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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Whats in your LC? [Re: LeeHarvOz]
#23644178 - 09/14/16 09:31 AM (7 years, 4 months ago) |
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Quote:
LeeHarvOz said:
Quote:
Trusted Cultivator said: there's no essential nutrients and minerals, you would be better off using tap water. sugar LC is the worst. leftover grain soak water or malt extract work much better for LC than just Karo. YPD broth also works it's just YPD agar without the agar.
can i just take the water my rye has been soaking in to rehydrate it and sterilize it in jars with the appropriate lids to be used as LC? does anything else need to be added?
Water it down 1:2 - 1:4. Let it settle in a jar for a few hours. Decant it off the sediment if you want it to be more clear.
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ParallelTimeStamp
Time Travler



Registered: 09/13/16
Posts: 38
Last seen: 4 years, 10 months
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Quote:
Trusted Cultivator said: Water it down 1:2 - 1:4. Let it settle in a jar for a few hours. Decant it off the sediment if you want it to be more clear.
Will the sediment provide any extra food or a place for the culture to take hold, or is it useless in that sense and just waste that makes your culture non clear and be more pron to contaminates?
-------------------- "Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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The sugar water is makes it prone to contaminants.. the sediment doesn't make it more prone to anything other than clumping.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Whats in your LC? [Re: Inocuole]
#23645364 - 09/14/16 05:50 PM (7 years, 4 months ago) |
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Sediment imo isn't anything worth keeping. If it's not dissolved its just something that's hard to sterilize.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Eh, I guess if there's a lot. I've only ever had a little bit of sediment with anything. It's definitely a pain in the ass to stir after a while.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Whats in your LC? [Re: Inocuole] 1
#23645446 - 09/14/16 06:30 PM (7 years, 4 months ago) |
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SITR is the only media I have used that produces nearly zero sediment. It's still a crappy sugary media but a bit better than karo or honey IMO. I have some colonizing right now.
What's in my LC?
Mycelium.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 6 hours
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light malt extract is very low to no sediment for me or am i doing something wrong?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Probably not. I just run my times with ME longer than I should.
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Thats why corn starch rules i run those babys with my grains jars for two hours. Its all stuck at bottom. Pasty has best answer
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 6 hours
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Re: Whats in your LC? [Re: tump]
#23645798 - 09/14/16 08:58 PM (7 years, 4 months ago) |
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wouldn't that caramelize the sugars?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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2 hours would be major overkill for grain based nutes but it won't hurt. Caramelization is less of a concern with such nute sources. Cornstarch is okay but brf suspension or grain soak water works really good. But they do need a bit longer. I would do 60 min at 15 psi with em.
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ParallelTimeStamp
Time Travler



Registered: 09/13/16
Posts: 38
Last seen: 4 years, 10 months
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Quote:
Pastywhyte said:
What's in my LC?
Mycelium.
HAHAHAHAHA! I love that answer! 
Wow! awesome I'm loving this, I set out to learn something about LC's and I have, thank you all for joining the convo and giving your two cents....I assure you to me its worth much more to me.
Who needs collage when you have a bunch of awesome people on the same "page" willing to discuss something so helpful and come to a reasonable conclusion.
I am diggin this site, lots of helpful people here, some trolls but ehh what can you do but laugh.
-------------------- "Every story or tale in life came from a true source, doesnt mean that the story being told is 100% but it also doesnt mean that its not either, beware of what you get yourself into because you might find truth in a very unlikely place"
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