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Tookitooki
Mycological Fabricator


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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My myco adventure! (GT, Brazilians, Mexicube)
#23613009 - 09/04/16 10:26 PM (7 years, 4 months ago) |
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I've been a long time reader. I had my first experience with cultivation back in 2009. Thanks to the wealth and knowledge, and great folks on this site, it was a successful adventure. I have wanted to post my grows on here for a long time. I still don't know why i didn't do it then, but I'm going to commit to doing it now. i really like where things have gone, with the advancement of teks, knowledge, and weening out of outdated info.
so here it goes........
My multi spore went straight to LC, malt dex. I'm using qt jars this time rather then bags,
golden teachers, mexicubes, Brazilian, and maza, the maza lc was the only one that didn't look right, i think i got a bad syringe. the other three did good. i used all the standard teks to prep the WBS.
5cc LC >WBS i was surprised at the rate of colonization compared to my previous experience. i learned that it was because my previous adventure had a lot of bacteria which hindered its speed and yield potential. I've been on travel a lot lately, so my jars got a few extra days after 100% colonizing.
wbs> coir/verm/gypsum one brick coir, 2 1/4 qt. verm, half qt gypsum
i lined a 5 gal bucket with an oven bag, mixed all my ingredients in, boiled 4 qt. of water and dumped it in, mixed it all around. i put the bags in the oven, set on 170 for 2 hours, then let them sit till i had time to put the monos together. some of the monos got 4 qts some got 5.5.
so here is where we are now
i had 8 jars colonize fairly quick (7days) so i spawned them to 2 monos before the rest were done. they have about a 5 day head start on the other seven that were spawned 8/23-24. the 5 on the right are done colonizing, they were spawned on 8/30. maybe tomorrow or tuesday ill prepare some jiffy mix, case and fruit them.
tub 1

if you look close, a few of the mushrooms are blueing. i tried to take a close up of the worst one. it should be the third pic. I've never come across this before, and I'm seeing it on a few fruits in this tub. i have a feeling something isn't dialed in right.
temps stay around 75 during the day and about 68 at night. this is the only tub i mist, the poly fill fell out the top hole, and it lost all moisture on the walls.so i switched to ez felt instead of polyfil.
tub 2

this is similar to what the other tubs all look like minus this one has more pins and the others just popped pins as of today. do you think this one looks decently dialed in?
update 09/06/16
Tub 1: half harvested, this is what was remaining. Ended up with just over 3oz dried.
 Looking for some pointers on the tubs.
Tub 2 : Am I correct at saying the really thick solid white on the left is bacteria? And that's why my pin set is all to the opposite side?

Tub 3 : I have a handful of pins coming in, but this tub worries me. My camera does not do it justice, but I have some weird green/yellow/brown coloration around the edges. The second pic I think shows it the best. And again on the edges, it's the same dense white spots.

Tub 4 : if you look at the sub near all the holes, it's a little thicker white right there. It's this telling me lack of fae? And after going back and looking at them, all my other tubs are exhibiting the same thing.
I prepared 500ml of agar and poured 24 plates. Some where thicker and I didn't have enough for the 25th plate.
I took the best two looking fruits, from different clusters and transferred some tissue to agar.
update 09/08/16
Tub 1: rehydrated and back in fruiting conditions.
Tub 2 has some bacterial issues going on. only harvested about 45 grams dry off it. This tub was also rehydrated and now back in fruiting conditions.
Tub 3: has been moved to my basement. The funny spots started to develop a powdery look. Not sure if its trich, but it's a contam of some sort.
My agar plates are showing my growth!! So far all but one look good. That one of my fault. A drop of soapy water fell in it lol don't ask. It's very exciting being my first time with agar.
update 09/10/16
Tub 1&2 are awaiting a second flush
Tub 3: I believe the contamination sporulated, hence the powdery look, shortly after yellow metabolites started to show up on all the funny looking spots. No more powdery look. Despite the contam, this has been one of my denser pinsets and flushes.

And this is one of the three harvests of this tub. Once they finish drying I'll post a dry weight

Tub 5: mexicube this is another example of the issues I'm having. this tub was misted before the picture. The sub surface is drying out,t he sub is shrinking, but I have heavy condensation on the walls and lid, and water is pooling under the liner. The fruits are showing signs of unhappiness. I'm trying to learn what there trying to tell me.

Tub 6: mexicube See description to tub 5 in addition I'm noticing the fruits seem to barely be attached to the sub. And some of fruits are blueing bad

Tub: 7 Corumba Brazil. So far this one is looking good. I'm just wondering if I am having a lack of fae.

And these are my agar plates, not the four original, but my second attempt at cloning.

Last night and today, I've been pasteurizing some jiffy mix to case the next five tubs. I'm wondering if this is going to help me with my issues.
update 09/11/16
Tub 1: already pushing it's second flush. Im starting to think my tubs like my musty moldy basement.

Tub 2: waiting second flush
Tub 3: first flush complete got about 3oz dried. Most had small caps and longer stems.
Tub 4: finally putting out some decent fruits. I have it labeled GT, but it's looking more like my mexicubes I have.

Tub 5: mexicubes

Tub 6: mexicubes lackluster pinsets and flush, but I'll get a clone of this and see if I can get a good flush next time

Edited by Tookitooki (09/11/16 06:32 PM)
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Re: My myco adventures, then and now (now GT, Brazilians, Mexicube) [Re: Tookitooki]
#23616838 - 09/06/16 02:09 AM (7 years, 4 months ago) |
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You've got a nice grow going on .) Not sure why nobody commented, looks like you're doing really well.
As I said in the other thread, that blue color is just the result of bruising, nothing to worry about. I'm surprised that you haven't seen it before, considering the grows you mentioned you've done. They also bruise after you pick them, I'm sure you've seen that right? It's the same thing.
Anyway, well done and keep posting .) Have my 5 shrooms
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
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Tookitooki
Mycological Fabricator


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: My myco adventures, then and now (now GT, Brazilians, Mexicube) [Re: Tira]
#23617696 - 09/06/16 10:23 AM (7 years, 4 months ago) |
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Thank you. On another post I made asking about the blueing, they responded that it looked like water damage and lack of Fae at the same time. It makes sense, I had some issues when first setting everything up with these tubs. I was moving everything around a lot and I lost the polyfil out the top hole and it really dried out my sub, so I started misting and that's when the fruits turned up that way. I wasn't able to get a picture this morning of the tub before I harvested. I was running late for work but didn't want to come home to spores dropped everywhere. I'll post an update tonight.
And I put some agar dishes together for the first time last night, and took tissue from two different fruits. I'm a little nervous, as I feel i was a little sloppy with it, in my SAB. I guess we will find out in a day or two
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Tira



Registered: 11/20/10
Posts: 1,202
Loc: Turkey
Last seen: 6 years, 3 months
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Re: My myco adventures, then and now (now GT, Brazilians, Mexicube) [Re: Tookitooki]
#23617704 - 09/06/16 10:25 AM (7 years, 4 months ago) |
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Quote:
Tookitooki said: And I put some agar dishes together for the first time last night, and took tissue from two different fruits. I'm a little nervous, as I feel i was a little sloppy with it, in my SAB. I guess we will find out in a day or two
Haha I'm also doing agar for the first time today, they're in the PC right now. Hope you did well and hope I'll do well .)
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
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Tookitooki
Mycological Fabricator


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: My myco adventures, then and now (now GT, Brazilians, Mexicube) [Re: Tira]
#23617746 - 09/06/16 10:46 AM (7 years, 4 months ago) |
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I know one dish of mine is going to be interesting. I spray my SAB down with soapy water, then I'll lightly wipe it so nothing drips. I guess i didn't wipe enough, and as I was putting my first piece of tissue in the agar, a drop of soapy water fell on the dish.... lol oh well. Hopefully you'll have better luck then me.
And to answer your question, I've seen the normal bruising from handling then our bumping them to hard, I just never had it on undisturbed fruits before
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Tookitooki
Mycological Fabricator


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: My myco adventures, then and now (now GT, Brazilians, Mexicube) [Re: Tookitooki]
#23619675 - 09/06/16 08:39 PM (7 years, 4 months ago) |
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Here is the rest of the first flush. Nothing fancy, but good enough for some prints and hopefully clones. After harvest I cleaned up the sub the best I could and soaked it for two hours, I went to go dump the water out and I put my hand in the middle and pushed, the sub broke in half. Not completely, but most of the way. It's back in fruiting conditions, I guess I'll see what happens.
 Looking for some pointers on the tubs.
Tub 2 : Am I correct at saying the really thick solid white on the left is bacteria? And that's why my pin set is all to the opposite side?

Tub 3 : I have a handful of pins coming in, but this tub worries me. My camera does not do it justice, but I have some weird green/yellow/brown coloration around the edges. The second pic I think shows it the best. And again on the edges, it's the same dense white spots.

Tub 4 : if you look at the sub near all the holes, it's a little thicker white right there. It's this telling me lack of fae? And after going back and looking at them, all my other tubs are exhibiting the same thing.
I attempted my first agar pour last night. I followed all the standard teks, I did the 47°c water bath, when I put my flask in the water bath, it Rose to 55°c, took forever to come back down to 47°c. By the time I was putting the last petri in the sleeve, it was starting to gel back up. Now most of my dishes have condensation in them. I stacked them 8 high in my SAB for about 45 mins. Not sure where I went wrong on this one.
I used 4 dishes for clones, and put the rest back in the sleeve they came in and stacked them on the shelf until my next tub throw it's fruits.
Edited by Tookitooki (09/06/16 08:57 PM)
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