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InvisibleBoogieman47
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what do you vets think of this idea as a tek??
    #23627073 - 09/08/16 08:05 PM (7 years, 4 months ago)

OK so I was thinking on making some penis swabs and I have test tubes... what if I took a regular qtip and put them in the tubes with 5ml water with syringes in foil filled with 5ml PC for 35 minutes then take my swabs of the pe I want in front of the flowhood and put the swab back into the tube close the cap add the water from syringe shake then draw out my spore solution ?


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OfflineX2QE
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Re: what do you vets think of this idea as a tek?? [Re: Boogieman47]
    #23627147 - 09/08/16 08:23 PM (7 years, 4 months ago)

I think the water in the syringe would boil out. Wouldn't it be better to take your swabs to agar, clean, then make liquid inoculant?


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InvisibleBoogieman47
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Re: what do you vets think of this idea as a tek?? [Re: X2QE]
    #23627170 - 09/08/16 08:33 PM (7 years, 4 months ago)

I don't think it will boil out people do it alot maybe only being 5ml IDK but reason being making a spore syringe is that it stores longer


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Invisible36fuckin5
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Re: what do you vets think of this idea as a tek?? [Re: Boogieman47]
    #23627452 - 09/08/16 10:23 PM (7 years, 4 months ago)

A spore syringe does not store longer than a swab. Dry spores always last longer.


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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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InvisibleBoogieman47
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Re: what do you vets think of this idea as a tek?? [Re: 36fuckin5]
    #23627461 - 09/08/16 10:26 PM (7 years, 4 months ago)

I meant as for the li but would that work to make spore syringes? And what if I threw like 10 qtips into a dry test tube in the PC would that be good to collect spores?


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Invisible36fuckin5
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Re: what do you vets think of this idea as a tek?? [Re: Boogieman47]
    #23627521 - 09/08/16 10:51 PM (7 years, 4 months ago)

I would individually wrap them. You don't want anything exposed, even in front of the hood for longer than you absolutely have to.

I've had syringes boil out when trying to PC water in them. It's just not the best practice. Swabs also tend to be pretty dirty since you have to touch the gills with it. I would highly suggest just putting a swab to agar.


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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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InvisibleBoogieman47
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Re: what do you vets think of this idea as a tek?? [Re: 36fuckin5]
    #23627540 - 09/08/16 10:59 PM (7 years, 4 months ago)

That makes sense but prints can be dirty also right? I saw one dude said to take three prints off each cap to get cleaner prints but how do people get pe syringes?


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Invisiblefilthyknees
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Re: what do you vets think of this idea as a tek?? [Re: Boogieman47]
    #23628460 - 09/09/16 09:21 AM (7 years, 4 months ago)

Quote:

noob47 said:
how do people get pe syringes?



I wondered this yesterday while taking peu swabs. Anybody make pe syringes before?


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InvisibleMad Season
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Re: what do you vets think of this idea as a tek?? [Re: filthyknees]
    #23628496 - 09/09/16 09:36 AM (7 years, 4 months ago)

Vendors do it some special way involving centrifuges apparently. The way I do it is hella ghetto compared to that lol. I take a sterile sandwich bag, add sterile water, hold the swab into the water by holding it on the outside of the bag, and massage the swab in the water through the bag with your free hand. Suck up the solution in syringes. 1 black swab makes 4-8 syringes no problem


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Invisible36fuckin5
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Re: what do you vets think of this idea as a tek?? [Re: Mad Season]
    #23628712 - 09/09/16 11:07 AM (7 years, 4 months ago)

Any spores can be dirty. It's really not reccomended to go straight from spores to grain, be it in liquid or not. Agar is always my first step. It's better to take an extra week then to wait 6 just to find out that your culture wasn't as clean as you thought.


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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineNDStepp84
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Re: what do you vets think of this idea as a tek?? [Re: 36fuckin5]
    #23628808 - 09/09/16 11:52 AM (7 years, 4 months ago)

For swabs I cut one end off a bunch of q-tips(synthetic is better than cotton if you can find them) individually wrap each one in foil, place in a pint jar with a sfd lid and PC. When swabbing a fruit, do so in a SAB and let dry then wrap back up.


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InvisibleNumeroEno
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Re: what do you vets think of this idea as a tek?? [Re: NDStepp84]
    #23628828 - 09/09/16 11:59 AM (7 years, 4 months ago)

If you really want a PE syringe, A good way to do it is to PC a jar of water and a shotglass, draw up the sterile water with a fresh sterile syringe, take a mushroom with an unbroken veil, break the veil with a sterile scalpel blade, hold the mushroom over the shotglass, and squirt the sterile water over the gills so the water ends up in the shotglass. Then draw the water back up in the syringe and it will have spores. IMO just taking a sterile swab is much easier.


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InvisibleBoogieman47
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Re: what do you vets think of this idea as a tek?? [Re: 36fuckin5]
    #23628962 - 09/09/16 12:54 PM (7 years, 4 months ago)

Quote:

36fuckin5 said:
Any spores can be dirty. It's really not reccomended to go straight from spores to grain, be it in liquid or not. Agar is always my first step. It's better to take an extra week then to wait 6 just to find out that your culture wasn't as clean as you thought.



Ya all I do is agar also man I tried one time with a syringe and I didn't even get growth so I never tried again thesame syringe worked on agar I just was thinking of a way to make pe syringes ... those are pretty slick ideas for making syringes and swabs thanks for the input everyone


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