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OfflinePieEyed-Messiah
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First agar trial
    #23628344 - 09/09/16 08:19 AM (7 years, 5 months ago)

This may sound stupid butt here it goes after miserably failing at (5) lc's I decided to try and use the last bit of my spores on agar to just see if the syringe was viable now 1st I think I put too much spore solution in each dish  causing many  blooms 2nd there is alot of mycelium starting to show  how do I diffrensiate mycelium from contams? on agar is there anything besides spider mold that could be mistaken as mycelium? These look fine to me but the 5 failing lc's scared me

Edited by PieEyed-Messiah (09/09/16 08:29 AM)

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OfflinePsilocyBen17
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Re: First agar trial [Re: PieEyed-Messiah]
    #23628376 - 09/09/16 08:37 AM (7 years, 5 months ago)

Lookin good man. Transfer that to a new plate as soon as you can. Agars easier than people make it out to be eh?


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Offlinespore-ty
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Re: First agar trial [Re: PsilocyBen17]
    #23628571 - 09/09/16 10:04 AM (7 years, 5 months ago)

Quote:

PsilocyBen17 said:
Lookin good man. Transfer that to a new plate as soon as you can. Agars easier than people make it out to be eh?






Can i steam sterilize agar media?

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Invisibleamidogen
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Re: First agar trial [Re: spore-ty]
    #23628879 - 09/09/16 12:15 PM (7 years, 5 months ago)

.


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The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (04/19/18 09:43 AM)

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Offlinespore-ty
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Re: First agar trial [Re: amidogen]
    #23628903 - 09/09/16 12:26 PM (7 years, 5 months ago)

So are you sayig steaming will only kill a certain kind of contams? I ask because i saw this

If you don't have a pressure cooker agar media can be sterilized by steam alone, steam your agar bottle like you would a PF jar for no less than 45m). Not having a PC/autoclave is no reason to avoid agar.

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Offlineenlightenment
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Re: First agar trial [Re: spore-ty]
    #23628922 - 09/09/16 12:36 PM (7 years, 5 months ago)

I have seen people cooking agar in an Erlenmyer flask on the stove. I think some of them used antibiotics. If i remember correctly there is a video on youtube where someone is cooking the agar on the stove or a heating plate.

//edit

found the video:




But I personally would never do agar this way. I feel a lot safer using an autoclave.

Edited by enlightenment (09/09/16 12:40 PM)

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Invisibleamidogen
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Re: First agar trial [Re: spore-ty]
    #23629461 - 09/09/16 03:39 PM (7 years, 5 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (04/19/18 09:44 AM)

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OfflinePieEyed-Messiah
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Re: First agar trial [Re: PsilocyBen17]
    #23630149 - 09/09/16 06:32 PM (7 years, 5 months ago)

Quote:

PsilocyBen17 said:
Lookin good man. Transfer that to a new plate as soon as you can. Agars easier than people make it out to be eh?



Why wold I transfer it asap wouldn't it be better to let it colonize a little more? I do plan on transferring. My plan is to inoc a bunch of premed pasty plates and do some tiger drop into wbs.soo that being said shouldn't I maximize the amount of mycelium to be used? By letting it grow?

Edited by PieEyed-Messiah (09/09/16 06:37 PM)

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OfflinePieEyed-Messiah
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Re: First agar trial [Re: spore-ty]
    #23630180 - 09/09/16 06:38 PM (7 years, 5 months ago)

Quote:

spore-ty said:
Quote:

PsilocyBen17 said:
Lookin good man. Transfer that to a new plate as soon as you can. Agars easier than people make it out to be eh?






Can i steam sterilize agar media?



Thx for jacking my thread try posting your own ehhh?


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OfflinePsilocyBen17
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Re: First agar trial [Re: PieEyed-Messiah]
    #23630745 - 09/09/16 09:43 PM (7 years, 5 months ago)

Quote:

PieEyed-Messiah said:
Quote:

PsilocyBen17 said:
Lookin good man. Transfer that to a new plate as soon as you can. Agars easier than people make it out to be eh?



Why wold I transfer it asap wouldn't it be better to let it colonize a little more? I do plan on transferring. My plan is to inoc a bunch of premed pasty plates and do some tiger drop into wbs.soo that being said shouldn't I maximize the amount of mycelium to be used? By letting it grow?




Yes sir. Sounds like you know what you are doing :thumbup:

That being said spores are very dirty....its best to do at least one transfer before you do the tiger drop just to be SURE that its clean. Even if it looks clean there can be benign contams hiding in there....best to use an extra $0.10 of agar instead of wasting $1 of grain and all the cleaning involved.  Thats my thinking anyways but i was growing commercially for a while.

By doing this you will see a 99.9% success rate in no time. :cool:


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