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Invisibler.lutece
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Parafilm vs. Saran Wrap for Long-Term Storage of Sterile Water Suspensions
    #23629570 - 09/09/16 04:10 PM (7 years, 4 months ago)

I know the Parafilm vs Saran Wrap debate has been driven well into the ground, but I'm interested in knowing if anyone has used Saran Wrap instead of Parafilm for long-term culture storage, specifically as a sterile water suspension.

Benefits of Parafilm over SW mostly boil down to its breathability, particularly when incubating plates and storing live cultures. However, I'm sure most of us have read the posting about storing mycelium in stasis in sterile, distilled water (see this post for more details). Would there be any benefits to using Parafilm with this method instead of Saran Wrap, outside of already having Parafilm available for your grow in a more manageable size? The purpose of the Parafilm in this technique is to prevent evaporation, however Saran Wrap does that job perfectly well.

This is mostly a curiosity; as Parafilm should already be available to us by the time we're storing cultures and the amount to seal a culture is so small, it's not necessarily worth replacing.


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One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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OfflineNooner
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Registered: 06/15/14
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Re: Parafilm vs. Saran Wrap for Long-Term Storage of Sterile Water Suspensions [Re: r.lutece]
    #23629767 - 09/09/16 04:55 PM (7 years, 4 months ago)

If you know the debate then you know the answer; use either one.


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