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Invisibler.lutece
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Re: Crazy pinning! [Re: just_curious]
    #23626807 - 09/08/16 06:52 PM (7 years, 4 months ago)

Quote:

just_curious said:
I don't need to know anything else lol. Maybe I could post a pic of my jars and you guys could let me know if thats the issue. Pasty, what are the things that you look at on these jars that let know they are ridden with bacteria. Did you know by looking at them, or did you know when you realized that your bulk was going to shit. Is there any key things that you are seeing in those jars?




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InvisiblePastywhyteMDiscord
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Re: Crazy pinning! [Re: r.lutece]
    #23626908 - 09/08/16 07:14 PM (7 years, 4 months ago)

There are a few things to look for. Most people can spot a really bacterial jar by the milky myc or metabolites. But there are some smaller telltale to look for too. Spots where the myc gets really rhizomorphic for no apparent reason, places where the myc appears to shrink onto the grain, slow recovery after a shake. Any of those things and I start to suspect things are not good.

I am really picky about my spawn. If it gives me enough reason to even suspect something then I already am tracing vectors and being paranoid. Some people are like just spawn it anyways. No thanks. I ain't gonna put effort and material into spawning something that's gonna be a mess to clean later. Start being super picky about spawn. Everyone wants to blame the bulk, blame the bucket, blame the grow room. Not me, first thing I suspect is the spawn. Its served me very well.


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Offlinejust_curious
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Re: Crazy pinning! [Re: Pastywhyte]
    #23628650 - 09/09/16 10:40 AM (7 years, 4 months ago)

Well, I'm definitely going to lose this ENTIRE tub. I'm not gonna get a damn thing out of it. Sucks because I needed 100 grams by the end of two weeks....for personal goal reasons of course.


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Offlinejust_curious
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Re: Crazy pinning! [Re: just_curious]
    #23628659 - 09/09/16 10:44 AM (7 years, 4 months ago)

How does this jar look. Given my recent history, I'm assuming bacterial?



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Re: Crazy pinning! [Re: just_curious]
    #23628696 - 09/09/16 10:59 AM (7 years, 4 months ago)

Quote:

just_curious said:
How does this jar look. Given my recent history, I'm assuming bacterial?






It looks a bit weak but more concerning is the slimy grains pressed up against the glass. It's not horrible but if it was mine I would spawn at a higher ratio maybe 1:1.5.

How are you inoculating and expanding the spawn?


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Offlinejust_curious
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Re: Crazy pinning! [Re: Pastywhyte]
    #23628711 - 09/09/16 11:07 AM (7 years, 4 months ago)

I cloned to agar, and then take growth from that to agar one more time (unless bacteria is spotted that is) and then I let the plates grow out and inoculate via "tiger drop." All done within a SAB. I'll inoculate 9 jars, use 7-8 to spawn to CVG, and the other 1-2 to G2G. Never going past 3 transfers. I shower, I wear a face mask, I use alcohol (repeatedly during work to the point that it becomes annoying), I brush my teeth. For my SAB, I lay down paper towel, mix up a spray bottle of anti-bacterial dish soap and water, spray down the paper towels on the bottom, and then spray the shit out of the SAB.

I know that those procedures aren't listed in order...but those are the precautions I take. This is also in a room where there is no free-flowing air because I turn the ceiling fan off, and the air duct is closed up.


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Offlinejust_curious
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Re: Crazy pinning! [Re: just_curious]
    #23628726 - 09/09/16 11:13 AM (7 years, 4 months ago)

I guess I'm confused about what you mean when you say spawn at a higher rate.


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Re: Crazy pinning! [Re: just_curious]
    #23628755 - 09/09/16 11:24 AM (7 years, 4 months ago)

Quote:

just_curious said:
I guess I'm confused about what you mean when you say spawn at a higher rate.




More spawn less bulk. Should colonize stronger and faster which opens your window a bit.


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Offlinejust_curious
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Re: Crazy pinning! [Re: Pastywhyte]
    #23628789 - 09/09/16 11:42 AM (7 years, 4 months ago)

ok, I thought you were talking about the jar. I do 6-7 quarts Spawn to my brick of CVG. But, I usually use so much spawn that i have left over CVG.


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InvisibleliloldmeFacebook
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Re: Crazy pinning! [Re: just_curious]
    #23628854 - 09/09/16 12:06 PM (7 years, 4 months ago)

I'm bad pasty I would have spawned all those jars for a first flush ahaha


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Re: Crazy pinning! [Re: liloldme]
    #23628925 - 09/09/16 12:39 PM (7 years, 4 months ago)

so, you say that it looks weak, but not too much cause for concern and that you would just use more grain for spawning. So, I have 9 of these jars ready to go and I've got 2 weeks to come up with 100-120 grams. Should I spawn them? Also, is there anything that you see that would be causing my trich problem? I could post more and better pics. I really dont want to just throw them out, but if its going to cause another huge mess, then I will chuck them or spawn them outside.


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Re: Crazy pinning! [Re: just_curious]
    #23628931 - 09/09/16 12:42 PM (7 years, 4 months ago)

I would take pasty's advice over mine and toss anything questionable.


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Re: Crazy pinning! [Re: liloldme]
    #23628951 - 09/09/16 12:52 PM (7 years, 4 months ago)

Quote:

liloldme said:
I'm bad pasty I would have spawned all those jars for a first flush ahaha




Well you would have be right to. Only the last set didn't produce a first flush, the others all went for at least 2. But the yield was off. All I am trying to show is that bacteria is sneaky. It can be a mold vector. While many times you will see a few flushes, the yield will often be off.

So if you are seeing a fair amount of mold pre first flush, don't take the spawn for granted. Get picky and tighten up. Use only vigorous cultures, cycle the media, never cut corners on PC cycles. See the results get better.


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Re: Crazy pinning! [Re: Pastywhyte]
    #23628976 - 09/09/16 01:01 PM (7 years, 4 months ago)

I just dont understand how the grain got a bacterial infection because my agar plates were clean...at least appeared to be. No discoloration, no slow growth, etc. Also, why do my jars recover so quickly after a shake given the fact that it is bacteria ridden?


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Re: Crazy pinning! [Re: just_curious]
    #23628996 - 09/09/16 01:07 PM (7 years, 4 months ago)

The bacteria was in the grain. It's nearly impossible to kill all the bacteria in the grain, instead what we are actually doing when we sterilize grain is kill most of it. This lowers the population enough to gain a window to colonize and fruit in.

If the grain had an excessively large population to begin with, a standard 90 min cycle might not be sufficient.

If your grains are recovering fast then it might not be bacteria. Maybe it's just a shitty culture that is not able to withstand the damage done by spawning. Or maybe it's another vector that we are not able to diagnose at the moment.


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Re: Crazy pinning! [Re: Pastywhyte]
    #23629042 - 09/09/16 01:24 PM (7 years, 4 months ago)

And you absolutely don't think it may be a contam in the room or during open air spawning? I've got a mushroom growing in a jar with left over grains and no signs of any molds in it. It is putting off good invitro pins with only the SFD lid on there. Would that be typical of a bacteria contaminated jar?

With the 9 jars that I have, all came from a G2G, is the 2nd transfer, and the master came from clone...what would you honestly do with them? Would you attempt to fruit it, or throw them out.

Also, I've been wondering. Probably a dumb idea, but could you do a soak in anti-bacterial soap-water for say, 5-10 minutes, followed by a nice rinse to get all the soap off. Ive wondered this for both the post-colonized soak, and the 24 hour pre-colonized soak. I'm sure that it's an absolute no-no idea, but I figured it would be worth a shot asking. I know that I soaked my straw with dish soap (pre-spawning) and that didn't seem to hurt the fungi and might've assisted with killing any bacteria. Is it so crazy to think that you couldnt do the same thing with grains.

Maybe I'm not using my PC right. I got one with the weight and have regreted that decision since I bought it.

Also, thanks for all your replies and advice Pasty. I really appreciate the input. I am going to start over, but like I said, I gotta get a little bit more.


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Re: Crazy pinning! [Re: Pastywhyte]
    #23629240 - 09/09/16 02:30 PM (7 years, 4 months ago)

Quote:

Pastywhyte said:
cycle the media





How often should I change up agar recipes?  I didn't want to start a thread for a question like this.


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Re: Crazy pinning! [Re: Kenetic]
    #23629481 - 09/09/16 03:44 PM (7 years, 4 months ago)

I wonder if I should switch up my Agar recipe?


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Re: Crazy pinning! [Re: just_curious]
    #23629509 - 09/09/16 03:52 PM (7 years, 4 months ago)

it's recommended to swap every time you do a transfer at least that what I do. How horrible would it be to create some super isolate that NEEDS potato flakes or it hates life..


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Re: Crazy pinning! [Re: liloldme]
    #23629792 - 09/09/16 05:02 PM (7 years, 4 months ago)

Haaaaahahaha . Dont mind me just enjoying the theories and some dabs.


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