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OfflineBIGGS22
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Why did my mini monotub fail so hard and is it possible to turn things around?
    #23632576 - 09/10/16 02:15 PM (7 years, 4 months ago)

I had one spare jar of spawn so I decided to make a mini monotub. This was my first ever attempt at growing. When the pins started coming in, I noticed they were way too thin so I upped the fae, but it doesn't seem to have had any effect. Now the veils are starting to break on the pins and things don't look very good. Any way to turn this around?

Pins arising:



Current state:




I also should mention, I skipped colonization conditions on it and did not use a casing layer. I was told this wouldn't have a huge impact on the grow.

I also recently started 2 normal sized tubs. Also skipping colonization conditions, but this time adding a casing layer. I'm now worried the same problems will arise in these tubs. Here they are at 5 days,



The tub on the left had a thinner casing layer than the one on the right.


UPDATE: Now with Trich!!



Edited by BIGGS22 (09/11/16 02:32 PM)


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23632587 - 09/10/16 02:19 PM (7 years, 4 months ago)

why skip colonizing?


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23632593 - 09/10/16 02:22 PM (7 years, 4 months ago)

I originally got the info from here,

https://www.shroomery.org/forums/showflat.php/Number/22315950

I even made my own thread on it and got even more positive feedback,

https://www.shroomery.org/forums/showflat.php/Number/23615733


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OfflineRooster Cogburn
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: mushboy]
    #23632603 - 09/10/16 02:24 PM (7 years, 4 months ago)

For your mini tub, I'm thinking not enough spawn.
The bigger tubs look fine, how much spawn was used in those?

And mushboy, a lot of here have been fruiting from spawning and it's great.


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Rooster Cogburn]
    #23632615 - 09/10/16 02:28 PM (7 years, 4 months ago)

That's very possible. I only used 1 quart of spawn, with a 1:2 ratio of spawn:coir/verm/gypsum.

The 3 quarts came out to about 2.5 inches in the tub. I know 3-4 is ideal.

The larger tubs I made with an appropriate ratio. 5:10 and 6:9 plus the casing layers on both.


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Rooster Cogburn]
    #23633389 - 09/10/16 06:13 PM (7 years, 4 months ago)

Is there any saving it or is it doomed to spawn only mini mushies?


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22] * 1
    #23633498 - 09/10/16 06:42 PM (7 years, 4 months ago)

The sub looks reaaaaaaly dry, how did you do the poly filling?


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: ghostpepper7]
    #23633525 - 09/10/16 06:50 PM (7 years, 4 months ago)

I filled the bottom holes tightly. Since it's a mini mono, I drilled 5 1/4' holes in the top of the tub. Until I noticed the thin pins, I had 3/5 holes covered with tape on each side.

I'm in a desert climate so the air has 0% humidity, but a few days ago I started keeping an ultrasonic humidifier on 24/7. I also misted it for the first time last night. I've read conflicting info on whether or not to mist monotubs, so I wasn't sure if it was a good idea. Should I start misting daily?


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23633560 - 09/10/16 07:05 PM (7 years, 4 months ago)

My tubs and this is my first tub set mind you after years of PF jars, there should always be a fine amount of mist on the sub at all times.
The tub design after months of studying the best layout of holes and sizes
6 total holes: 2 on each of the wide sides @ 1" diameter right at sub level and 1 high on the short sides (i put mine under the handles that lock the lids on) @ 1" diameter

Loose up high, tight on the bottom, used franks dial in tek
https://www.shroomery.org/forums/showflat.php/Number/17332777/fpart/1/vc/1

I do believe after my grow, I'll be casing all my tubs from now on with straight peat @ 1/4"

If your tubs stay dry like that, yes, mist! Or depending on things, you might want to dunk the tub
https://www.shroomery.org/forums/showflat.php/Number/17547374

keep us updated! I love pin porn :smile:

-Ghost


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OfflineMushierage
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: ghostpepper7] * 1
    #23633577 - 09/10/16 07:14 PM (7 years, 4 months ago)

Yeah man.  I think your stuff got dry.  Oops. :/


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23634009 - 09/10/16 09:25 PM (7 years, 4 months ago)

Thanks guys for the advice. I misted it a bit and made the walls of the tub condensation-ey.

                 
         

Do the walls of my large size mono look good?

                     
             


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23634033 - 09/10/16 09:30 PM (7 years, 4 months ago)

Wouldn't those skinny fruits be from lack of fae and lighting? How are you running your lights and are they 6500k


--------------------


Edited by Boogieman47 (09/10/16 09:31 PM)


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Offlinejust_curious
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Boogieman47]
    #23634076 - 09/10/16 09:47 PM (7 years, 4 months ago)

I have definitely noticed that the bottom holes CAN be TOO tight. Not that you shouldn't make them tight, but should't be to the point where you couldn't possibly stuff any more. That's just my opinion.

Also, I know they say that a mono is a "set and forget", but that't not always the case. You need to pay attention to your subs surface. Once you notice that it is drying up, give it a little mist. Especially in your climate. Keep in mind that evaporation does need to happen though. I agree that the skinny pins could be lack of FAE. Or, because you have no humidity, they could just be getting sucked dry and hating their little lives so they are unable to perform...if that makes sense. It does in my head, I just don't know how to explain it. BUT, at the same time, all my fruits ended up like that this time around also, but I also had crazy contams....whats going on with the black circle in your first pic? Nobody has addressed that yet. I definitely think you're going to start seeing green at the uncolonized spot if I'm seeing it corectly. That stalled/uncolonized growth tells me there a contam/bacteria there.

Good luck with your full mono's.


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: just_curious]
    #23634086 - 09/10/16 09:50 PM (7 years, 4 months ago)

I just figured it was him blocking the light for the pic


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Offlinejust_curious
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Boogieman47]
    #23634111 - 09/10/16 10:00 PM (7 years, 4 months ago)

Thats what I thought at first too, but I think its an uncolonized portion.


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Boogieman47]
    #23634118 - 09/10/16 10:02 PM (7 years, 4 months ago)

Black spot is a shadow.

I originally did stuff the bottom holes too tightly I think, there was very little dryness around them.

I forgot mention the lighting situation. At first I thought the tubs would get enough natural sunlight to grow, but since some pins were growing sideways, I had to add lighting. Right now there are 3 5000k led lights about 3-4 feet above the tubs, although they are not pointing directly down. It's a configuration like this,



I also placed an order for some 6500k led lights which should arrive in a couple days.


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23634125 - 09/10/16 10:05 PM (7 years, 4 months ago)

Ya once you fix the lights and get your poly right you should see better results what kind of poly are you using ? I don't knowif led is efficient? Cfls are what I know we use I never seen leds used before but that doesn't mean much ??


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OfflineNDStepp84
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: just_curious]
    #23634131 - 09/10/16 10:06 PM (7 years, 4 months ago)

Lol, looks more like a stone producer's fruits than cubes, definitely needs more light, 6500k CFL about a foot or less away but there may be something else going on.


--------------------

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:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23634185 - 09/10/16 10:21 PM (7 years, 4 months ago)

Did a little digging and it seems LEDs work fine, but they should be around 2,000 lumens and the ones I ordered are not.


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23634193 - 09/10/16 10:24 PM (7 years, 4 months ago)

Well some 6500k cfl are only 900 and I used those and worked fine just try them out and see before spending more dough also I ordered adapters that fit into led light fixtures for the regular screw in bulbs off eBay 5 for like 2$


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23634207 - 09/10/16 10:28 PM (7 years, 4 months ago)

Nice, the ones I ordered are 810. Hopefully they will do. There will also be 3 of them, so that might help.

Should I put some boxes below the tubs to raise them closer to the light?


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OfflineNDStepp84
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23634215 - 09/10/16 10:30 PM (7 years, 4 months ago)

I use a 7$ desk lamp or a clamp lamp with reflector, you can get them right where you want them.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
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:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23634224 - 09/10/16 10:32 PM (7 years, 4 months ago)

Mine was on the fan and I just had them on a table... https://www.amazon.com/Lithonia-Lighting-1233-SHOPLIGHT-Fluorescent/dp/B000HMBMEO/ref=sr_1_1?ie=UTF8&qid=1473568193&sr=8-1&keywords=shop+light+fixtures check this out 13$ and you can get tubes a box of 10 for 30$ each tube is supposed to good for 8 years


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Boogieman47]
    #23634227 - 09/10/16 10:33 PM (7 years, 4 months ago)

Quote:

noob47 said:
Wouldn't those skinny fruits be from lack of fae and lighting? How are you running your lights and are they 6500k



This. Also loosen the poly or something.


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Mad Season]
    #23634383 - 09/10/16 11:34 PM (7 years, 4 months ago)

Turns out the bulbs I ordered are only 250 lumens lol.

Already put in an order for these babies,
https://www.amazon.com/gp/product/B01GP08M44/ref=oh_aui_detailpage_o00_s00?ie=UTF8&psc=1

The tops of my tubs are translucent, is this an issue?



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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23635904 - 09/11/16 02:11 PM (7 years, 4 months ago)

Welp, things have only gone downhill since yesterday. A spot of trich appeared this morning and I'm not sure what to do.



I've moved my mini about 15 feet away from my monos, but now I'm freaking out thinking they've been contaminated. Should I just toss the mini and call it a day?

At lease I got these guys out of it lol,



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Offlinejust_curious
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636030 - 09/11/16 02:52 PM (7 years, 4 months ago)

Get it outside and cut out that section, maybe like an inch around it. I've cut out before when I spotted it right away and it turned out okay. Some people advise against it though. I would mist that spot lightly to keep the spores in place, cut an inch around it, and scoop out the bad spot with a spoon making sure you don't drop any. DO THIS OUTSIDE if you decide to do it...but either way, the tub needs to go out side. I had a feeling you were gonna see trich in that tub.


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OfflineNDStepp84
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636035 - 09/11/16 02:53 PM (7 years, 4 months ago)

I was afraid it kinda looked like trich pins to me but wasn't sure, what I meant by something else going on. That sucks dude, id get it out of the house dump it outside in the shade you may get something.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: NDStepp84]
    #23636053 - 09/11/16 03:00 PM (7 years, 4 months ago)

What are the chances that the other tubs have already been infected? Am I going to see trich in those too?


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636056 - 09/11/16 03:02 PM (7 years, 4 months ago)

I'm moving out of this apartment in about a month and a half so I don't mind if the surroundings are exposed to trich spores. If there's trich in the tub, does it effect the clean areas as well? Will the mushrooms that grow be edible or does trich destroy the whole thing?


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636073 - 09/11/16 03:10 PM (7 years, 4 months ago)

The other tubs should be fine if they are healthy, the fruits are fine to eat as long as they look fine.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636083 - 09/11/16 03:15 PM (7 years, 4 months ago)

I'M SO FUCKING SAD


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636118 - 09/11/16 03:25 PM (7 years, 4 months ago)

It's all part of learning man I've tossed more tubs than I have harvested but I figure out each time what I was doing wrong I been doing this 6 months and probably have another 6 till I can really eye ball the good and bad before shit goes wrong ... just take it as learning experience and go through the steps you did and try to pinpoint your flaws and read up on everything pertaining to that


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636129 - 09/11/16 03:28 PM (7 years, 4 months ago)

The infected area doesn't look too bad. Maybe it hasn't has a chance drop its disease all over the place yet. Well that's assuming that the mini mono and the 2 mono's weren't made from the same batches of grain. And as far as the mushrooms being contaminated, RR said that green mold in mushrooms isn't harmful to people. Obviously don't snort the mold spores like a line of coke. But that's also assuming that what you have in your mushrooms is green mold. But typically, you will still get a 1st flush out of a contaminated tub. But, your mushrooms look the same as my last tubs of mushrooms which tells me that the substrate is FULL of trich and it just hasn't revealed it's self yet and that the mushrooms have indeed been infected by the contaminant. I just made a thread about my mushrooms asking if people would still consume them and every single person said to go for it. Now, when you harvest them, have some sort of process of elimination for which ones to throw out and which ones to keep. But you should be good.


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: just_curious]
    #23636173 - 09/11/16 03:46 PM (7 years, 4 months ago)

It's all part of it dude, don't let it get you down. If you aren't working with agar,  start. Work on your sterile technique to produce the cleanest spawn possible.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636208 - 09/11/16 03:59 PM (7 years, 4 months ago)

I really have no right to be upset. It's my own fault for not wearing a face mask while preparing the coir. I got arrogant, thinking the HEPA filter in my appt would be enough to keep out contaminants. The same will probably happen with the other tubs. Hopefully I'll be able to get a couple trips worth out of them before it happens...


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636213 - 09/11/16 04:01 PM (7 years, 4 months ago)

I really don't think u needed a face-mask w/ coir, I used some that was sitting [opened] in a cupboard for over a yr.
Ya sure coulda used alil less fail tho ^__^


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636226 - 09/11/16 04:05 PM (7 years, 4 months ago)

kdfnmagl;dsfkmgl;ksdmfl;gdsflkg ;__;


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: rjstim]
    #23636227 - 09/11/16 04:05 PM (7 years, 4 months ago)

It's spawn and not the coir, a little RR humor "you can pick your nose while spawning to bulk" nose or ass? I can't remember.


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636230 - 09/11/16 04:07 PM (7 years, 4 months ago)

Quote:

BIGGS22 said:
I really have no right to be upset. It's my own fault for not wearing a face mask while preparing the coir. I got arrogant, thinking the HEPA filter in my appt would be enough to keep out contaminants. The same will probably happen with the other tubs. Hopefully I'll be able to get a couple trips worth out of them before it happens...



Ya this is wrong man if using coir if it contams before you flush it's your spawn 100% if using hpoo and it contams before your first flush it could be  improper pasteurising or bad spawn or both I've dropped sweat dropped cigarette ash and never wash my hands plus whatever else is floating in the air and usually is my tubs goto shit I was already thinking they would because of an iffy jar


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OfflineRooster Cogburn
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Boogieman47]
    #23636241 - 09/11/16 04:10 PM (7 years, 4 months ago)

Dude, cigarette ash? That's a funny fucking picture to imagine, not that there is anything wrong with that, but fuck yeah dude that's hardcore spawning:super:


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Rooster Cogburn]
    #23636251 - 09/11/16 04:12 PM (7 years, 4 months ago)

Well I didn't want to grab my cig with shit on my hands haha I I usually chew while spawning but I was out of redman


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Boogieman47]
    #23636271 - 09/11/16 04:17 PM (7 years, 4 months ago)

I spawn with no gloves and I've coughed and sneezed into a tub I'm spawning more often than not.


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OfflineRooster Cogburn
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Mad Season]
    #23636276 - 09/11/16 04:19 PM (7 years, 4 months ago)

I often drop beads of sweat on to the casing while Im trimming off the liner.


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636285 - 09/11/16 04:23 PM (7 years, 4 months ago)

Damn, I was so careful when I inoculated my spawn jars though. I cleaned my whole apartment, sprayed lysol everywhere, showered, facemask, gloves... the works. I wonder where it went wrong.


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Rooster Cogburn]
    #23636292 - 09/11/16 04:25 PM (7 years, 4 months ago)

I hate trimming the liner I've made a few recent tubs without a liner... mad doesn't use a liner he's got the voodoo where he gets no side pins haha... knocking up with a syringe without a doubt man


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636294 - 09/11/16 04:25 PM (7 years, 4 months ago)

Syringes aren't clean, use agar in a SAB, practice your procedures, don't reach over your work ect.

If problems still go over your filters, up your PC time keep troubleshooting eliminating one vector at a time.


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:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: NDStepp84]
    #23636318 - 09/11/16 04:34 PM (7 years, 4 months ago)

:whathesaid: always have some uninoculated control jars to make sure your filters are good. Up the PC times to at least 2 hours, and get a SAB and do agar. I can dig around and show you what spore syringes look like if you want lol!


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Mad Season]
    #23636403 - 09/11/16 05:00 PM (7 years, 4 months ago)

Damn I saw them pins and thought "ain't no way those are from poor FAE." Classic trich pins.


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Pastywhyte]
    #23636422 - 09/11/16 05:03 PM (7 years, 4 months ago)

Quote:

Pastywhyte said:
Damn I saw them pins and thought "ain't no way those are from poor FAE." Classic trich pins.



So true tho. I knew something was up. I shouldn't have second guessed myself haha. They didn't have excessive fuzzy feet and the caps looked big enough for their actual size. Fuckin skinny trich pins lol


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636455 - 09/11/16 05:12 PM (7 years, 4 months ago)

Quote:

BIGGS22 said:
Damn, I was so careful when I inoculated my spawn jars though. I cleaned my whole apartment, sprayed lysol everywhere, showered, facemask, gloves... the works. I wonder where it went wrong.



It sounds like you meant well with the precautions, but did you flame sterilize the needle before each injection? Also did you swab the injection ports with isopropyl immediately before? Those are a couple things I've always done. It is riskier with MS and grains, but it is still possible.


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: rjstim]
    #23636596 - 09/11/16 05:52 PM (7 years, 4 months ago)

Yea, I flame sterilized, but I did not swab with isopropyl. I didn't even know that was necessary.

The trich pins is also news to me. I read a few threads on thin pins and none of them mentioned anything about trich causing them.


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636608 - 09/11/16 05:55 PM (7 years, 4 months ago)

No wonder why agar is such a big part of growing. I always thought it was because it saves money on syringes lol. Had no idea it was more sterile.


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636617 - 09/11/16 06:00 PM (7 years, 4 months ago)

Agar forces the culture to grow in 2 dimensions where you can identify and transfer away from contams, ensuring you have a clean inoculant. Several other benefits like cloning and growing countless grows from a single drop, or using a swab or loop to grow from your own prints, storing live cultures and proven genetics for years and so on good stuff:thumbup:


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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
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:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: NDStepp84]
    #23636637 - 09/11/16 06:06 PM (7 years, 4 months ago)



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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: NDStepp84]
    #23636639 - 09/11/16 06:06 PM (7 years, 4 months ago)

Yeah, working with agar is a sure fire way to get clean inoculate. Especially if you do repeated transfers of high performing strains. You should look into isolating. That way you can get some "cream of the crop" fruits. And, it was all under your control. I wish I would have done that when I first started. I started with agar immediately, but I wish I would have know about isolating, different agar recipes, do's and don'ts. Instead, I let my agar grow and inoculated via "tiger drop" (something else for you to know).

Check out
Agar
Pasty plates
Tiger drop
Isolating
Slants
Cloning

And then have another go at it


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: just_curious]
    #23636652 - 09/11/16 06:10 PM (7 years, 4 months ago)

Cloning is cool but isolating is tedious as fuck. The time and effort invested is not worth it unless you are doing it as a side project and don't care about how long it takes. Get 20 clones and keep the best one. Have some fruits in the meantime.


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Pastywhyte]
    #23636762 - 09/11/16 06:48 PM (7 years, 4 months ago)

....that's what I meant lol. Ot is good information to know though. I figured you as an isolating guy Pasty. So you just took a bunch of clones, expanded them while keeping a master, and did a test run with all your other clones in order to find the best one, and then when you found it, you went back to the master to create more?

Also, maybe you can/will answer this for me Pasty...why isn't senescence an issue when it comes to working with agar and isolating like it is when you do a G2G. I've read some accounts of people using upwards of 20-30 plates before they got their isolate perfect.


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: just_curious]
    #23636817 - 09/11/16 07:05 PM (7 years, 4 months ago)

It is an issue. I am careful to keep my master cultures from growing out too far between slants. Obviously a true isolate will live longer than a clone but a clone can still last a long fucking time.

I do have a true isolate. It's a PE that produces terrible yields but can drop spores. But my best clones are still worthy producers, my RW F2 clone will dump out 10+ dry oz per 66 quart tub on the first flush.


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636821 - 09/11/16 07:07 PM (7 years, 4 months ago)

So what are the tubs going to look like if they are triched out? Will I be able to get more than a few grams of mushies out of it?


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636902 - 09/11/16 07:30 PM (7 years, 4 months ago)

Is it possible it was insufficient lighting and not trich that caused the mushrooms to grow so small?

I was looking through this thread,
https://www.shroomery.org/forums/showflat.php/Number/17231150

Take a look at Twistedty's over misted and insufficient light pic. They look pretty similar to mine. Since I added better lighting, do you think the pins will come in fatter in the tubs?


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Offlinejust_curious
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636926 - 09/11/16 07:42 PM (7 years, 4 months ago)

Quote:

BIGGS22 said:
So what are the tubs going to look like if they are triched out? Will I be able to get more than a few grams of mushies out of it?




You will probably get a first flsuh...maybe an ounce?

Quote:

BIGGS22 said:
Is it possible it was insufficient lighting and not trich that caused the mushrooms to grow so small?

I was looking through this thread,
https://www.shroomery.org/forums/showflat.php/Number/17231150


Take a look at Twistedty's over misted and insufficient light pic. They look pretty similar to mine. Since I added better lighting, do you think the pins will come in fatter in the tubs?




Definitely the trich


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636934 - 09/11/16 07:45 PM (7 years, 4 months ago)

Well that's good to hear. One ounce is far more than I need.


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OfflineBIGGS22
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636941 - 09/11/16 07:48 PM (7 years, 4 months ago)

Thanks for all the advice everyone! I'll update with the status of the tubs in a week or so.


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OfflinePsilosoulful

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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636982 - 09/11/16 08:05 PM (7 years, 4 months ago)

:goodluck:


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Invisibledankington
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Psilosoulful]
    #23636990 - 09/11/16 08:07 PM (7 years, 4 months ago)

An ounce?? I hope so, man.


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: dankington]
    #23637118 - 09/11/16 08:42 PM (7 years, 4 months ago)

I just pulled what I'm assuming is going to be an ounce of my tub and it was WAAAY worse than his. I have hope for him.


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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: just_curious]
    #23637139 - 09/11/16 08:48 PM (7 years, 4 months ago)

I need like fortyhundred ounces a week LMAO


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