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OfflineVTsal
Stranger
Registered: 05/22/16
Posts: 51
Last seen: 2 years, 6 months
Two quick questions
    #23628956 - 09/09/16 12:53 PM (7 years, 5 months ago)

Just a couple of basic questions -

How long can you wait to use a quart grain jar after PCing it?

Same with agar - how long can you keep a petri dish with agar before using it?  How would you store it?  Seal it with Cling Wrap or not?

It seems to make sense to run the PC full even if I'm not going to use everything right away.  Am I wrong?

Thanks

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Offlinejhoppa
mushy musician


Registered: 06/08/08
Posts: 242
Last seen: 6 years, 8 months
Re: Two quick questions [Re: VTsal]
    #23629362 - 09/09/16 03:05 PM (7 years, 5 months ago)

I would only PC what you expect to need within the next few days. Sterilization/filters/whatever isn't 100% effective against contaminants, especially in a home setting; when you sterilize, you're really just starting a clock that lets your desired culture take a strong hold before anything else can. Let it sit long enough and other stuff will start to grow. Yeah, there's a solid window where most everything inside the jar is dead, but why play chicken? It just takes a couple hours at most to PC a new set of stuff; is two hours of running your stove really worth risking a crop over?

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OfflineVTsal
Stranger
Registered: 05/22/16
Posts: 51
Last seen: 2 years, 6 months
Re: Two quick questions [Re: jhoppa]
    #23630630 - 09/09/16 08:59 PM (7 years, 5 months ago)

Yeah.  I see your point.  Thanks.

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