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Offlinecityofhomes34
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Registered: 08/14/16
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Last seen: 7 years, 4 months
condensation and liquid in petri plates, melted bottle caps *DELETED*
    #23621462 - 09/07/16 10:28 AM (7 years, 4 months ago)

Post deleted by cityofhomes34

Reason for deletion: figured it out



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Offlinecityofhomes34
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Registered: 08/14/16
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Last seen: 7 years, 4 months
Re: condensation and liquid in petri plates, melted bottle caps [Re: cityofhomes34]
    #23629417 - 09/09/16 03:22 PM (7 years, 4 months ago)

bump


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OfflineKenetic
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Re: condensation and liquid in petri plates, melted bottle caps [Re: cityofhomes34]
    #23629450 - 09/09/16 03:34 PM (7 years, 4 months ago)

They have contaminates.  The blue dots or whatever, plus the bacteria along the edge.  It's pretty obvious actually.  You can still transfer some good myc away from the contams.

But.....  Did you pour then sterilize? What kind of bottle are you using?  And your lid melted because it's a cheap plastic and not useful like polypropylene.  How would aluminum foil keep a cheap lid from melting? 

You don't sprinkle spores on plates. you touch a print and then just touch the center of your dish.  They probably look a lot worse now I bet.


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OfflineMushierage
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Re: condensation and liquid in petri plates, melted bottle caps [Re: Kenetic]
    #23629475 - 09/09/16 03:43 PM (7 years, 4 months ago)

You should have done a little research here before messing with agar bud.  Those plates are contaminated as hell.

Also, incubating at 80 degrees is not helping things.  You are only going to give anaerobic bacteria a jumpstart if any are present.  Once they spread over/around your Myc its going to be tough getting a clean transfer. 

:facepalm:


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Offlinemrmazdarx9
Pffffttt


Registered: 05/15/16
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Re: condensation and liquid in petri plates, melted bottle caps [Re: Mushierage]
    #23629595 - 09/09/16 04:17 PM (7 years, 4 months ago)

They are horrific looks like cottage cheese, did you do anything sterile or just rub a random shroom on agar in a feild


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OfflineMushierage
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Re: condensation and liquid in petri plates, melted bottle caps [Re: Kenetic]
    #23629644 - 09/09/16 04:30 PM (7 years, 4 months ago)

Quote:

kenetic said:
You don't sprinkle spores on plates. you touch a print and then just touch the center of your dish. 




No, OP, not with your finger either.  With a sterile tool, like an inoculation loop or a scalpel.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: condensation and liquid in petri plates, melted bottle caps *DELETED* [Re: cityofhomes34]
    #23629783 - 09/09/16 05:00 PM (7 years, 4 months ago)

.


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Edited by amidogen (04/19/18 09:46 AM)


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OfflineMushierage
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Re: condensation and liquid in petri plates, melted bottle caps *DELETED* [Re: amidogen]
    #23629800 - 09/09/16 05:05 PM (7 years, 4 months ago)

all these people spent their time trying to help you out, and you thank them by deleting your post?

You sir, are an asshat.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineKenetic
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Re: condensation and liquid in petri plates, melted bottle caps [Re: Mushierage]
    #23630124 - 09/09/16 06:27 PM (7 years, 4 months ago)

Quote:

Mushierage said:
Quote:

kenetic said:
You don't sprinkle spores on plates. you touch a print and then just touch the center of your dish. 




No, OP, not with your finger either.  With a sterile tool, like an inoculation loop or a scalpel.




really.  so says the guy pointing the finger at me lol


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineMushierage
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Re: condensation and liquid in petri plates, melted bottle caps [Re: Kenetic]
    #23630269 - 09/09/16 07:07 PM (7 years, 4 months ago)

Too bad everybody couldn't get my humor through text.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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InvisibleInocuole
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Re: condensation and liquid in petri plates, melted bottle caps *DELETED* [Re: cityofhomes34]
    #23630272 - 09/09/16 07:09 PM (7 years, 4 months ago)

Quote:

cityofhomes34 said:
Post deleted by cityofhomes34<p>Reason for deletion: figured it out




:rules:

:dickslap:


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Offlinemrmazdarx9
Pffffttt


Registered: 05/15/16
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Re: condensation and liquid in petri plates, melted bottle caps *DELETED* [Re: Inocuole]
    #23631378 - 09/10/16 05:01 AM (7 years, 4 months ago)

Quote:

Inocuole said:
Quote:

cityofhomes34 said:
Post deleted by cityofhomes34<p>Reason for deletion: figured it out




:rules:

:dickslap:



:rofl:


--------------------
COCA GROWERS come here and share your knowledge
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:penis:Click here for UK trades:penis:
:penis: need some supplies in the UK check Here or PM me:penis:
UK OTD uk members chat
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Right Here
If you use "SWIM" you should DROWN


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