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AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 5 months
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What is a tip that helped reduce your contam rate?
#23622272 - 09/07/16 02:53 PM (7 years, 4 months ago) |
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For me, I started using a still air box with a upside down clear tote and I learned that putting a towel on the bottom and making it wet with soap water helps to reduce shit flying around cause they'll stick to the wet stuff.
Another tip I learned, specifically for agar, was that you're not supposed to put your hand or arm over the agar plate or any open jar.
I started trying to keep my room below 80F as well to not promote bacteria growth as well.
Anyway... what tip can you share with me?
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Morel Guy
Stranger


Registered: 01/23/13
Posts: 15,577
Last seen: 4 years, 1 month
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Re: What is a tip that helped reduce your contam rate? [Re: AK1000]
#23622307 - 09/07/16 03:04 PM (7 years, 4 months ago) |
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Flow hood.
Pretty good idea tho not to put anything over the medium
-------------------- "in sterquiliniis invenitur in stercore invenitur" In filth it will be found in dung it will be found
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Re: What is a tip that helped reduce your contam rate? [Re: Morel Guy]
#23623197 - 09/07/16 06:45 PM (7 years, 4 months ago) |
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-Keeping colonizing substrates above eye level.
-Using a SAB instead of a sealed glovebox.
-Leaving the inside of SAB wet and soapy.
-Learning how to detect contamination so you sure your spawn is clean before spawning.
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
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99.99
Stranger


Registered: 12/22/14
Posts: 1,933
Last seen: 29 minutes, 34 seconds
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Re: What is a tip that helped reduce your contam rate? [Re: Tira]
#23623235 - 09/07/16 06:52 PM (7 years, 4 months ago) |
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Antibiotic agar , lots of colonized agar per bag mixed super super well upon inoculation, after that DONT SHAKE ore mix up containers You should have enough colinized agar in spawn it wont need a mix
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AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 5 months
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Re: What is a tip that helped reduce your contam rate? [Re: 99.99]
#23624041 - 09/07/16 10:19 PM (7 years, 4 months ago) |
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About the antibiotic agar... is that really worth it? Hmm...
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99.99
Stranger


Registered: 12/22/14
Posts: 1,933
Last seen: 29 minutes, 34 seconds
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Re: What is a tip that helped reduce your contam rate? [Re: AK1000]
#23624094 - 09/07/16 10:32 PM (7 years, 4 months ago) |
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Quote:
AK1000 said: About the antibiotic agar... is that really worth it? Hmm...
Bacteria is my problem and the antibiotic agar fixes that
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: What is a tip that helped reduce your contam rate? [Re: 99.99]
#23625184 - 09/08/16 09:23 AM (7 years, 4 months ago) |
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Yeah I'd just do regular agar, and work on your sterile techniques to keep plates from getting bacteria. Only time id use antibacterial agar is if I'm working with a species that fuses with bacteria, like hericium. Or if the spores are extremely bacterial.
Agar is the one and only thing that has almost completely dropped my contam rates.
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Nooner
noon



Registered: 06/15/14
Posts: 95
Last seen: 2 years, 8 months
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Re: What is a tip that helped reduce your contam rate? [Re: Mad Season]
#23625571 - 09/08/16 11:48 AM (7 years, 4 months ago) |
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Do mock up inoculations and agar transfers in your SAB, this way if you see any growth you know it is contamination.
-------------------- Deus Videt
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