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InvisiblexPTxHIPPIe
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Pasty plate question
    #23623608 - 09/07/16 08:36 PM (7 years, 4 months ago)

So with the pasty plates do i need to wrap them with something after inoculation or are they fine just to sit on my shelf as is.

Iv search shroomery for like 3 days and havent found a straight forward answer for this question.


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InvisibleBoogieman47
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Re: Pasty plate question [Re: xPTxHIPPIe]
    #23623614 - 09/07/16 08:38 PM (7 years, 4 months ago)

Well the tek doesn't call for it .... the lids almost snap on so you're good the reason we wrap petris is because they are so loose and is easier to move them around with a safety wrap


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InvisiblexPTxHIPPIe
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Re: Pasty plate question [Re: Boogieman47]
    #23623635 - 09/07/16 08:42 PM (7 years, 4 months ago)

Thats kind of what i was thinking. Its interesting i have a very slow white fuzz growing on most my plates im thinking its myc but its my first time. Im alittle shocked i dont have more contams. Also i had a test plate that i put my bare finger on 2 days ago and nothing grew what so ever so im lost.


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InvisibleBoogieman47
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Re: Pasty plate question [Re: xPTxHIPPIe]
    #23623646 - 09/07/16 08:46 PM (7 years, 4 months ago)

Pics would help if it was an MS syringe it will be tomatose or however you spell it which is a fuzzy growth after a few transfers it will go a bit rhizo if you have lower nutes... I can't remember it's been awhile since I made mine with more nutes if it keeps getting tomatose ?? And as far as you touching it I've seen bacteria show up a week later why did you touch it? Do you have a sab or flow hood ?


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InvisiblexPTxHIPPIe
Inspired
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Registered: 06/13/16
Posts: 273
Loc: Desert
Re: Pasty plate question [Re: Boogieman47]
    #23623655 - 09/07/16 08:51 PM (7 years, 4 months ago)



The touched one isnt even in the picture im working with 20 dishes ms drops 7 with clones and 1 that i touched also have 11 jars would of had 12 but i ran out on the last one pf style.


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InvisiblexPTxHIPPIe
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Registered: 06/13/16
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Re: Pasty plate question [Re: xPTxHIPPIe]
    #23623666 - 09/07/16 08:52 PM (7 years, 4 months ago)

I followed the tek really well but im concerned about the karo i used because it was dark. I bought light karo for my next batch. Also im learned about the pressure build up in my pc i think i have one of those loud ones lol cause i cant get a steady rock on my regulater


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InvisibleBoogieman47
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Re: Pasty plate question [Re: xPTxHIPPIe]
    #23623683 - 09/07/16 08:56 PM (7 years, 4 months ago)

I've never seen people put plates upside down I assume it's for the condensation ? I'd take the paper towels off and put them upright but if it's been working for you stick with it.... what kind of PC? I heard one brand I forget what kind releases pressure every few minutes or so it doesn't rock I have a presto which is pretty easy to keep at 14psi


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InvisiblexPTxHIPPIe
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Re: Pasty plate question [Re: Boogieman47]
    #23623714 - 09/07/16 09:05 PM (7 years, 4 months ago)

I have a mirro 23 qt but yeah the upside down part is for condensation i read that it would help that pic was taken 3 days ago i think on most i have the tiny white fuzz right where the drop went. I have tossed 2 i think because it was an obvious black mold in them of some sort. Btw iv never grown from agar those are my first ever agar plates. I have 2 PF tek grows under my belt and that is all so far.


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InvisiblexPTxHIPPIe
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Re: Pasty plate question [Re: xPTxHIPPIe]
    #23623720 - 09/07/16 09:07 PM (7 years, 4 months ago)

So the paper towels can come off.. As for the upside down part i was concerned about the condensation causing contam issues like iv read about but if i can keep them up right it will be easier to store and make more.


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InvisibleBoogieman47
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Re: Pasty plate question [Re: xPTxHIPPIe]
    #23623739 - 09/07/16 09:10 PM (7 years, 4 months ago)

Ya I think it is the mirro that does the weird release thing ... here is a tomatose that had bacteria but see the fuzzy .. here is a rhizo... as long as you put micro pore tape you'll be good I don't even use the paper towels just foil but it helps with condensation ..


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InvisiblexPTxHIPPIe
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Re: Pasty plate question [Re: Boogieman47]
    #23623763 - 09/07/16 09:15 PM (7 years, 4 months ago)

Yeah my mine look like your first pic just alittle smaller. Id take pics but you wouldnt be able to see anything yet but i plan to keep shroomery posted because well this place is awesome and helped me with my first pf grow which started on my regestration date now im working with agar and boy does it help pass the time between colinization and grows.


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Re: Pasty plate question [Re: xPTxHIPPIe]
    #23623790 - 09/07/16 09:19 PM (7 years, 4 months ago)

Ya that's how all MS start I believe, and this site is pretty badass... and agar is pretty much how I started I tried one syringe inoculation and said Fuck that plus I've only used like .5 cc off my syringes and have had maybe 500 jars ... it can be overwhelming having so many but does keep you busy


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InvisiblexPTxHIPPIe
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Registered: 06/13/16
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Re: Pasty plate question [Re: Boogieman47]
    #23623812 - 09/07/16 09:24 PM (7 years, 4 months ago)

Just curious how do you save the rmainder of your syringe i used the rest of mine in jars because i wasn't sure. Also another wuestion about agar and pcing time. Would it hurt to pc for alittle longer jist to be positive my agar is sterile?


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InvisibleBoogieman47
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Re: Pasty plate question [Re: xPTxHIPPIe]
    #23623840 - 09/07/16 09:29 PM (7 years, 4 months ago)

I have a mini fridge I keep them in the envelope they came in , in the drawer I was worried of how long they would last but bodhi said they keep longer then anything else besides prints ... I PC for 45 minutes I don't think you need more then that


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Re: Pasty plate question [Re: xPTxHIPPIe]
    #23623848 - 09/07/16 09:33 PM (7 years, 4 months ago)

Quote:

xPTxHIPPIe said:
Thats kind of what i was thinking. Its interesting i have a very slow white fuzz growing on most my plates im thinking its myc but its my first time. Im alittle shocked i dont have more contams. Also i had a test plate that i put my bare finger on 2 days ago and nothing grew what so ever so im lost.



I was in the same boat! Good job starting with agar, you won't regret it!!! :laugh:
heres a progression of myc on agar to compare if you like,




1st after xfer:

After a few xfers:


:highfive1: Keep up the good work!


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InvisiblexPTxHIPPIe
Inspired
I'm a teapot


Registered: 06/13/16
Posts: 273
Loc: Desert
Re: Pasty plate question [Re: MysticMoteToter]
    #23623870 - 09/07/16 09:38 PM (7 years, 4 months ago)

Omg
Quote:

MysticMoteToter said:
Quote:

xPTxHIPPIe said:
Thats kind of what i was thinking. Its interesting i have a very slow white fuzz growing on most my plates im thinking its myc but its my first time. Im alittle shocked i dont have more contams. Also i had a test plate that i put my bare finger on 2 days ago and nothing grew what so ever so im lost.



I was in the same boat! Good job starting with agar, you won't regret it!!! :laugh:
heres a progression of myc on agar to compare if you like,




1st after xfer:

After a few xfers:


:highfive1: Keep up the good work!




Omg thank you yeah im day 2 or 3 but im at the second picture on a few and the first on most. Just been concerned about contam but i leave them alone and just let them set on the shelf today was the first day i checked. Btw that order of pictures will be very helpful for alotta noobs on here who are just dipping their feet into agar. Iv looked for a compilation of pictures like that but couldnt find anything


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