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OfflineRooster Cogburn
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Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Boogieman47]
    #23636241 - 09/11/16 04:10 PM (7 years, 4 months ago)

Dude, cigarette ash? That's a funny fucking picture to imagine, not that there is anything wrong with that, but fuck yeah dude that's hardcore spawning:super:


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InvisibleBoogieman47
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Registered: 03/05/16
Posts: 9,712
Loc: Under your bed Flag
Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Rooster Cogburn]
    #23636251 - 09/11/16 04:12 PM (7 years, 4 months ago)

Well I didn't want to grab my cig with shit on my hands haha I I usually chew while spawning but I was out of redman


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InvisibleMad Season
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Registered: 09/16/12
Posts: 12,666
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Boogieman47]
    #23636271 - 09/11/16 04:17 PM (7 years, 4 months ago)

I spawn with no gloves and I've coughed and sneezed into a tub I'm spawning more often than not.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineRooster Cogburn
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Registered: 07/19/16
Posts: 265
Last seen: 7 years, 2 months
Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Mad Season]
    #23636276 - 09/11/16 04:19 PM (7 years, 4 months ago)

I often drop beads of sweat on to the casing while Im trimming off the liner.


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OfflineBIGGS22
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Registered: 07/28/16
Posts: 82
Last seen: 10 months, 28 days
Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636285 - 09/11/16 04:23 PM (7 years, 4 months ago)

Damn, I was so careful when I inoculated my spawn jars though. I cleaned my whole apartment, sprayed lysol everywhere, showered, facemask, gloves... the works. I wonder where it went wrong.


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InvisibleBoogieman47
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Registered: 03/05/16
Posts: 9,712
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Rooster Cogburn]
    #23636292 - 09/11/16 04:25 PM (7 years, 4 months ago)

I hate trimming the liner I've made a few recent tubs without a liner... mad doesn't use a liner he's got the voodoo where he gets no side pins haha... knocking up with a syringe without a doubt man


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OfflineNDStepp84
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Registered: 04/23/15
Posts: 4,956
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636294 - 09/11/16 04:25 PM (7 years, 4 months ago)

Syringes aren't clean, use agar in a SAB, practice your procedures, don't reach over your work ect.

If problems still go over your filters, up your PC time keep troubleshooting eliminating one vector at a time.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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InvisibleMad Season
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Registered: 09/16/12
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: NDStepp84]
    #23636318 - 09/11/16 04:34 PM (7 years, 4 months ago)

:whathesaid: always have some uninoculated control jars to make sure your filters are good. Up the PC times to at least 2 hours, and get a SAB and do agar. I can dig around and show you what spore syringes look like if you want lol!


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
Posts: 37,810
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Mad Season]
    #23636403 - 09/11/16 05:00 PM (7 years, 4 months ago)

Damn I saw them pins and thought "ain't no way those are from poor FAE." Classic trich pins.


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InvisibleMad Season
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Pastywhyte]
    #23636422 - 09/11/16 05:03 PM (7 years, 4 months ago)

Quote:

Pastywhyte said:
Damn I saw them pins and thought "ain't no way those are from poor FAE." Classic trich pins.



So true tho. I knew something was up. I shouldn't have second guessed myself haha. They didn't have excessive fuzzy feet and the caps looked big enough for their actual size. Fuckin skinny trich pins lol


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisiblerjstim
Stranger

Registered: 09/09/16
Posts: 103
Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636455 - 09/11/16 05:12 PM (7 years, 4 months ago)

Quote:

BIGGS22 said:
Damn, I was so careful when I inoculated my spawn jars though. I cleaned my whole apartment, sprayed lysol everywhere, showered, facemask, gloves... the works. I wonder where it went wrong.



It sounds like you meant well with the precautions, but did you flame sterilize the needle before each injection? Also did you swab the injection ports with isopropyl immediately before? Those are a couple things I've always done. It is riskier with MS and grains, but it is still possible.


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OfflineBIGGS22
Stranger


Registered: 07/28/16
Posts: 82
Last seen: 10 months, 28 days
Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: rjstim]
    #23636596 - 09/11/16 05:52 PM (7 years, 4 months ago)

Yea, I flame sterilized, but I did not swab with isopropyl. I didn't even know that was necessary.

The trich pins is also news to me. I read a few threads on thin pins and none of them mentioned anything about trich causing them.


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OfflineBIGGS22
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Registered: 07/28/16
Posts: 82
Last seen: 10 months, 28 days
Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636608 - 09/11/16 05:55 PM (7 years, 4 months ago)

No wonder why agar is such a big part of growing. I always thought it was because it saves money on syringes lol. Had no idea it was more sterile.


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OfflineNDStepp84
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Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636617 - 09/11/16 06:00 PM (7 years, 4 months ago)

Agar forces the culture to grow in 2 dimensions where you can identify and transfer away from contams, ensuring you have a clean inoculant. Several other benefits like cloning and growing countless grows from a single drop, or using a swab or loop to grow from your own prints, storing live cultures and proven genetics for years and so on good stuff:thumbup:


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: NDStepp84]
    #23636637 - 09/11/16 06:06 PM (7 years, 4 months ago)



--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinejust_curious
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Registered: 03/15/14
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Loc: 'Merica (more spcifically...
Last seen: 1 year, 6 months
Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: NDStepp84]
    #23636639 - 09/11/16 06:06 PM (7 years, 4 months ago)

Yeah, working with agar is a sure fire way to get clean inoculate. Especially if you do repeated transfers of high performing strains. You should look into isolating. That way you can get some "cream of the crop" fruits. And, it was all under your control. I wish I would have done that when I first started. I started with agar immediately, but I wish I would have know about isolating, different agar recipes, do's and don'ts. Instead, I let my agar grow and inoculated via "tiger drop" (something else for you to know).

Check out
Agar
Pasty plates
Tiger drop
Isolating
Slants
Cloning

And then have another go at it


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
Posts: 37,810
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: just_curious]
    #23636652 - 09/11/16 06:10 PM (7 years, 4 months ago)

Cloning is cool but isolating is tedious as fuck. The time and effort invested is not worth it unless you are doing it as a side project and don't care about how long it takes. Get 20 clones and keep the best one. Have some fruits in the meantime.


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Offlinejust_curious
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: Pastywhyte]
    #23636762 - 09/11/16 06:48 PM (7 years, 4 months ago)

....that's what I meant lol. Ot is good information to know though. I figured you as an isolating guy Pasty. So you just took a bunch of clones, expanded them while keeping a master, and did a test run with all your other clones in order to find the best one, and then when you found it, you went back to the master to create more?

Also, maybe you can/will answer this for me Pasty...why isn't senescence an issue when it comes to working with agar and isolating like it is when you do a G2G. I've read some accounts of people using upwards of 20-30 plates before they got their isolate perfect.


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: just_curious]
    #23636817 - 09/11/16 07:05 PM (7 years, 4 months ago)

It is an issue. I am careful to keep my master cultures from growing out too far between slants. Obviously a true isolate will live longer than a clone but a clone can still last a long fucking time.

I do have a true isolate. It's a PE that produces terrible yields but can drop spores. But my best clones are still worthy producers, my RW F2 clone will dump out 10+ dry oz per 66 quart tub on the first flush.


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OfflineBIGGS22
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Registered: 07/28/16
Posts: 82
Last seen: 10 months, 28 days
Re: Why did my mini monotub fail so hard and is it possible to turn things around? [Re: BIGGS22]
    #23636821 - 09/11/16 07:07 PM (7 years, 4 months ago)

So what are the tubs going to look like if they are triched out? Will I be able to get more than a few grams of mushies out of it?


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