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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Condensation inside Agar PP5
#23620838 - 09/07/16 05:57 AM (7 years, 4 months ago) |
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So I've made some no pour agar PP5's and PC'd em for 40 minutes and left them alone till the PC was cool to touch. Then when I was going to inoculate them I realised that they have a lot of condensation inside of them, and even some droplets on the inside of their lids -you can see it better in the right pic, on the lid of the right pp5). Is this ok? Would it cause any problems? And if it is not ok, what part did I do wrong to cause this?
Thanks in advance .)
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
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dnkronic
Berzerker



Registered: 05/17/08
Posts: 460
Last seen: 11 months, 20 days
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Re: Condensation inside Agar PP5 [Re: Tira]
#23620967 - 09/07/16 07:07 AM (7 years, 4 months ago) |
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Those are cool pp5 plates. Where did u find them? I have the same problem. Pasty himself says to fold a piece of paper towel between the lid and foil to help this. It really hasn't helped for me and was curious if it's possible to put your plates in a container with desiccant to dry them out?? Just an idea DK
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h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
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Re: Condensation inside Agar PP5 [Re: Tira]
#23620976 - 09/07/16 07:13 AM (7 years, 4 months ago) |
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Yes, no and from other posts, when users have allowed the tubs to cool before putting the lids on and brought the temp/pressure up more slowly, the condensation was not as prevalent.
Also, check the pasty plates thread, as many pages as the thread has grown to, there may be some information contained therein that will help rid your tubs of condensation altogether.
Have not heard of desiccant being used, other users have placed a cup of warm water on top for ~20m to eliminate the condensation.
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Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Re: Condensation inside Agar PP5 [Re: h0ldthedoor]
#23620994 - 09/07/16 07:20 AM (7 years, 4 months ago) |
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Quote:
dnkronic said: Those are cool pp5 plates. Where did u find them? I have the same problem. Pasty himself says to fold a piece of paper towel between the lid and foil to help this. It really hasn't helped for me
I just saw them browsing a shop, I live in Turkey and shops here don't have wide variety of products USA has. This was actually the only model I saw so far.
I've read that paper towel trick but didn't try, I'll try it next time.
Quote:
h0ldthedoor said: Yes, no and from other posts, when users have allowed the tubs to cool before putting the lids on and brought the temp/pressure up more slowly, the condensation was not as prevalent.
Also, check the pasty plates thread, as many pages as the thread has grown to, there may be some information contained therein that will help rid your tubs of condensation altogether.
Have not heard of desiccant being used, other users have placed a cup of warm water on top for ~20m to eliminate the condensation.
I did put the lids on after the agar has cooled down and brought the temp of the PC up as slow as possible. I'll check the thread again, I'm sure I'll find something useful.
Thank you both .)
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
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Tookitooki
Mycological Fabricator


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Condensation inside Agar PP5 [Re: Tira]
#23621026 - 09/07/16 07:35 AM (7 years, 4 months ago) |
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Nice looking plates!! I see a lot of colored agar, is this to help get a better visual on growth?
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Condensation inside Agar PP5 [Re: Tookitooki]
#23621042 - 09/07/16 07:40 AM (7 years, 4 months ago) |
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yeson the coloring.
condensation is not a problem. if you dont like having it just leave your plates for a few days and it will disappear.
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Re: Condensation inside Agar PP5 [Re: spacechildo]
#23621059 - 09/07/16 07:46 AM (7 years, 4 months ago) |
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Quote:
Tookitooki said: Nice looking plates!! I see a lot of colored agar, is this to help get a better visual on growth?
easier to spot contaminations and inspect mycelium if agar is dark colored.
Quote:
spacechildo said: yeson the coloring.
condensation is not a problem. if you dont like having it just leave your plates for a few days and it will disappear.
Is it also ok to inoculate before it disappears?
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
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h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
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Re: Condensation inside Agar PP5 [Re: Tira]
#23621075 - 09/07/16 07:53 AM (7 years, 4 months ago) |
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Yes.
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Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Re: Condensation inside Agar PP5 [Re: h0ldthedoor]
#23621113 - 09/07/16 08:08 AM (7 years, 4 months ago) |
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Good to hear .) Thank you all
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: Condensation inside Agar PP5 [Re: Tira]
#23621139 - 09/07/16 08:19 AM (7 years, 4 months ago) |
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I was reading this on boddh.'s signature.
if you pour your dishes correctly at the low temperature you'll end up with something like this
near the top of the stack you might get a few dishes with some condensation, I actually like to pour stacks tall, so I do them 10 high, if you do a bunch of short stacks you get a bunch of dishes with a little bit of condensation if you do a stack of 10 you get 7-8 perfect dishes and 2 you may have to fix.
how do you fix them? use a cup with warm water and a flat bottom, put it on top of the dish for a few seconds-minutes (wont hurt if the water is warm-hot) and boom your dishes are crystal clear again.
Edited by spore-ty (09/07/16 08:22 AM)
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Re: Condensation inside Agar PP5 [Re: spore-ty]
#23621183 - 09/07/16 08:39 AM (7 years, 4 months ago) |
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Quote:
spore-ty said: near the top of the stack you might get a few dishes with some condensation, I actually like to pour stacks tall, so I do them 10 high, if you do a bunch of short stacks you get a bunch of dishes with a little bit of condensation if you do a stack of 10 you get 7-8 perfect dishes and 2 you may have to fix.
That's very useful and good to know, thank you .)
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
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