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cuber3
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First time trays in SGFC, taking a long time?
#23620652 - 09/07/16 03:34 AM (7 years, 4 months ago) |
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Hi all. I'm still a newbie at cultivation. After 3 attempts (first few times I messed up the grain prep) I finally managed to produce a few quarts of spawn, which I spawned around 1:1 to a pasteurized coir/verm/gypsum substrate following Frank's teks.
I used two filter boxes (the ones with the filter strip in the middle, which are also used in growkits), filled them up with the spawn thoroughly mixed in (I realize now it probably would have been better if I had layered it instead of mixing), closed the lid and let them colonize at room temp. I filled a transparent ice cream tub with the rest of the spawn/sub and covered it with aluminum foil. I covered the entire surface of the foil in tiny pinprick holes, which I now realize was overkill and probably detrimental, will not do that again. You can see in the picture that one mangled pin grew prematurely in there, probably because it was getting too much oxygen during colonization..
I filled the filter boxes up too high and the mycelium grew up against the lid, while the bottom took a while longer to colonize. When it was time to fruit, the mycelium had grown flat against the lid, and on one of the two boxes you can see the indentation the filter strip has left.
I placed all three of the trays into my sgfc (holes in all 6 sides, 10cm damp perlite, raised above the ground, humidity seems spot on). I mist/fan 2-4 times a day, but so far about a week has gone by and nothing has happened at all so far. I searched around and see that people are getting hyphal knots and/or pins up to 3 days later, so I'm wondering if I messed things up? I know patience is key, but I just thought I'd post a picture of what I have so I can get some feedback from more experienced growers.
Things I've learned so far: #1: Layer the spawn/substrate instead of trying to mix it up, should hopefully colonize more evenly #2: Don't fill those filter boxes up to the brim anymore
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Tira



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Re: First time trays in SGFC, taking a long time? [Re: cuber3]
#23620672 - 09/07/16 03:50 AM (7 years, 4 months ago) |
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How do your trays smell? Do they have a sourish/sweetish smell?
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
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wtfcrazymofo
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Re: First time trays in SGFC, taking a long time? [Re: cuber3]
#23620679 - 09/07/16 03:57 AM (7 years, 4 months ago) |
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Looks like your gonna see some shroomies. please post future them.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
Edited by wtfcrazymofo (09/07/16 03:59 AM)
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cronicr



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Re: First time trays in SGFC, taking a long time? [Re: cuber3]
#23620717 - 09/07/16 04:39 AM (7 years, 4 months ago) |
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Quote:
cuber3 said:

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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Supalemonhaze
Spore syringe hater.



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Re: First time trays in SGFC, taking a long time? [Re: wtfcrazymofo]
#23620724 - 09/07/16 04:46 AM (7 years, 4 months ago) |
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Quote:
wtfcrazymofo said:
Looks like your gonna see some shroomies. please post future them.

You do realise that those look absolutely horrible.... right?
And OP, don't layer your spawn if you are using coir. Mixing it is better, the only reason why you think that it isn't is because there is a shit-ton of bacteria hindering growth. The only time I would layer spawn and sub is when I'm using straight straw. With straw, the grains will just fall to the bottom when you attempt to mix it, especially if the straw isn't very finely chopped.
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cuber3
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Re: First time trays in SGFC, taking a long time? [Re: Tira]
#23620769 - 09/07/16 05:23 AM (7 years, 4 months ago) |
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Quote:
Tira said: How do your trays smell? Do they have a sourish/sweetish smell?
They smell decidedly mushroomy, similar to how my grain jars smelled when I opened them.
Quote:
Supalemonhaze said:

You do realise that those look absolutely horrible.... right?
And OP, don't layer your spawn if you are using coir. Mixing it is better, the only reason why you think that it isn't is because there is a shit-ton of bacteria hindering growth. The only time I would layer spawn and sub is when I'm using straight straw. With straw, the grains will just fall to the bottom when you attempt to mix it, especially if the straw isn't very finely chopped.
Well, I'm not sure where I went wrong then. My jars were fully colonized and smelled good, I prepped the coir/verm/gypsum substrate according to Frank's tek, checked for proper field capacity and even properly pasteurized it instead of going the bucket way.
I don't know about the ice cream tub, but I think the two filter boxes look so bad because I overfilled them and the myc grew flat against the lid. Could definitely be bacterial though, like you said. Ah well, I'll just keep up the misting/fanning and hopefully I'll at least get something out of it..
Edited by cuber3 (09/07/16 05:43 AM)
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Tira



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Re: First time trays in SGFC, taking a long time? [Re: cuber3]
#23620780 - 09/07/16 05:30 AM (7 years, 4 months ago) |
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Quote:
cuber3 said: They smell decidedly mushroomy, similar to how my grain jars smelled when I opened them.
I asked because they look like they have bacteria or something, that yellowish discoloration is not a sign of healtthy mycelium.
All you can do is to wait and see.
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
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cuber3
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Re: First time trays in SGFC, taking a long time? [Re: Tira]
#23620786 - 09/07/16 05:34 AM (7 years, 4 months ago) |
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Quote:
Tira said:
Quote:
cuber3 said: They smell decidedly mushroomy, similar to how my grain jars smelled when I opened them.
I asked because they look like they have bacteria or something, that yellowish discoloration is not a sign of healtthy mycelium.
All you can do is to wait and see.
Alright, thanks. That sucks to hear  I'll keep up the regular schedule and hope for the best..
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tump
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Re: First time trays in SGFC, taking a long time? [Re: cuber3]
#23620790 - 09/07/16 05:37 AM (7 years, 4 months ago) |
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I hope him well. Mixing the sub randomly with grains works not a whole lot better then layering. Besides one on the right might turn out a few.
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Tira



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Re: First time trays in SGFC, taking a long time? [Re: cuber3]
#23620793 - 09/07/16 05:38 AM (7 years, 4 months ago) |
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Quote:
cuber3 said:
Quote:
Tira said:
Quote:
cuber3 said: They smell decidedly mushroomy, similar to how my grain jars smelled when I opened them.
I asked because they look like they have bacteria or something, that yellowish discoloration is not a sign of healtthy mycelium.
All you can do is to wait and see.
Alright, thanks. That sucks to hear  I'll keep up the regular schedule and hope for the best..
Don't give up hope! I had flushes from worse looking substrates than those.
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
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Supalemonhaze
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Re: First time trays in SGFC, taking a long time? [Re: Tira]
#23621336 - 09/07/16 09:52 AM (7 years, 4 months ago) |
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Whenever you get that mycelium mat on the surface of your sub, it's bacteria. Filling your trays to the brim had nothing to do with it. You don't really have to close the lids to those containers by the way, you can just put them in fruiting conditions from day 1.
I'm sure that your grains had signs of bacteria while in the jars. Do you have any pics of them? IME, I was never able to sniff out a contam in a jar. I'm better off relying on my eyes. Besides, there are countless of bacteria species that don't smell at all and the mycelium can easily overpower any other smells in the jar. Generally, when the mycelium in grain jars grows very thick, it's bacterial. By very thick I mean that the grains are not visible through the mycelium and you are not able to break the colonized grains by hand. Whenever you shake a grain jar and are unable to break all the clumps of mycelium, it's bacterial. There are other signs, I suggest that you post pics of your jars before you spawn them, that way you can learn for yourself what you need to look for.
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Mushierage
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Re: First time trays in SGFC, taking a long time? [Re: Supalemonhaze]
#23621360 - 09/07/16 10:00 AM (7 years, 4 months ago) |
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Pretty bacterial. But as long as you get those into fruiting conditions, you still might be able to pull off a couple of decent flushes from them.
Good luck on fruiting, and in the meantime try to work out why your spawn is getting dirty.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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NwMycoHead
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Re: First time trays in SGFC, taking a long time? [Re: Mushierage]
#23621486 - 09/07/16 10:36 AM (7 years, 4 months ago) |
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Love reading through these feeds, you learn so much everyday. I've also heard myc will have a slight yellow tint if it's not hydrated properly could this be the case here or no definitely bacterial? And what's a good way to tell the difference to an untrained eye?
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Mushierage
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Re: First time trays in SGFC, taking a long time? [Re: NwMycoHead]
#23621493 - 09/07/16 10:39 AM (7 years, 4 months ago) |
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Myc that isn't properly hydrated will look dry. If it's really dry, it might start bruising too.
Mycelial metabolites (the yellowing) is generally a reaction to a foreign substance the Myc comes up against, or a result of the Myc being stressed. Also, you can tell its bacterial, because it looks puffy, matted, and like melting ice cream.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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Supalemonhaze
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Re: First time trays in SGFC, taking a long time? [Re: NwMycoHead]
#23621512 - 09/07/16 10:44 AM (7 years, 4 months ago) |
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Nope, definitely metabolites. That mycelial mat is the all telling sign. What you are talking about is still normally caused by bacteria but aggravated by lack of misting. It will look more like scorched skin than anything else.
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cuber3
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Re: First time trays in SGFC, taking a long time? [Re: Supalemonhaze]
#23621703 - 09/07/16 11:37 AM (7 years, 4 months ago) |
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Alright, thanks for the replies and explanations everyone, definitely helps to know the telltale signs of bacteria. I've only dealt with trich and/or penicillin before afaik. I will definitely try to up my sterile technique in the future. I'm a bit confused since the spawn looked pure white and smelled good, and I always heard coir was very contam-resistant and practically foolproof for a beginner. I spawned in the open and didn't bleach the area (did thoroughly clean beforehand though), maybe that's where things went wrong. Next time I'll be doing this in a well-bleached SAB. I'm starting a fresh round of grains soon, this time using proper self healing injection port lids and some LC (which will also be a first for me). Hopefully I can report back with some healthier looking mycelium then, and not make the same mistakes again 
Quote:
Supalemonhaze said: You don't really have to close the lids to those containers by the way, you can just put them in fruiting conditions from day 1.
Sorry, I don't fully understand what you mean by this. I need to fully colonize the trays first (with the lid on for high co2) before moving them into the fruiting chamber, right?
Quote:
Supalemonhaze said: I'm sure that your grains had signs of bacteria while in the jars. Do you have any pics of them? IME, I was never able to sniff out a contam in a jar. I'm better off relying on my eyes. Besides, there are countless of bacteria species that don't smell at all and the mycelium can easily overpower any other smells in the jar. Generally, when the mycelium in grain jars grows very thick, it's bacterial. By very thick I mean that the grains are not visible through the mycelium and you are not able to break the colonized grains by hand. Whenever you shake a grain jar and are unable to break all the clumps of mycelium, it's bacterial. There are other signs, I suggest that you post pics of your jars before you spawn them, that way you can learn for yourself what you need to look for.
Thanks for the info, I didn't know about any of this. I guess this might have been the case then, I'm not sure. I definitely couldn't see the majority of the grains anymore, and I had to bang it quite hard to shake the grains up. The grains separated quite well, but there were still some clumps left that I couldn't bang loose and had to break up with my hands. Here are a few pics of the jars that I took a while ago, shortly before I spawned them, one is WBS, the other rye:



The rye one had some thick cottony growth at the top:

I do have to add that my inoculation procedure wasn't entirely up to snuff. I used synthetic filter discs on my jars, with the metal lid underneath with 4 small holes punched in them for GE. I realized I couldn't inoculate through the filter disc, so I ended up quickly opening/closing the lids in my SAB to inoculate them (MS syringe), which could surely have invited some contams. Two of my jars didn't make it because of trich. I recently built proper self healing injection port lids so I don't have to do that anymore next time around..
Edited by cuber3 (09/07/16 02:51 PM)
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NwMycoHead
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Re: First time trays in SGFC, taking a long time? [Re: Supalemonhaze]
#23621994 - 09/07/16 01:16 PM (7 years, 4 months ago) |
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Quote:
Mushierage said: Myc that isn't properly hydrated will look dry. If it's really dry, it might start bruising too.
Mycelial metabolites (the yellowing) is generally a reaction to a foreign substance the Myc comes up against, or a result of the Myc being stressed. Also, you can tell its bacterial, because it looks puffy, matted, and like melting ice cream.
Quote:
Supalemonhaze said: Nope, definitely metabolites. That mycelial mat is the all telling sign. What you are talking about is still normally caused by bacteria but aggravated by lack of misting. It will look more like scorched skin than anything else.
Thanks, very good to know.
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cuber3
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Re: First time trays in SGFC, taking a long time? [Re: NwMycoHead]
#23628112 - 09/09/16 05:55 AM (7 years, 4 months ago) |
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Ok, so I had 4 more trays colonizing prepped in the same way, and every single one ended up contaminated. 3 out of 4 got trich and the fourth which seemed to have some nice rhizo growth and was looking healthy from the outside had its surface covered in bright orange and webby mold.
None of the three trays have shown any growth for the 2 weeks they have been in my fruiting chamber so far, so I'm starting to think this is a lost cause. Can anyone see anything wrong with the pics of the colonized grains I posted above?
I'm going to start a bunch of PF jars soon to tide me over in the meantime, and then I'd like to try another round of grains, hopefully with better luck. I'm actually hoping that the problem lies with my inoculation technique, and that my new self healing injection port lids might resolve the issue.. I still think it's weird that I ended up with so much trich in the coir trays, while the spawn looked clean and coir is supposedly so contam-resistant.
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wtfcrazymofo
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Re: First time trays in SGFC, taking a long time? [Re: cuber3]
#23631373 - 09/10/16 04:49 AM (7 years, 4 months ago) |
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Keep on practicing. You should use agar first before you go to grain.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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tump
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Re: First time trays in SGFC, taking a long time? [Re: wtfcrazymofo]
#23631446 - 09/10/16 06:40 AM (7 years, 4 months ago) |
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No offence to everyone who uses agar. But agar isn't fool poof. Sometimes you get unlucky with trich. But many noobs have some luck on grains with spores to grains With out as agar work. True that nothing beats speed as a blended li. But nothing is easier to mess up too. You leave the lid off a jar to long. Its contams. You leave the lid off the li to long it all contaminate to hell. With agar work you need good handy skills. Same with agar to grain then grain to grain. Every contact if a bleed for more contams. Long long time ago when i started with bird seed and spores syringes i had 95% succes rate. With agar its like 70% succes rate.
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wtfcrazymofo
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Re: First time trays in SGFC, taking a long time? [Re: tump]
#23634803 - 09/11/16 06:41 AM (7 years, 4 months ago) |
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Nothing is fool proof even fools mess up brf cakes.
Agar takes longer, and requires more logical sterile skill that fools can take some time to learn.
I would like to give a shot out to agar!
Agar rules. All the contams reveal themselves, and all you got to do is transfer away from those nasteys, or just throw them in the compost.
Once you got a clean agar plate it is still not fool proof. If you made a clean agar plate you might not be a fool. but Shooting syringe juice may be fooley unless you got some clean spore juice.
If you got deliberate swift motions of the hands (not above the plate) and sterile skills agar is tits.
Did OP use agar? or was he fa-fa-fa foolen?
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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cuber3
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Re: First time trays in SGFC, taking a long time? [Re: wtfcrazymofo]
#23636432 - 09/11/16 05:06 PM (7 years, 4 months ago) |
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Quote:
wtfcrazymofo said: Keep on practicing. You should use agar first before you go to grain.
Quote:
wtfcrazymofo said:Did OP use agar? or was he fa-fa-fa foolen?
I did not use agar for this grow, just a plain MS syringe to simplify things for my first real grow. I totally get where you're coming from though, isolating healthy myc and then also having it colonize the grains much quicker than with spores is obviously a superior way to go. I have read up on agar, and have all the necessary supplies so I will probably try my hand at it very soon. The problem is that I have had some contamination issues using my glovebox (granted, I didn't clean it out with bleach but rather with isopropyl alcohol, which I've read isn't enough. I did use a spray bottle to mist the air inside the glovebox with iso and wiped all the sides with it, wear alcohol sanitized gloves, flame sterilized needle etc). Still a few of my spawn jars ended up with trich (ocracking open a corner of the lids, not using injection ports which I will next time around). I'm a bit afraid that I won't be able to pull agar and/or g2g off without a proper flow hood this way, maybe switching from iso to bleach could solve my issues? Still, will give it a shot soon, if at least to get some practice and experience with it, regardless of the outcome. First time for everything right, all at its own pace :-)
This morning I was pleasantly surprised to see that the ice cream tub (the one on the right in the OP) is now showing several dozen pins, I had pretty much given up on it since they looked so bad and so much time had passed already. The tray on the left in the OP is also showing a couple pins, though much much less than the other one. The middle tray which looked the worst is still showing nothing at all. Maybe I'll still get a somewhat decent harvest off that one tray after all, I'll report back later hopefully with some pics of mature fruits..
Edited by cuber3 (09/11/16 05:27 PM)
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wtfcrazymofo
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Re: First time trays in SGFC, taking a long time? [Re: cuber3]
#23642396 - 09/13/16 05:48 PM (7 years, 4 months ago) |
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You dont need a flowhood but they do rule for 100 jars a week or so. Dont forget to get the walls of your sab wet with soapy water after treatment with iso. Some say you can skip the iso and you only got to get the inside wet so nothing falls off of the inside of the box. and wait 10-15-20 minutes for the air to settle inside the box. and It can't be in a drafty area whatsoever.
Work in the back of the box with 2 holes. Don't go over the media with your hands.
It might of been your syringe or wet grain not your sab. You dont need ships. bleech isn't typically used for sab work.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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cuber3
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Re: First time trays in SGFC, taking a long time? [Re: wtfcrazymofo]
#23643832 - 09/14/16 05:11 AM (7 years, 4 months ago) |
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Quote:
wtfcrazymofo said: You dont need a flowhood but they do rule for 100 jars a week or so. Dont forget to get the walls of your sab wet with soapy water after treatment with iso. Some say you can skip the iso and you only got to get the inside wet so nothing falls off of the inside of the box. and wait 10-15-20 minutes for the air to settle inside the box. and It can't be in a drafty area whatsoever.
Work in the back of the box with 2 holes. Don't go over the media with your hands.
It might of been your syringe or wet grain not your sab. You dont need ships. bleech isn't typically used for sab work.
Alright, thanks for the info, I'll take all of that into account next time I knock up some jars. I think I might not have waited long enough for the air to settle inside the box and that could be my problem. Popping open the lids might have sucked in some contaminants that hadn't settled yet.
Some good news at least, today I harvested my first flush from the healthiest looking tub:




Haven't weighed them yet as I didn't want to handle 'em too much while still wet. Still, I'm pretty happy with the results. They're in my dehydrator at 105F right now, curious what the dry weight will be. The block of myc is rehydrating right now, hopefully I can get a few more flushes out of it. If only my other tubs didn't get contaminated along the way :-)
The first filter box on the left in my OP had developed pins a couple days ago, and they've grown somewhat in size, though today it isn't looking too good at all:


Not sure if there's anything I can do about it, it has been getting the same misting/fanning treatment as the other one. What does the premature opening/curling of the caps say? I read that premature openign of caps could be due to overwatering, though I've also seen pics of similar looking ones and it was said that it was likely just a mutation and that they could still grow to full size. I guess we'll have to wait and see.. They're growing much slower than the other tub in any case, which seems to not be a good sign..
The middle one in my OP I think is a lost cause. It's the worst looking of the three and though it has now also developed a couple pinheads, I don't think they have any chance of maturing.
Edited by cuber3 (09/14/16 05:20 AM)
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PortabellaFella 1
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Re: First time trays in SGFC, taking a long time? [Re: cuber3]
#23643915 - 09/14/16 06:31 AM (7 years, 4 months ago) |
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The curled open ones are mutations.
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cuber3
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Quote:
PortabellaFella 1 said: The curled open ones are mutations.
Okay, thanks! I'm hoping they'll put on some more size soon, we'll just have to wait and see.
I'm still confused by this post:
Quote:
Supalemonhaze said: You don't really have to close the lids to those containers by the way, you can just put them in fruiting conditions from day 1.
This seems to imply that I could throw a freshly spawned tray into my fruiting chamber without first letting it fully colonize with the lid closed and a few holes for gas exchange. I know that with a monotub you can colonize inside the tub by plugging up some of the holes to prevent air exchange, but I'm using non-cased coir/verm/gypsum in a SGFC. Also, he is saying "fruiting conditions", so air exchange + misting/fanning. Won't the sub then simply refuse to colonize, or will it still colonize and find its way to the surface to fruit as soon as possible? I always read you want 100% colonization before introducing to fruiting conditions, that once fruiting conditions are introduced the myc will focus on fruiting and stop colonizing, and any uncolonized sub at that point is just lost nutrients and a possible vector for contamination. Can anyone elaborate on this please?
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Supalemonhaze
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Re: First time trays in SGFC, taking a long time? [Re: cuber3]
#23644194 - 09/14/16 09:40 AM (7 years, 4 months ago) |
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cuber3 said:
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PortabellaFella 1 said: The curled open ones are mutations.
Okay, thanks! I'm hoping they'll put on some more size soon, we'll just have to wait and see.
I'm still confused by this post:
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Supalemonhaze said: You don't really have to close the lids to those containers by the way, you can just put them in fruiting conditions from day 1.
This seems to imply that I could throw a freshly spawned tray into my fruiting chamber without first letting it fully colonize with the lid closed and a few holes for gas exchange. I know that with a monotub you can colonize inside the tub by plugging up some of the holes to prevent air exchange, but I'm using non-cased coir/verm/gypsum in a SGFC. Also, he is saying "fruiting conditions", so air exchange + misting/fanning. Won't the sub then simply refuse to colonize, or will it still colonize and find its way to the surface to fruit as soon as possible? I always read you want 100% colonization before introducing to fruiting conditions, that once fruiting conditions are introduced the myc will focus on fruiting and stop colonizing, and any uncolonized sub at that point is just lost nutrients and a possible vector for contamination. Can anyone elaborate on this please?
What I mean by fruiting at spawning is exactly how it reads. You take your trays, fill a monotub with them and stuff in your poly like you would do on any other grow.
When you really think about it, mycelium will fruit whenever it damn well pleases. Mycelium pins on agar with minimal GE (no FAE whatsoever), as well as in grain jars. However, FAE will not stop mycelium from colonizing. Actually, fruiting at spawning will shave off a few days from spawn to harvest. It's not that colonization is faster, it's that the mycelium senses that it's in a good enough enviroment to fruit in while it's still colonizing so as soon as it consolidates, it fruits.
Here's Eat's thread about casing and fruiting at spawning, he also shows a side by side experiment using both methods to show earlier pinning on the ones that were fruited and cased at spawning.
https://www.shroomery.org/forums/showflat.php/Number/22315950/fpart/1/vc/1/nt/27
If I had to guess, I would say that colonizing with high CO2 was simply an effort to replicate nature originally. Mycology is still a rather new science and it was hard to pull off a grow inside in the past so replicating nature was an obvious way to start. Nowadays, we know that a substrate that was fruited right after it was spawned will pin faster than the regular method but it will lose more moisture due to the earlier exposure to FAE. I only mention this because you are using trays, if your trays are thin, you may notice that you need to mist before the 1st flush finishes vs not misting at all on 1st flush with thicker subs.
So yeah, definitely not something you need to worry about, the mycelium won't mind either way. It's just a matter of what you prefer to do.
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StickyIcky Fingers
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Re: First time trays in SGFC, taking a long time? [Re: cuber3]
#23644235 - 09/14/16 09:57 AM (7 years, 4 months ago) |
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cuber3 said:
The problem is that I have had some contamination issues using my glovebox (granted, I didn't clean it out with bleach but rather with isopropyl alcohol, which I've read isn't enough.
It could be your syringe or your sterile technique sometimes I will leave an agar dish (that I'm not going to noc up) sitting with the lid off as I work in my SAB to see if it contams.... to check the SAB.
Also many posts ago you said you might make up an LC, I strongly suggest holding off on this until you can get/keep stuff clean an LC is only going to exacerbate your contam issues. If you later decide to do an LC noc it up with agar and check the LC on agar.
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Mad Season
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Actually many years ago people skipped colonization and went straight to fruiting like azur has. They concluded tho that it was taking away needed energy for fruiting.
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cronicr said: i don't know what your train of thought is on taping holes and co2/fae during colonizing but here's my thought.. We only tape the holes to up the co2 levels, this does a couple things, it slows down how fast the substrate is consumed while colonizing and the reduction of co2 at fruiting is a pinning trigger.
We don't want the substrate to be devoured during colonizing we only want it colonized , we want it devoured once put in to fruiting conditons and this allows much fuller flushes, this is why you only see subs pull away from the edges of the tubs after fruiting because that is when it is really eating it. We keep a high CO2 level during colonization because the myceliums job in nature is to break down solid matter, releasing its carbon as CO2. in open air, most of the substrate will have been consumed by the time fruiting happens.
Also this means that while your giving your substrate fae you should also see the sub getting the fresh air exchange colonize faster then the one that doesn't and has a higher co2 level, but for the reasons i just stated this is not a good thing.
That being said, monos have such a huge substrate, it really doesn't matter if some of the potential gets used, because we generally only want a couple big flushes anyways before tossing.
If this was a more nutritious substrate with less water (pf cakes) colonizing in fruiting would be very detrimental. I actually don't even spawn at fruiting with small shoe box trays.
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wtfcrazymofo
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Re: First time trays in SGFC, taking a long time? [Re: cuber3]
#23645193 - 09/14/16 04:43 PM (7 years, 4 months ago) |
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Nice shroomies eat fresheys.
Clone a good one to agar with just a tiny rice sized piece from the inside of the stem. If those were bacterial would it carry on the bacteria?
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Supalemonhaze
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Re: First time trays in SGFC, taking a long time? [Re: wtfcrazymofo]
#23646606 - 09/15/16 05:18 AM (7 years, 4 months ago) |
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Yes, it would you have to clean it up on your next transfer. Bacteria can be really sneaky too, sometimes it will be totally invisible. Do an agar sandwich to clean bacterial cultures.
I suggest taking multiple biopsies from the same mushroom, bacteria can completely halt growth when it's bad. I have had clone tissue that never recovered on agar, you can't clean up a culture that refuses to grow.
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twistedty
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Re: First time trays in SGFC, taking a long time? [Re: Supalemonhaze]
#23646611 - 09/15/16 05:21 AM (7 years, 4 months ago) |
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Sexy fruits man. Love it
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cuber3
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Re: First time trays in SGFC, taking a long time? [Re: twistedty]
#23661630 - 09/20/16 12:39 PM (7 years, 4 months ago) |
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Another quick update. In the end, all three trays fruited pretty well, though the most healthy one turned out the best. The other two trays grew a lot of mutants, where most of the caps curled upwards, not sure if it is just a coincidence or if the bacterial infection had anything to do with that.. Either way, all trays grew plenty of pins which quickly matured into short but meaty mushrooms.
First flushes of three trays combined yielded a good 24 grams cracker dry. Not sure how good of a yield that is for this size trays, but I'm pretty happy how it turned out considering the bacterial contamination, hopefully they will turn out some more shroomies on the next flush. In the meantime I'm already thinking about starting some new grain, and hopefully the next grow will turn out better with some healthier looking trays to start out with. Thanks again for the tips and suggestions everyone, I learned a lot from this and am sure this was a good step in improving my future technique
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Tira



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Re: First time trays in SGFC, taking a long time? [Re: cuber3]
#23662465 - 09/20/16 05:34 PM (7 years, 4 months ago) |
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Those flushes look good man .) congratz
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
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