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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Giving Isolating a shot. [Re: X2QE]
#23627257 - 09/08/16 09:16 PM (7 years, 4 months ago) |
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The dog food agar is just really easy and it grows beautiful cultures. It seems to help with making the plates pin too. I had some tampanensis fruit inside the petri dish and I never expected that to happen.
Anyway it can take as little as a week after full colonization. It depends on the mushroom. Cubes usually pin 2 weeks to a month after the plate gets fully colonized. Those invitro pins are also nice for transferring from a contaminated plate.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Giving Isolating a shot. [Re: NumeroEno]
#23627390 - 09/08/16 09:57 PM (7 years, 4 months ago) |
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I have noticed using a gram or two less of the nutrients and pouring thin agar I'll get pins in a couple weeks and it helps with nice rhizo growth
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
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Re: Giving Isolating a shot. [Re: Boogieman47]
#23627487 - 09/08/16 10:33 PM (7 years, 4 months ago) |
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The sooner you can transfer after you see clean growth the better......you aren't SUPPOSED to let a petri colonize fully but I dont always get around to things as fast as I should *sigh*
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 5 hours
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well if your using the plate to noc up masters and your sure your culture is clean then letting it grow almost all the way would be ideal.
oh yeah and dog food agar is awesome got this info from blinding leaf:
"u can use dry dog or cat food plus agar for a recipe. 10g GROUND UP KIBBLE, 10g agar powder, 500ml water. i just use as is straight into PC, but some people simmer it and strain. whatever works for u. seriously a great recipe though."
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Edited by tombosley8 (09/09/16 12:10 AM)
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just_curious
Cultivator


Registered: 03/15/14
Posts: 1,225
Loc: 'Merica (more spcifically...
Last seen: 1 year, 6 months
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Re: Giving Isolating a shot. [Re: mushboy]
#23627875 - 09/09/16 03:14 AM (7 years, 4 months ago) |
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Quote:
just_curious said: For 8 "pasty plates", I use 2.5 grams agar, 2 teaspoons of potato flakes, heat until mostly dissolved. Add in 1.8 grams of honey, and prior to pouring Into plates, put 2 drops of any dark colored food coloring. Pour into your pasty plates and PC at 15psi for 45 minutes,
Quote:
mushboy said: no water?
Lol yes, 1/2 cup of water for the recipe above. Just repeat for 16, 24, etc. Also, one time I only had jell food coloring. This is perfectly fine, just cut back on the honey if you have to use it. Use like 1.3 grams of honey if you use the gel food coloring.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Giving Isolating a shot. [Re: tombosley8]
#23628341 - 09/09/16 08:19 AM (7 years, 4 months ago) |
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Quote:
tombosley8 said: well if your using the plate to noc up masters and your sure your culture is clean then letting it grow almost all the way would be ideal.
oh yeah and dog food agar is awesome got this info from blinding leaf:
"u can use dry dog or cat food plus agar for a recipe. 10g GROUND UP KIBBLE, 10g agar powder, 500ml water. i just use as is straight into PC, but some people simmer it and strain. whatever works for u. seriously a great recipe though."
Yeah blindingleaf turned me onto it too. I feed my dog the nature's domain grain free salmon formula from costco and use that in my agar. When reading the ingredients I noticed that sweet potato is the first ingredient, and the mushrooms seem to love it. Here's a dog food agar plate that I let fully colonize and pin. The pin in the middle was transferred to a new plate. I left this plate on the shelf for a few more days and the pins got pretty big.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
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Re: Giving Isolating a shot. [Re: tombosley8]
#23628373 - 09/09/16 08:37 AM (7 years, 4 months ago) |
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Quote:
tombosley8 said: well if your using the plate to noc up masters and your sure your culture is clean then letting it grow almost all the way would be ideal.
oh yeah and dog food agar is awesome got this info from blinding leaf:
"u can use dry dog or cat food plus agar for a recipe. 10g GROUND UP KIBBLE, 10g agar powder, 500ml water. i just use as is straight into PC, but some people simmer it and strain. whatever works for u. seriously a great recipe though."
How does this smell when you PC it? Id like to try.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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It doesn't smell bad at all. It smells like dog food but it isn't overpowering.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
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Re: Giving Isolating a shot. [Re: NumeroEno]
#23628417 - 09/09/16 08:59 AM (7 years, 4 months ago) |
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Girls don't like pressure cooker smells....
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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The best thing I ever did was buy that 25x. No PC noises or smells in the kitchen. My girlfriend was grateful
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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PsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
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Re: Giving Isolating a shot. [Re: NumeroEno]
#23632438 - 09/10/16 01:35 PM (7 years, 4 months ago) |
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no noise? No smell? Cool, didn't know that about the 25x....doesn't the steam need to escape somehow?
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Wife walks into the kitchen while PC is going.
"That thing stinks."
Me: "Well so do I sometimes, but you don't say anything about it and pretend everything is fine."
Wife smiles in knowing agreement and walks away.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Quote:
PsilocyBen17 said: no noise? No smell? Cool, didn't know that about the 25x....doesn't the steam need to escape somehow?
The only time you let it vent steam is when you're starting your run, and it vents steam quietly. It uses a stopcock instead of a weight, and you control the heat and pressure with a thermostat. Once you start your run it builds pressure, and does an emergency release of the pressure goes over 25. As long as you keep your pressure between 15 and 20 the run is completely silent. When if cools off a vacuum forms so if you want to avoid pulling a vacuum you need to open the stopcock when it's at about 1psi.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 5 hours
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Re: Giving Isolating a shot. [Re: Mushierage]
#23632538 - 09/10/16 02:05 PM (7 years, 4 months ago) |
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yeah dog food is awesome but I think most of the dog food I use for agar is mostly corn, meat/meal, wheat, soybean etc. and since I also use it for mudafuka's bottle tek I went with the one with the lowest fat as he recommended(I think it was him) and the cheapest stuff you can get.
I don't feed my dogs that crap.
They are grain free and salmon eaters too.
Good to know that the good stuff works for agar too.
Could be another good mixup of nutes to keep the cultures from getting lazy.
The cheap stuff is also a good changeup from potato agars since it's mostly corn.
and about the AA electric sterilizers they are very quiet and let off very little steam/smell while cooking, but I always vent the steam immediately after the cycle while still at full pressure so that part is very loud.
I'm just too impatient and busy (and not sure the proper procedure) to come back once it gets down to 1 psi to open the valve before it cools down.
Also when doing muda's bottle tek the dog food does smell quite bad even with the sterilizers so they do let some smell out for sure.
Agar dog food doesn't seem to smell bad probably because it's not being cooked as long.
edit:Quote:
NumeroEno said:
The only time you let it vent steam is when you're starting your run, and it vents steam quietly. It uses a stopcock instead of a weight, and you control the heat and pressure with a thermostat. Once you start your run it builds pressure, and does an emergency release of the pressure goes over 25. As long as you keep your pressure between 15 and 20 the run is completely silent. When if cools off a vacuum forms so if you want to avoid pulling a vacuum you need to open the stopcock when it's at about 1psi.
good to know about the 1 psi on opening the valve.
Edited by tombosley8 (09/10/16 02:11 PM)
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Giving Isolating a shot. [Re: tombosley8]
#23633203 - 09/10/16 05:23 PM (7 years, 4 months ago) |
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I like to let it cool all the way and pull a vacuum. I wrap a clorox wipe around the stopcock and open it in front of the flowhood. With spawn bags it needs upwards of 8 hours to cool anyway, so I usually do my run before bed. I have a countdown timer that I plug it into, so I just let it get up to pressure and set the timer for 4 hours and go to bed. By the time I get home from work the next day the bags are ready to inoculate.
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Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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