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InvisibleBunya
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oyster mycelium on agar to dowle.
    #23620599 - 09/07/16 02:29 AM (7 years, 4 months ago)

Whats the best method.
I have some good clean mycelium growing on agar and want to put to dowles, to inoculate some fresh cut logs.
Am i best making an LC from the agar and inoculate the dowles in a grow bag? Or just put an agar wedge in the grow bag with the dowle?
Should i add grain(wbs) in the grow bag with the dowle.
Im thinking of buying the dowel(is there a preferred diameter?)
And cutting rings in it with a dremel. Is there a prefered length of dowle?
Any help or ideas would be greatly appreciated.
Pic of mycelium on https://files.shroomery.org/files/16-36/322753908-20160907_153826.jpg


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Edited by Bunya (09/07/16 02:30 AM)


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OfflineFerather
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Re: oyster mycelium on agar to dowle. [Re: Bunya] * 1
    #23621312 - 09/07/16 09:44 AM (7 years, 4 months ago)

If you have a lot of dowels, like you said, no different to inoculating a substrate.
A bag of dowels is the same as a bag of sawdust for your purpose.

My vendor uses 8mm x 35mm dowels, spiral groove.
30 plugs per 50 cm length of timber.

8.5mm drill bit for holes.


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OfflineTravelAgency
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Re: oyster mycelium on agar to dowle. [Re: Ferather]
    #23621843 - 09/07/16 12:23 PM (7 years, 4 months ago)

I'd love to see this process.

:threadmonitor:


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InvisibleBunya
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Re: oyster mycelium on agar to dowle. [Re: TravelAgency]
    #23622064 - 09/07/16 01:42 PM (7 years, 4 months ago)

Thanks Ferather.
Those dimensions are very helpful.
TravelAgency,I will try to update.


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OfflineFerather
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Re: oyster mycelium on agar to dowle. [Re: Bunya] * 1
    #23622263 - 09/07/16 02:51 PM (7 years, 4 months ago)

*Optional*

You can add some plant food to your water to improve the growth.
Any is good, but I find certain brands are more rich.

Just use the stated dose on the label.
Alternatively read the WL Tek.

Better than just dowels.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineQuadman
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Re: oyster mycelium on agar to dowle. [Re: Ferather] * 1
    #23622517 - 09/07/16 04:03 PM (7 years, 4 months ago)

I would go agar> grain> dowels. Wood working dowels have grooves or spirals already in them. Sterilize the dowels after soaking. Agar to dowels would have  a lot of air spaces.


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OfflineFerather
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Re: oyster mycelium on agar to dowle. [Re: Quadman]
    #23622707 - 09/07/16 04:54 PM (7 years, 4 months ago)

True, you would also get faster times with spawn to dowels.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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InvisibleBunya
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Re: oyster mycelium on agar to dowle. [Re: Ferather]
    #23638722 - 09/12/16 03:04 PM (7 years, 4 months ago)

Thanks for your help guys
Ive ordered some dowles and waiting for my grain and LC to colonize.
Wanting to get things started.
I made some dowles,cooked them and sterilzed them with some finely chopped straw and inoculated with an agar wedge.

The dowles wouldnt sink with the cooking hoping they took on enough water.


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Offlinekatbusa
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Re: oyster mycelium on agar to dowle. [Re: Bunya] * 1
    #23655395 - 09/18/16 11:30 AM (7 years, 4 months ago)

They will sink. You gotta give them time.


When i make plugs I gently boil them till they are soo water logged that they sink. Can take 45 min sometimes. Then into bags they go.

Here is a batch i made last night. 4,000 plugs that will be put into logs that will be incubating in a green house over the winter.



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InvisibleBunya
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Re: oyster mycelium on agar to dowle. [Re: katbusa]
    #23657459 - 09/19/16 12:20 AM (7 years, 4 months ago)

Quote:

katbusa said:
They will sink. You gotta give them time.


When i make plugs I gently boil them till they are soo water logged that they sink. Can take 45 min sometimes. Then into bags they go.

Here is a batch i made last night. 4,000 plugs that will be put into logs that will be incubating in a green house over the winter.





Excelent would love to see pics of your work.
Ive got some soaking in sealed jars to waterlog them. Last lot simmered for over an hour. When the LC is ready a few more days i was going to throw some soaked wbs in the bag with the plugs. Sterilize and inoculate. Wait a week then chop a tree down. And plug it up fresh. Does that sound like a good plan?
How long does the average log take to colinize? 6mths?


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Offlinekatbusa
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Re: oyster mycelium on agar to dowle. [Re: Bunya] * 1
    #23658330 - 09/19/16 10:08 AM (7 years, 4 months ago)

I would be more than glad to share some info and pictures as I go. I just got back into mycology after almost 2 years break. Got a new job to requires me to travel all over the USA but things are calming down and I now will be spending only shot spurts away from home.

Thankfully I kept spores from the isolates I made a few years back so I can start up again.

Your plan for the plugs sounds good. The only thing I would change is to cut down the tree of limbs two weeks before you want to inoculate. This will give the logs some time to die off and age a little.

Also the best time to cut logs and tree is when the leaves start to change and in the spring due to the sap and nutrients in the tree are mobile and will be concentrated in the wood. You still can cut wood any part of the year and still get good results.

When it comes to the logs being colonized and starting to fruit. It all depends on the weather and temps. I like to inoculate logs in early fall and don't usually expect any mushrooms till 8-12 months later.

Good luck and happy growing!


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Offlineadadada
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Re: oyster mycelium on agar to dowle. [Re: Bunya] * 1
    #23658459 - 09/19/16 11:10 AM (7 years, 4 months ago)

You can inoculate a tree immediately after felling but I've been told that you'll get better results if you wait 2 weeks.  The theory is that this allows anti-fungal compounds within the tree to degrade.

Also, if we're talking about deciduous trees (e.g. oak) then you'd probably get better results by felling the tree only after it's dropped its leaves and gone dormant.  I've been told that there are more nutrients stored in the trunk and branches while the tree is dormant than while it's actively growing.

Caveat emptor:  I haven't tested either of these claims but do follow them and have had decent results.


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Offlineadadada
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Re: oyster mycelium on agar to dowle. [Re: adadada]
    #23658479 - 09/19/16 11:16 AM (7 years, 4 months ago)

Btw, I agree with going agar -> grain -> dowel.

I've tried going straight from agar -> dowel and the growth of the mycelium was very slow.  It did eventually colonize but only after a few months.  I was very fortunate that it didn't contaminate.

The mycelium seems to need the extra vigor from the grain to colonize the low nutrient dowels quickly.

Also, if you're going to use grain, I'd sterilize the dowels and do the transfer in a sterile environment ... not open air.  I'd be worried about contamination (trichoderma) getting on the grain spawn and getting a foothold.  Maybe not a big concern if the grain is fully colonized but you never can be too careful.

Good thing about dowels though ... you can always dry them out and try again. :smile:

adadada


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InvisibleBunya
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Re: oyster mycelium on agar to dowle. [Re: adadada]
    #23663962 - 09/21/16 01:40 AM (7 years, 4 months ago)

Thanks for you help and advice guys really appreciate the tips.
On you advice i cut a tree down today.(local weed tree)
Its quite hard wood, burns well in the fire usually
I could only carry lenths 1.2m long and are about 200mm wide.
I will probably cut them to 600mm long to be more manageable, and then paint the ends with a waterbase bitumen tanking paint i have that is safe to use in drinking water tanks.

Should i keep it dry untill i plug it?
Will 300mm lenghts work well aswell?


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OfflineQuadman
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Re: oyster mycelium on agar to dowle. *DELETED* [Re: Bunya]
    #23664149 - 09/21/16 05:33 AM (7 years, 4 months ago)

Post deleted by Quadman

Reason for deletion: Wrong info



Edited by Quadman (09/21/16 05:40 AM)


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OfflineQuadman
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Re: oyster mycelium on agar to dowle. [Re: Quadman]
    #23664330 - 09/21/16 08:05 AM (7 years, 4 months ago)

I was wrong Stamets recommends waxing the end of the log after inserting the plugs. Water is absorbed through the bark.


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OfflineQuadman
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Re: oyster mycelium on agar to dowle. [Re: Quadman] * 1
    #23664386 - 09/21/16 08:37 AM (7 years, 4 months ago)

Logs from healthy trees, bark intact.
Size 3-4 feet long  4-8 inches in diameter
Keep logs off ground to keep clean for at least 2 weeks.pallets work good
Store no longer than 6 months uninoculated
Below 40 degreesF must be kept warm for at least 30 days
Holes no more than 4 inches apart
Wax holes after plugging and wax both ends
During incubation place off ground ,stacked criss cross.
Locate in moist shady area ,tree shade, shade cloth, etc
Water once or twice per week for 5-10 minutes
Do not cover with plastic
Cover with burlap or cloth support several inches above logs moist cloth will help keep moisture.

Information from Fungi Perfecti


Edited by Quadman (09/21/16 08:39 AM)


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InvisibleBunya
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Re: oyster mycelium on agar to dowle. [Re: Quadman]
    #23664413 - 09/21/16 08:47 AM (7 years, 4 months ago)

Thanks Quadman.
Excelent info.
Coming into spring here so think i might have the timing right.
Have just the spot for them. Used to stack firewood there but it never really dried and often rotted.
I have an old pallet to stack them on off the ground.


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OfflineQuadman
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Re: oyster mycelium on agar to dowle. [Re: Bunya]
    #23664488 - 09/21/16 09:28 AM (7 years, 4 months ago)

Should be good then. They recommend cutting just before leaves appear or just after leaf drop. Good luck!


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InvisibleBunya
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Re: oyster mycelium on agar to dowle. [Re: Quadman]
    #23721093 - 10/09/16 12:24 AM (7 years, 3 months ago)

The Dowel wbs is colinized

I boiled and boiled the dowel for a few hours and some still didnt sink.
So put some in a jar with something holding them under water for a few days and they sunk. Its my prefered method now less watching and fiddling.

Drilled Logs today and inserted dowels and sealed holes with bees wax.


Dowel before being hit in.
.

Then i soaked logs in water for a few hours.
.

Now to keep them moist in the shade watering reguarlly.
And wait a few months to see if it works.
Any thoughts, criticism.


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InvisibleBunya
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Re: oyster mycelium on agar to dowle. [Re: Bunya]
    #23721098 - 10/09/16 12:30 AM (7 years, 3 months ago)

Ive recently done a few more bags of dowels.
Took Ferathers advice with fert and put a few chook poo pellets in with straight dowel and an agar slab.
I must say that this is my prefered method now.
Very quick to colinize.
Its great having the grain left over with the other method to do straw bags but the contam risk is allot higher.


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OfflineFerather
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Re: oyster mycelium on agar to dowle. [Re: Bunya]
    #23721274 - 10/09/16 05:51 AM (7 years, 3 months ago)

Quote:

Bunya said:
Took Ferathers advice with fert and put a few chook poo pellets in with straight dowel and an agar slab.
--
Its great having the grain left over with the other method to do straw bags but the contam risk is allot higher.




Grain is amazing for nutrition, but I hate the attraction to bacteria due to high starch levels.
Starch free, or limited starch, enriched substrates are much safer.


Quote:

Bunya said:
I must say that this is my prefered method now.
Very quick to colinize.




It instantly became my chosen method too.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


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OfflineQuadman
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Re: oyster mycelium on agar to dowle. [Re: Ferather]
    #23721368 - 10/09/16 07:26 AM (7 years, 3 months ago)

:goodluck: lookin good ...... now:popcorn:


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