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Offlinetryptkaloids
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I'm a teapot


Registered: 02/08/15
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Speedy pan cyans?
    #23641432 - 09/13/16 12:28 PM (7 years, 4 months ago)

Hey guys I just got some pan cyans started on agar and I was wondering if it's possible to get enough doses for 5 people by thanksgiving for safe and sound fest.. Is this enough time for pans? Or should I get a reagent and stick with chems for speed?


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Invisiblehummingbird

Registered: 06/29/14
Posts: 2,134
Re: Speedy pan cyans? [Re: tryptkaloids]
    #23641455 - 09/13/16 12:37 PM (7 years, 4 months ago)

Pans are fast, you could have lots by Thanksgiving...especially if you already have it on agar.


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Offlinetryptkaloids
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Re: Speedy pan cyans? [Re: hummingbird]
    #23641966 - 09/13/16 03:33 PM (7 years, 4 months ago)

I hope I do. I think I may have a nice, healthy mold culture


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
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Re: Speedy pan cyans? [Re: tryptkaloids]
    #23641978 - 09/13/16 03:38 PM (7 years, 4 months ago)

Would you transfer these? 


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflinePsilosoulful

Registered: 09/05/14
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Re: Speedy pan cyans? [Re: tryptkaloids]
    #23642389 - 09/13/16 05:46 PM (7 years, 4 months ago)

Quote:

tryptkaloids said:
Would you transfer these? 



I've had pan cultures look similar to that.
Take the leading edge on the right side and transfer to a new plate.


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Offlinetryptkaloids
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Re: Speedy pan cyans? [Re: Psilosoulful]
    #23642655 - 09/13/16 06:59 PM (7 years, 4 months ago)

sloppy cuts but should be clean transfers (given this isn't harboring a contam) the dense part in the middle worries me.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflinePsilosoulful

Registered: 09/05/14
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Re: Speedy pan cyans? [Re: tryptkaloids]
    #23643841 - 09/14/16 05:23 AM (7 years, 4 months ago)

Yeah, that looks bacterial. I'd start over from spores on a clean plate.


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Offlinetryptkaloids
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Re: Speedy pan cyans? [Re: Psilosoulful]
    #23644261 - 09/14/16 10:10 AM (7 years, 4 months ago)

That's the rough mini round you see. The surface was as smooth as a babies bottom


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 9 hours
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Re: Speedy pan cyans? [Re: tryptkaloids]
    #23644269 - 09/14/16 10:15 AM (7 years, 4 months ago)

Also, why do you run from bacteria? That's kinda why we use agar.....


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleMad Season
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Registered: 09/16/12
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Re: Speedy pan cyans? [Re: tryptkaloids]
    #23644318 - 09/14/16 10:45 AM (7 years, 4 months ago)

For real. No bacteria has stopped me yet!


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