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AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 5 months
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What is Liquid Inoculate (LI)? Does it not carry the same perils as Liquid Culture (LC)?
#23638295 - 09/12/16 11:43 AM (7 years, 4 months ago) |
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I know that making LC from multispore (MS) syringes can result in contamination cause they're not always sterile.
How is LI made? And is it just as reliable as agar?
If you recommend LI, is there a specific LI tek that's superior and worthwhile?
Edited by AK1000 (09/12/16 11:46 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: What is Liquid Inoculate (LI)? Does it not carry the same perils as Liquid Culture (LC)? [Re: AK1000]
#23638378 - 09/12/16 12:19 PM (7 years, 4 months ago) |
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Quote:
AK1000 said: I know that making LC from multispore (MS) syringes can result in contamination cause they're not always sterile.
How is LI made? And is it just as reliable as agar?
If you recommend LI, is there a specific LI tek that's superior and worthwhile?
spores to LC https://www.shroomery.org/forums/showflat.php/Number/21365919#21365919 in a LC a single contaminant will grow and fuck the whole LC
LI is made from agar, agar wedge goes into sterilized water, the water is just a way to disperse the agar more evenly in your spawn.
https://www.shroomery.org/forums/dosearch.php?where=body&tosearch=main&how=all&words=liquid+inoculant&newerval=3&newertype=y&limit=25&showmain=1&sort=r&way=d
the 2nd and 5th result
https://www.shroomery.org/forums/showflat.php/Number/20429745
https://www.shroomery.org/forums/showflat.php/Number/22833314
those are the two good ones
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AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 5 months
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Re: What is Liquid Inoculate (LI)? Does it not carry the same perils as Liquid Culture (LC)? [Re: bodhisatta]
#23638528 - 09/12/16 01:39 PM (7 years, 4 months ago) |
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Thank you TC. The last link, the blenderless one, looks good. So this is technically going to be better than agar? Because it's faster? Faster than G2G and A2G due to more "inoculation points"?
One thing I'm wondering about is... how much liquid to use? Won't it mess with the moisture-content of a jar full of grains making it too wet inside? Or does a little go a long way. Thanks!
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: What is Liquid Inoculate (LI)? Does it not carry the same perils as Liquid Culture (LC)? [Re: AK1000]
#23638556 - 09/12/16 01:56 PM (7 years, 4 months ago) |
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I hate saying something is better. They are different. LI could be potentially pretty fast but so can 9 wedges dispersed into one master. Use too much LI and you have bacteria breeding grounds. Too little and it's as slow as anything else.
I like to think of liquid methods as a fast expansion and easy to inoculate large amounts without a flowhood. That's it.
I would use no more than 4 cc for a quart of grains. 2cc would be my sweet spot. More than that will be a bad thing.
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AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 5 months
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Re: What is Liquid Inoculate (LI)? Does it not carry the same perils as Liquid Culture (LC)? [Re: Pastywhyte]
#23638591 - 09/12/16 02:10 PM (7 years, 4 months ago) |
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Thanks for the help guys! I already have lids with Self-healing-injection ports (SHIPs) AND plastic covers for the quart jars so the lids and bands are one unit. I may try a batch with them. But the tic-tac-toe tiger drop is so freakin' fast I'm not sure I will bother with this... unless it's significantly faster but now I know it's an option. Thanks!
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