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OfflineParallelTimeStamp
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Spore re-hydration
    #23642399 - 09/13/16 05:48 PM (7 years, 4 months ago)

I just recently found some spores of Panaeolus cyanescens from a stash I had from 7+ years ago. They have been in a zip lock bag in the dark in a box the whole time, never seeing light until the other day when I remembered about them pulled them out. I put them in some R O water that I sterilized and had in the fridge. 30hrs later I put them in a syringe and looked at the spores under my microscope. They looked good and as they have re hydrated with the exception of a few bent looking ones.

I'm not sure they will do anything but I have heard spores could go to space and back and survive the vacuum so I proceeded to make a mix of horse poo, straw, Gypsum, and some verm and pasteurized them for 2 hrs.
I know these cyanescens like wood and dont grow on poo but i didn't really think after 7+ yrs of being put away id get anything but contamination in my jars so I used this medium just for shits and giggles.
Two days into inoculation of jars and I think im already seeing mycelium growth, but its to early to say for sure, but it sure does appear to be doing something. Ill take pictures here in a couple days and show the progress of my jars once the growth can be seen.

My question about this is what is your opinion? Do you think the spores re-hydrated after 7+ yrs? Do you think these jars will work or just come out with contamination or just do nothing at all?

Thank you for your feed back and look forward to an convo about the situation.


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"Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"


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OfflinecronicrFacebook
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Re: Spore re-hydration [Re: ParallelTimeStamp]
    #23642482 - 09/13/16 06:14 PM (7 years, 4 months ago)

you are likley seeing mold lol...we use grains for bulk and the cyans you have are dung lovers


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OfflineParallelTimeStamp
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Re: Spore re-hydration [Re: cronicr]
    #23642511 - 09/13/16 06:21 PM (7 years, 4 months ago)

They are dung loving, thank you for that clear up, I guess then i did use the dung for a reason.....I was looking into wood loving mushrooms today and I think thats why I thought the ones I had were them...Sorry my mistake....anyway the ones i have are the  Panaeolus cyanescens.
I use rye seed for bulk myself, but again my spores are old as hell so I just wanted to start a jar culture with poo just to see if by some slim chance it works ill use that jar to make as spawn for a bulk.

I think your right though, it might just be mold hahaha these spores are old as hell and I heard they dont last like that specially dry but then again what i heard could be wrong. Thats why I opened this thread. Thank you for your reply by the way your much appreciated.


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"Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"


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OfflinecronicrFacebook
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Re: Spore re-hydration [Re: ParallelTimeStamp]
    #23642514 - 09/13/16 06:22 PM (7 years, 4 months ago)

got any pics


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OfflineParallelTimeStamp
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Re: Spore re-hydration [Re: cronicr]
    #23642526 - 09/13/16 06:25 PM (7 years, 4 months ago)

Ill take one for you right now but there 2 days old so might not see anything, ill go macro with it and see if that does anything....hold on ill get one for you


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"Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"


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OfflineParallelTimeStamp
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Re: Spore re-hydration [Re: ParallelTimeStamp]
    #23642553 - 09/13/16 06:35 PM (7 years, 4 months ago)

Dude, ok so i know now why i got confused and I must correct myself....I didnt use horse poo for these, i used the horse poo for a my chitwans Ive got a nice poo brink i inoculated and i used jars to pasteurize it and thats why i thought I used poo in these other jars .....Ive got a lot of subs inoculated and jars going so im starting to loose track and probably need to slow down........lmao...naaa cant go slow now the mycelium is moving fast lol. Anyway I used straw verm and brown rice for these jars cause i didnt wanna waste the horse poo just incase they go bad lol....those pics are coming right up, just had a couple but were blurry gonna make fix....pics in 5 min


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"Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"


Edited by ParallelTimeStamp (09/13/16 06:48 PM)


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OfflineParallelTimeStamp
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Re: Spore re-hydration [Re: ParallelTimeStamp]
    #23642589 - 09/13/16 06:44 PM (7 years, 4 months ago)

Ok, You cant see much as ive mentioned because there 2 days old. Im really hoping i can bring them back from the dead but i have my doubts lol...I will keep these jars and pics coming until I see nasty and I toss them.



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"Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"


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Invisibleh0ldthedoor
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Re: Spore re-hydration [Re: ParallelTimeStamp]
    #23642609 - 09/13/16 06:49 PM (7 years, 4 months ago)

Very clear photo, if only my agar plates would come out that clear..
:tellmeastory:


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Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

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OfflineParallelTimeStamp
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Re: Spore re-hydration [Re: h0ldthedoor]
    #23642619 - 09/13/16 06:51 PM (7 years, 4 months ago)

Thank you I was actually laying on the floor to take that pic lol


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"Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"


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OfflineParallelTimeStamp
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Re: Spore re-hydration [Re: ParallelTimeStamp]
    #23649933 - 09/16/16 09:07 AM (7 years, 4 months ago)

ok, so heres an update on those jars with the 7+ yr old spores i tried to bring back from the dead, there now five days old and.........drumb roll please........and.....nothing haha.

I think its pretty safe to say no matter how you keep your spores once dried that much there history. I will go as far as saying when you happen to have some spores in a bag from some dried mushrooms they will not germinate after that long even if they were in the dark the whole time as mine were. When I first got them I was able to re hydrate them easy and culture jars no problem, but that was when the spores were probably only weeks old at most. Now 7+yrs later not so young lol, and very dry.

on the plus side I got an awesome slide of spores for my microscope ill be holding onto for future reference so at least I won there, but technically didnt loose at all cause it was science and every out come in science is a result no matter if its the one you wanted or not.....I'd call this one a success even though the jars didn't culture, because my plan was to set out and learn something and I did. Hope I was able to help others along the way as well.

P.S.
I'd take a pic but they look the same as the one I took above lol, and will be dumping them out so I can keep the jars and reuse them, I will keep one of them for shits in giggles for another week and update you guys again, just in case something crazy happens that's not contaminates, but will have a pic of the saved jar regardless of its outcome before its dumped.


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"Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"


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InvisibleMad Season
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Re: Spore re-hydration [Re: ParallelTimeStamp]
    #23650286 - 09/16/16 11:44 AM (7 years, 4 months ago)

I germinated a 4 year old print very easily. It was very dry, and only 3 years younger. 5 days is literally nothing in terms of germination. There's also many options to do if they're stubborn. I wouldn't count it out yet.

Examples of germinating stubborn spores.. forced hydration, agar sandwich/hot pour, charcoal agar, nutritious and wet agar, snake venom, etc.


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OfflineParallelTimeStamp
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Re: Spore re-hydration [Re: Mad Season]
    #23652539 - 09/17/16 09:18 AM (7 years, 4 months ago)

Quote:

Mad Season said:
I germinated a 4 year old print very easily. It was very dry, and only 3 years younger. 5 days is literally nothing in terms of germination. There's also many options to do if they're stubborn. I wouldn't count it out yet.

Examples of germinating stubborn spores.. forced hydration, agar sandwich/hot pour, charcoal agar, nutritious and wet agar, snake venom, etc.





My particular spores were from the bottom of a bag I bought and I salvaged them and used them, once i had a couple jars I took the rest (still loose in the bag) and hid them in the dark for storage. I didnt mean to leave them that long, but I totally forgot about them lol. Now over 7yrs later I tried for the hell of it to do something with them.

The one jar I kept has something going on with it, but i doubt its mycelium, but instead mold.....maybe. I'm keeping it just outta spite lol, but I believe because of the way they were kept and the handling they were goners to start, but we will see in a couple more days.

I believe if I had them as a spore print as you mentioned above they would have had a way better chance, regardless of that, I really appreciate your response and your info, I now have faith in the 4yr mark for a spore print and that is comforting because that means I don't necessarily need a LC to keep a stock. I will also look into the other stuff you mentioned above thank you.

P.s. Your pics of the dish art you have there are awesome, scary, but awesome lol.


--------------------
"Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"


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