in Malt Extract after PC [Re: ShroomieNewbie]
    #23676985 - 09/25/16 02:45 PM (7 years, 4 months ago)

7-10 days then test it out


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Offlineenlightenment
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Re: White sediment in Malt Extract after PC [Re: bodhisatta]
    #23676990 - 09/25/16 02:48 PM (7 years, 4 months ago)

Quote:

bodhisatta said:
7-10 days then test it out



The jar looks pretty small. If there is already a mycelium 'cloud' the size of a golfball I would make a syringe right now. But that's just my opinion...


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