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OfflineSteward
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Tropical Outdoor Fruiting House/Indoor Spawning Chamber
    #23626156 - 09/08/16 03:14 PM (7 years, 4 months ago)

Hello. I am currently in the process of starting up a small mushroom operation and wanted to bounce some ideas against like-minds... Currently, I have the materials to construct a small flow hood and cultivation supplies: bags, tubes, pressure cooker, etc. So I am ready on that front.

On the other hand... I have a 10'x20' steel galvanized pipe frame/fittings that I think I will outfit with a heavy shade cloth to serve as a fruiting house. Temps can swing over 85 on any given day so keeping it cool will be the theme here... I've found a material called aluminet that can serve as a shade cloth and not collect heat like the typical black shade cloths being used. I will get some fans in there as well, how to most effectively utilize them is up in the air for me.

I have half a garage to dedicate to spawning and incubation, the problem is one side of the garage Is slatted and open to the outside. I'm conflicted about whether or not I should build a simple wooden frame and outfit it with plastic or if there is another solution that eludes me.

Before I forget I'm aiming to grow oysters. My state has strict regulations on the importation of microorganisms and therefore I am only permitted to import pleurotus ostreatus, pleurotus djamor, and shiitake (at the moment). I believe I would be able to get permitted to import pleurotus cystidiosus and pleurotus sajor-caju as well.

I'll document my progress and update with pictures when there is something to take pictures of. Thank you for your thoughts, comments, criticism.


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OfflineTravelAgency
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Steward]
    #23634909 - 09/11/16 07:49 AM (7 years, 4 months ago)

Check out Gr0wer's Garage Grow Room Build and his YouTube channel.

Keep us updated.

:threadmonitor:


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OfflineSteward
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: TravelAgency]
    #23637456 - 09/12/16 12:24 AM (7 years, 4 months ago)

Thanks for the pointers, friend. Gr0wer has some great info documented~

Started building the flowhood today, probably wont get to finish til the end of the week due to time constraints. 3/4" maple plywood is heavy but it was free so it works great, it's nice to look at as well. I cut my boards today and lathered them up with polyurethane and they are currently drying... Will give them a fast sanding before i assemble it all.

I'll also get to building the small flowhood room, simple wooden frame/plastic and a zipper/velcro door as stated before. Getting there...


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OfflineSteward
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Steward]
    #23667650 - 09/22/16 10:44 AM (7 years, 4 months ago)

Got all my materials, finished flow hood late last night and I need to slap together my clean room. Since the spawn showed up yesterday as well (before expected shipment date) I will be spawning aloha medicinals ElmA  Pleurotus ostreatus into unsupplemented hardwood fuel pellets (oak/mesquite) in filtered bags via Lipa tek. I will inoculate in front of the flow hood as well as take some slants.

This is my first hand at any of this... I'm hoping round 2 will be sterilized, supplemented and on local hardwood sawdust. Will bring back some pics later today/tomorrow. :mushroom2:


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OfflineFerather
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Steward]
    #23667675 - 09/22/16 10:58 AM (7 years, 4 months ago)

Good luck from Ferather!    :thumbup:


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OfflineTravelAgency
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Ferather]
    #23671398 - 09/23/16 03:22 PM (7 years, 4 months ago)

Sounds like a plan mate! Document everything!

:popcorn:


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OfflineSteward
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: TravelAgency]
    #23672865 - 09/24/16 01:48 AM (7 years, 4 months ago)

Thanks~

I had only 40 lbs of HWFP(dry), which is all inoculated at this point. I have some leftover spawn, hope it keeps another day and I'll probably get more HWFP.

Here's a pic of my temporary setup(bedroom), need to get a prefilter on there and stop putting things next to the intake lol...:


One of my bags 24hrs after inoculation:


Media felt a tad dry, but I chose to err on the side of dry rather than wet. I went with 1:1 HWFP:water with 6 cups of HWFP per block and my spawn rates are kind of sporadic (aimed for 1/2L per block). I also made 2x 8 cup blocks and 2x 10 cup blocks just out of curiosity. It was difficult with that 10lb bag of spawn and my small hood, not to mention I'm not very good at guesstimating. 14 bags total, if I remember correctly.

It was fun to work in front of the hood as well, I caught myself in small errors (nearly forgetting to flame sterilize between cultures, opening lids toward hood, etc.) which was good and I think I will have a good flow with a little more experience.

Need to build my clean room, wrap my greenhouse/shadehouse structure with shadecloth and secure a local source for substrate. Aloha~


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PEACE BE DA JOURNEY


Edited by Steward (09/24/16 11:58 AM)


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OfflineSteward
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Steward]
    #23690414 - 09/29/16 12:31 PM (7 years, 3 months ago)

Colonizing right along:


Bottom shelf was inoculated first, then the next two shelves were inoculated the day after and the following day. Spawn rates were very different and a lot of blocks are colonized already with some lagging behind (due to spawn rates).

I bought some shrooms from a market and took tissue to Petri dishes, got 1 shiitake that I'll use if I can get good growth from and 1 button mushroom dish that was kind of just for shits and giggles. Both have recovered and are growing~

Going to pick up warm weather shiitake from field and forest as well as their pleurotus djamor. Will be testing the 3 shiitake varieties next round... Which I'm gathering supplies for. Aloha for now~


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OfflineSteward
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Steward]
    #23719508 - 10/08/16 01:25 PM (7 years, 3 months ago)



17/18 blocks fruiting outside, they all pinned in the bag. I removed the bags entirely for some but found that it was tearing large chunks of substrate off the bottom of the blocks, so I left the bottom of the bags/cut the tops off the rest. Need to get up the rest of my shadecloth and I've some transparent plastic I'll use to maintain humidity. A lot of the time when it's raining its 90%+ humid and I don't need to worry but it can dry up quickly around here.



First bunch from the smallest block forced into fruiting early, texture raw was like tofu... Seared in a hot pan with some butter, they developed a nutty/earthy taste and were tender with little "chew". Great with a ribeye and pesto pasta~

Looks like I lost a bunch of pins while I'm still trying to get their fruiting environment setup, not a huge deal. Some of the caps have stripes/striations, thinking the times that they got wet they didn't dry off quick enough.



Spawn room needs a plastic roof and it's ready to get nuked with cleaning solution~ Simple 4'x6' room should serve me well as I plan to fill my shadehouse with blocks and logs alike. I'm going to be testing local/invasive species woods for their viability as substrate as well. I want to ditch the spawn bags but I imagine bottles being a headache...


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PEACE BE DA JOURNEY


Edited by Steward (10/08/16 01:34 PM)


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OfflineSteward
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Steward]
    #23734018 - 10/13/16 01:02 PM (7 years, 3 months ago)

I came away with around 4 edible lbs total from these 18 blocks on the first flush. It has been a sobering learning experience but I had more fun than anything actually getting my feet wet for the first time.

I left a plastic covering on my shelf to protect them from the rain and forgot to take it off. Needless to say a few blocks have contaminants growing on them from the severe lack of FAE. I've also learned I need to step up my shadehouse... Temps hit 93F in the heat of the day, at times (due to lack of covering) they were exposed to direct sunlight. I lost probably another pound of good mushies to the heat.

I have 73% shadecloth currently, will look into 90-95%. Then finish covering the entire structure with shade... I'll get a fan or two in there...  I'd like to clean the spot up before I snap a pic, won't be able to for a few days. Blocks are partially covered by plastic in the shade.


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PEACE BE DA JOURNEY


Edited by Steward (10/13/16 01:03 PM)


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OfflineSteward
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Steward]
    #23742503 - 10/16/16 01:07 PM (7 years, 3 months ago)

Thinking for my next round I'll force pinning by poking holes in my bags, attempting to encourage them to form larger clusters and making harvest easier. This round all pinned in the bag before the bags were stripped and the pins were numerous and scattered. I won't be working with bags bigger than 5-6lbs, just seems to be the sweet spot.

Need to revamp my shadehouse when I get back to it. Sorry for boring anyone, this is a good note pad.


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OfflineSteward
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Steward]
    #23766562 - 10/24/16 11:55 AM (7 years, 3 months ago)

Flush #2 is underway, despite some contams on the blocks. Will snag some pictures soon.

What really concerns me is how terrible my agar work is. Despite my flow hood, clean room and "sterile technique" I managed to contaminate an entire sleeve of Petri dish, 4 of them had my ElmA master culture transferred to them. All of them (after a week) showed whitish, round satellite colonies.
The room was sprayed with bleach and left closed with the hood running. Every tool and surface were wiped down with 70% alcohol and, of course, the scalpel was flame sterilized. After I got back in and began wiping my hands, I nearly fell and got a piece of paper towel right into my blowers intake. I'm hoping and praying that remains from the paper towel wont obstruct my hepa( I may open it to take a look) and were the cause of contamination. In hindsight I obviously should've cleaned the room again, I probably should've put the para film on the plates before I turned the hood off as well.

Perhaps I prepared my MEA incorrectly, I am unsure as I followed the directions for the premade agar. It was PCd at 15psi for 30mins. Also condensation was heavy both on the surface of the agar and on the dish itself.

Any tips or comments are appreciated, hopefully I can clean up my act :rolleyes:


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OfflineQuadman
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Steward]
    #23766683 - 10/24/16 12:34 PM (7 years, 3 months ago)

I usually wait and see if anything grows before use.Waiting helps with condensation too. When I pour I try to pour as cool as possible,helps with condensation.


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OfflineSteward
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Quadman]
    #23766909 - 10/24/16 01:55 PM (7 years, 3 months ago)

I agree waiting would've been a good idea, but I decided to try my hand for the first time anyway.

Thank you for your tips, friend. I will pour the next round and wait a couple days, RR mentions leaving plates in their plastic sleeve for 2-3 days to help with condensation. I would try to pour my agar at a cooler temp but I find it solidifying before I can finish.

Hopefully next round wont contain a dust explosion via paper towel in blower intake.


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InvisibleMarty Mycfly
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Steward]
    #23766961 - 10/24/16 02:13 PM (7 years, 3 months ago)

Quote:

Steward said:
Flush #2 is underway, despite some contams on the blocks. Will snag some pictures soon.

What really concerns me is how terrible my agar work is. Despite my flow hood, clean room and "sterile technique" I managed to contaminate an entire sleeve of Petri dish, 4 of them had my ElmA master culture transferred to them. All of them (after a week) showed whitish, round satellite colonies.
The room was sprayed with bleach and left closed with the hood running. Every tool and surface were wiped down with 70% alcohol and, of course, the scalpel was flame sterilized. After I got back in and began wiping my hands, I nearly fell and got a piece of paper towel right into my blowers intake. I'm hoping and praying that remains from the paper towel wont obstruct my hepa( I may open it to take a look) and were the cause of contamination. In hindsight I obviously should've cleaned the room again, I probably should've put the para film on the plates before I turned the hood off as well.

Perhaps I prepared my MEA incorrectly, I am unsure as I followed the directions for the premade agar. It was PCd at 15psi for 30mins. Also condensation was heavy both on the surface of the agar and on the dish itself.

Any tips or comments are appreciated, hopefully I can clean up my act :rolleyes:




As far as the lab being completely cleaned and sterile, this is an en devour that most of us can't accomplish, as long as your working directly in front of the hood and have decent sterile technique you should be fine.
There are a ton of reasons why your whole slip could have got contaminated,
One that sticks out to me is the scalpel, you mentioned flame sterilizing it, but remember that part of your scalpel that isn't flamed is hanging over your plate while working with it, I personally like to sterilize my scalpel in the PC while I am sterilizing the agar, I wrap it in foil and stick it in there in an empty jar or standing up in a test tube rack.

The other thing, as I'm sure your aware of, is being aware of your hand movements, also keeping everything upstream from the dishes. And definitely wrap your dishes in front of the hood with it still on.
Also, don't be discouraged, this is the beginning, you'll be banging out clean myc in no time after spending hours upon hours doing lab work it will become second nature. Your doing awesome!


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InvisibleMycolorado
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Marty Mycfly]
    #23767002 - 10/24/16 02:25 PM (7 years, 3 months ago)

Looking good, Steward!  Like quadman said, pour them cooler and pour them in a stack.  In a stack, only the top plates will get condensation and you can shuffle them to the bottom later to help them clear up.  You can also store them upside down and any condensation will collect on the agar as opposed to the lid.  Not sure what the issue is with your contaminations.  You could let a plate sit uncovered for a minute in the flow and then seal it up and see if anything grows on it...would indicate your filter isn't functioning properly.  Other than that...technique, technique, technique. Keep practicing!


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Edited by Mycolorado (10/24/16 02:25 PM)


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OfflineQuadman
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Mycolorado]
    #23767033 - 10/24/16 02:33 PM (7 years, 3 months ago)

One more tip, I just microwave agar a little to keep pouring if it solidifies. I also microwave after mixing agar and let it solidify before pc. I had a batch show signs of contamination once and immediately sterilized and poured again instead of discarding .


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InvisibleMarty Mycfly
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Quadman]
    #23767074 - 10/24/16 02:49 PM (7 years, 3 months ago)

Quote:

Quadman said:
One more tip, I just microwave agar a little to keep pouring if it solidifies. I also microwave after mixing agar and let it solidify before pc. I had a batch show signs of contamination once and immediately sterilized and poured again instead of discarding .




Nice to hear that, I have seen threads up in mushcult where microwaving agar turns into shit show, in fact a TC (Violet) recently lost her tag over it.


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OfflineQuadman
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Marty Mycfly]
    #23767364 - 10/24/16 04:16 PM (7 years, 3 months ago)

You have to be very careful it boils over quickly, like seconds. Have to replace lost moisture too. How did she loose her tag???


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InvisibleMycolorado
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Quadman]
    #23767431 - 10/24/16 04:36 PM (7 years, 3 months ago)

Quote:

Quadman said:
You have to be very careful it boils over quickly, like seconds. Have to replace lost moisture too. How did she loose her tag???




We always microwaved agar in the mycology lab I worked in.  As quadman said, keep an eye on it so you don't get a mess to clean up.  Other than that, it's a perfectly fine practice.


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InvisibleMarty Mycfly
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Quadman]
    #23767473 - 10/24/16 04:48 PM (7 years, 3 months ago)

Up in that forum anytime someone talks about microwaving it becomes a shit show, She had posted a tek on microwaving and it turned into a brawl until the tek got locked and a mod pulled her tag, she claims she was set up and baited into losing it:shrug: It is such a passionate subject up there, and then you here about a ton of labs like MyColorado worked in that consistently use microwaves.
The Mush Cult forum is a strange place sometimes.


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InvisibleMycolorado
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Marty Mycfly]
    #23767510 - 10/24/16 05:02 PM (7 years, 3 months ago)

Quote:

Marty Mycfly said:
Up in that forum anytime someone talks about microwaving it becomes a shit show, She had posted a tek on microwaving and it turned into a brawl until the tek got locked and a mod pulled her tag, she claims she was set up and baited into losing it:shrug: It is such a passionate subject up there, and then you here about a ton of labs like MyColorado worked in that consistently use microwaves.
The Mush Cult forum is a strange place sometimes.




And just to clarify...I mean microwave to melt if you have a surplus and it's set up...after it's been properly PC'd or autoclaved.

All that shit about losing a TC tag over something like that is a joke.  People need to get a fucking life.  I've posted some things on here based on my experience and people have claimed they're impossible.  If they happen to be a TC or have "clout" on here, all the sheeple will start chiming in, in agreement. Absolutely doesn't make them correct. Kind of funny when all they have to do is start experimenting to find the truth yet they'd rather sling shit.


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stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow


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OfflineSteward
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Mycolorado]
    #23768194 - 10/24/16 08:39 PM (7 years, 3 months ago)

Sweet, thanks for the replies friends~ The devil really is in the detail with mycology, part of what makes it fun.

You may be on to something with the scalpel... I didn't PC mine, just a quick alcohol wipe down. I'll stick it in the PC next round, there's room so it wouldn't hurt.

I think it would be wise to test my flow hood as well. I will keep the microwave in mind should I need it. My handwork and order of operations get screwed up sometimes but you're all right... Practice~

Thanks again, the feedback is so helpful.


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OfflineQuadman
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Re: Tropical Outdoor Fruiting House/Indoor Spawning Chamber [Re: Steward]
    #23768345 - 10/24/16 09:23 PM (7 years, 3 months ago)

Wow that's just crazy.The first time I did agar tried no pour. Thought it was good but my slants were mushy. Then I was reading to cook first. That's when I started using the microwave. Just put in qt jar and heated a few times mixing in between. Let it solidify and put filter lid on and pc. No more problems.i keep extra for later pours and heat a little in microwave.


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