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OfflineMorel Guy
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Do you employ culture selection?
    #23636661 - 09/11/16 06:12 PM (7 years, 4 months ago)

What is your method of finding the best fruits?  On myc alone or do you fruit multiple potency/yeild cultures?  Be specific for our understanding. 

Strain hunting is the hardest part for me.  So many attachments, so little time.


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"in sterquiliniis invenitur in stercore invenitur"

In filth it will be found in dung it will be found


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OfflineKryptos
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Re: Do you employ culture selection? [Re: Morel Guy]
    #23637880 - 09/12/16 07:40 AM (7 years, 4 months ago)

I do half pint jars for mini-bulks, and keep the strain cultured on agar until I see yields. Best strains go from agar to LC, and best fruits from the LC (used for quarts and actual bulk) get re-cultured. For the re-culturing, I normally take a transfer from the fruit itself, as well as three plates of spores, which I then use to restart the process.


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OfflineMorel Guy
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Re: Do you employ culture selection? [Re: Kryptos]
    #23637924 - 09/12/16 08:09 AM (7 years, 4 months ago)

How do you select for quality of experience and potency.  My trips have varied so much over the years.  I guess i would figure 'I want more of this'.


--------------------
"in sterquiliniis invenitur in stercore invenitur"

In filth it will be found in dung it will be found


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OfflineKryptos
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Re: Do you employ culture selection? [Re: Morel Guy]
    #23639105 - 09/12/16 04:51 PM (7 years, 4 months ago)

I don't grow actives, but I make a lot of plates when I want to select edible cultures. I normally take the 5-6 biggest/heaviest fruits, as those tend to sell better, and plate them all (usually takes a full sleeve). Not all grow voraciously as mycelium and simultaneously produce large fruits. I assume you should be able to take a piece of a large variety of your fruits, test for potency, and then toss the weak plates.


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Invisible36fuckin5
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Registered: 08/11/03
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Re: Do you employ culture selection? [Re: Morel Guy]
    #23641428 - 09/13/16 12:27 PM (7 years, 4 months ago)

Quote:

Morel Guy said:
How do you select for quality of experience and potency.  My trips have varied so much over the years.  I guess i would figure 'I want more of this'.




Trips just vary all the time, so "quality of experience" isn't a thing you can select for. If you want a different alkaloid mix you'll need to grow another species.

As for potency, it's hard to properly breed for. You can breed for relative potency compared to whatever other samples you're fruiting out, but unless you can run chromatography on it you'll never quantify it.

So just do a lot, save them as early as you can and fruit each one, keeping good records from germination to tasting.


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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Offlinekrypto2000
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Re: Do you employ culture selection? [Re: 36fuckin5]
    #23661095 - 09/20/16 09:33 AM (7 years, 4 months ago)

I'm sure you could judge potency to a relative degree with an ehrlich reagent test. You'd have to work out a good reagent, perhaps a non standard recipe, as well as a good extraction technique so that the alkaloids are in a clear or relatively clear solution. I'm thinking something like grind .5g of mushies up, soak in 10ml of alcohol for a set amount of time (1 min?), filter, then mix with a standardized ehrlich reagent and either measure the reaction time or let sit until it has fully reacted and keep what has the most color change.


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Invisible36fuckin5
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Registered: 08/11/03
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Re: Do you employ culture selection? [Re: krypto2000]
    #23663262 - 09/20/16 09:23 PM (7 years, 4 months ago)

Quote:

krypto2000 said:
I'm sure you could judge potency to a relative degree with an ehrlich reagent test. You'd have to work out a good reagent, perhaps a non standard recipe, as well as a good extraction technique so that the alkaloids are in a clear or relatively clear solution. I'm thinking something like grind .5g of mushies up, soak in 10ml of alcohol for a set amount of time (1 min?), filter, then mix with a standardized ehrlich reagent and either measure the reaction time or let sit until it has fully reacted and keep what has the most color change.




Maybe, but I don't think it'd be very reliable. If you had a few good, potent strains (say you isolated a few PE cultures) they'd have about the same reaction.

I've always just grown them out and either eaten them myself or gave them to a friend who wants to be a guinea pig. It works well enough for how much anyone is gonna care.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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