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ParallelTimeStamp
Time Travler



Registered: 09/13/16
Posts: 38
Last seen: 4 years, 10 months
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Whats in your LC? 1
#23642283 - 09/13/16 05:08 PM (7 years, 4 months ago) |
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Hows it going guys? I wanted to have a friendly convo about LC's and whats in yours? I have 2 LC's at the moment myself, the one in the 600ml jar is cubensis Burma and in the 300ml jar I have cubensis Nepal Chitwans. My LC is made from R O water and Karo light syrup. My Chitwan LC has been going for only 3 days and is looking good, my Burma LC has been going for just over a week now and is filling with growth.
I use one tbsp of karo per 300ml of R O water, I then steam sterilize for 2-3 hrs and it works fine for me. My LC stays clear and the mycelium culture grows great and fast. I only ever use R O water for boiling, steaming, prep and cleaning. I do not and will not use tap water.
I was wondering whats in your LC? Both solution and mycelium culture?
-------------------- "Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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there's no essential nutrients and minerals, you would be better off using tap water. sugar LC is the worst. leftover grain soak water or malt extract work much better for LC than just Karo. YPD broth also works it's just YPD agar without the agar.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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i use the same shit that is in my agar...minus the agar
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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The fuck is this guys beef with tap water..?
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: Whats in your LC? [Re: Inocuole]
#23642401 - 09/13/16 05:49 PM (7 years, 4 months ago) |
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Quote:
Inocuole said: The fuck is this guys beef with tap water..?
Lmao
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ParallelTimeStamp
Time Travler



Registered: 09/13/16
Posts: 38
Last seen: 4 years, 10 months
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Re: Whats in your LC? [Re: Inocuole]
#23642428 - 09/13/16 05:59 PM (7 years, 4 months ago) |
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Lmao, Inocuole I have no beef with tap water, just choose not to use it do to the Chloride and Chlorine that they put in it around here. I have done fish tanks both fresh and salt for the past 16yrs of my life so R O water is just something i use cause i know its clear of the impurities i dont want to deal with. Sorry to make you think any other way.
Bodhisatta Thank you for that reply, this is why I started this thread. I am open to learning and I dont claim to know everything not even close lol. I like to hear other ideas and opinions. I will save my water from my rye seeds from now on and use that. Thank you again for that idea
-------------------- "Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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You need FAN free amino nitrogen. Plain sugar water LC has bad performance because of this and other nutrient deficiency
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ParallelTimeStamp
Time Travler



Registered: 09/13/16
Posts: 38
Last seen: 4 years, 10 months
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Bodhisatta, Thank you for that bit of information its actually the exact info Ive been looking for! I'm going to get some tomorrow. I dont want my LC's going bad.
If I make up some new jars and I take a syringe of my current culture and add it to my new solution jar will the little bit of sugars that are going to be with it hurt the new solution, or will it not matter because the culture will eat it up anyway? I believe it would be ok seeing it would be just food, but id like to know what you think.
-------------------- "Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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br33zy
Astro-Feline



Registered: 08/25/16
Posts: 97
Loc: Cat's Eye Nebula
Last seen: 7 years, 3 months
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Learned something new today. Thanks TC! 
Quote:
Trusted Cultivator said: You need FAN free amino nitrogen. Plain sugar water LC has bad performance because of this and other nutrient deficiency
-------------------- Please sign the petition HERE to reverse the DEA ban on Kratom!!!!! The fear of the LORD is the beginning of knowledge, but fools despise wisdom and instruction. Proverbs 1:7 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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ParallelTimeStamp
Time Travler



Registered: 09/13/16
Posts: 38
Last seen: 4 years, 10 months
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Re: Whats in your LC? [Re: br33zy]
#23643193 - 09/13/16 09:58 PM (7 years, 4 months ago) |
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I did to, and yes a very big thank you for that bit of knowledge, and I'm stoked that I could make a thread that helped myself and others learn today.....Sweet Score!!
-------------------- "Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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22 karo plus oats water lc jars. 13 apple juice lc jars. And 2 corn starch lc jars. If you want to think outside the box corn starch lc is the bomb
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RealmLAir
Stranger
Registered: 07/17/16
Posts: 94
Last seen: 2 years, 8 months
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Re: Whats in your LC? [Re: tump]
#23643452 - 09/13/16 11:43 PM (7 years, 4 months ago) |
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Quote:
tump said: 22 karo plus oats water lc jars. 13 apple juice lc jars. And 2 corn starch lc jars. If you want to think outside the box corn starch lc is the bomb
Pics Man please..
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ParallelTimeStamp
Time Travler



Registered: 09/13/16
Posts: 38
Last seen: 4 years, 10 months
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Re: Whats in your LC? [Re: RealmLAir]
#23643988 - 09/14/16 07:34 AM (7 years, 4 months ago) |
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yes some pics would be awesome please share....And thank you tump for your reply, ill look into those ingredients and look forward to seeing some pics of your LC's, if you would be so kind.
I'm after the ultimate solution for the longest life possible. I'm open to all ideas, but I will be buying malt thats a (FAN free amino nitrogen) today as Bodhisatta mentioned. Hes got a strong point. It makes sense that they would want an amino nitrogen, seeing cubensis love nitrogen in general. Hints one the reasons they love h-poo and c-poo.
-------------------- "Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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All fungi need more than sugar and water anyway.
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ParallelTimeStamp
Time Travler



Registered: 09/13/16
Posts: 38
Last seen: 4 years, 10 months
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I agree,
one must treat there fungi with respect for the fungi to respect them back and grow in the way they want them to. Proper care and proper food is the best sign of respect you could give.
-------------------- "Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Disagree. Straight and sugar will work.  Top picture is corn starch after 3 months. Bottom is koro plus oats water after 5 days. The only reason i dont use straight karo is to see when all food is eaten.
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LeeHarvOz
Homie



Registered: 10/13/10
Posts: 2,339
Last seen: 1 year, 7 days
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Quote:
Trusted Cultivator said: there's no essential nutrients and minerals, you would be better off using tap water. sugar LC is the worst. leftover grain soak water or malt extract work much better for LC than just Karo. YPD broth also works it's just YPD agar without the agar.
can i just take the water my rye has been soaking in to rehydrate it and sterilize it in jars with the appropriate lids to be used as LC? does anything else need to be added?
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ParallelTimeStamp
Time Travler



Registered: 09/13/16
Posts: 38
Last seen: 4 years, 10 months
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Re: Whats in your LC? [Re: tump]
#23644161 - 09/14/16 09:23 AM (7 years, 4 months ago) |
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very nice sir! Thank you for your pics. My karo culture is going strong at the moment itself, but Im totally with you in being able to see when the food is gone. Im trying to keep my culture alive for long time not starve it.
imo, I don't think Bodhisatta is disagreeing with sugar water in general cause we def know it works, do to so many using that method, but hes right when it comes to keeping it healthy.
Look at it as a cannabis plant (using cannabis cause its a good example, or fish tank plants would work too i suppose), but when growing a plant anyone can put a light bulb over it and water it and watch it grow, this is called "existing" the plant will not yield your desired look or status. When you give the proper amount of food, ph, and light then your plant does whats called "thriving" you will in this case achieve your desired look and or yield.
tump, im deff not saying that your not keeping your jars healthy or feeding them proper cause they look great and i will personally start adding something to help me see when the food is gone, thats a great idea and greatly appreciate that thought, you and Bodhisatta both have saved me many hrs of questioning if my culture was to just crap out, and for that I greatly appreciate you both.
LeeHarvOz I was wondering the same thing, and imo I think that if the rye seed itself is a great substrate and it lives great on the rye alone, id think that just rye water alone would work.
-------------------- "Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Whats in your LC? [Re: tump]
#23644175 - 09/14/16 09:29 AM (7 years, 4 months ago) |
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Quote:
tump said: Disagree. Straight and sugar will work.  Top picture is corn starch after 3 months. Bottom is koro plus oats water after 5 days. The only reason i dont use straight karo is to see when all food is eaten.
The fungi is elongating off its stored enery after a short period in search of more food.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Whats in your LC? [Re: LeeHarvOz]
#23644178 - 09/14/16 09:31 AM (7 years, 4 months ago) |
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Quote:
LeeHarvOz said:
Quote:
Trusted Cultivator said: there's no essential nutrients and minerals, you would be better off using tap water. sugar LC is the worst. leftover grain soak water or malt extract work much better for LC than just Karo. YPD broth also works it's just YPD agar without the agar.
can i just take the water my rye has been soaking in to rehydrate it and sterilize it in jars with the appropriate lids to be used as LC? does anything else need to be added?
Water it down 1:2 - 1:4. Let it settle in a jar for a few hours. Decant it off the sediment if you want it to be more clear.
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ParallelTimeStamp
Time Travler



Registered: 09/13/16
Posts: 38
Last seen: 4 years, 10 months
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Quote:
Trusted Cultivator said: Water it down 1:2 - 1:4. Let it settle in a jar for a few hours. Decant it off the sediment if you want it to be more clear.
Will the sediment provide any extra food or a place for the culture to take hold, or is it useless in that sense and just waste that makes your culture non clear and be more pron to contaminates?
-------------------- "Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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The sugar water is makes it prone to contaminants.. the sediment doesn't make it more prone to anything other than clumping.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Whats in your LC? [Re: Inocuole]
#23645364 - 09/14/16 05:50 PM (7 years, 4 months ago) |
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Sediment imo isn't anything worth keeping. If it's not dissolved its just something that's hard to sterilize.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Eh, I guess if there's a lot. I've only ever had a little bit of sediment with anything. It's definitely a pain in the ass to stir after a while.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Whats in your LC? [Re: Inocuole] 1
#23645446 - 09/14/16 06:30 PM (7 years, 4 months ago) |
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SITR is the only media I have used that produces nearly zero sediment. It's still a crappy sugary media but a bit better than karo or honey IMO. I have some colonizing right now.
What's in my LC?
Mycelium.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 4 hours
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light malt extract is very low to no sediment for me or am i doing something wrong?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Probably not. I just run my times with ME longer than I should.
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Thats why corn starch rules i run those babys with my grains jars for two hours. Its all stuck at bottom. Pasty has best answer
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 4 hours
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Re: Whats in your LC? [Re: tump]
#23645798 - 09/14/16 08:58 PM (7 years, 4 months ago) |
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wouldn't that caramelize the sugars?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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2 hours would be major overkill for grain based nutes but it won't hurt. Caramelization is less of a concern with such nute sources. Cornstarch is okay but brf suspension or grain soak water works really good. But they do need a bit longer. I would do 60 min at 15 psi with em.
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ParallelTimeStamp
Time Travler



Registered: 09/13/16
Posts: 38
Last seen: 4 years, 10 months
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Quote:
Pastywhyte said:
What's in my LC?
Mycelium.
HAHAHAHAHA! I love that answer! 
Wow! awesome I'm loving this, I set out to learn something about LC's and I have, thank you all for joining the convo and giving your two cents....I assure you to me its worth much more to me.
Who needs collage when you have a bunch of awesome people on the same "page" willing to discuss something so helpful and come to a reasonable conclusion.
I am diggin this site, lots of helpful people here, some trolls but ehh what can you do but laugh.
-------------------- "Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"
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