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OfflineAgarStudent
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Mexican on Agar
    #23617332 - 09/06/16 07:49 AM (7 years, 4 months ago)

Hi,

inoculated a plate of agar with a syringe with ps. Mexicana nearly two weeks ago and noticed some light brown spots?  Are these normal?

Thanks guys :smile:



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OfflineTheGODSmadeMe
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Re: Mexican on Agar [Re: AgarStudent]
    #23617599 - 09/06/16 09:45 AM (7 years, 4 months ago)

Damn mexicans on agar...whats the world coming to


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OfflineKenetic
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Re: Mexican on Agar [Re: TheGODSmadeMe]
    #23617635 - 09/06/16 10:00 AM (7 years, 4 months ago)

Its normal if you consider a contaminated plate normal


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OfflineAgarStudent
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Re: Mexican on Agar [Re: Kenetic]
    #23617657 - 09/06/16 10:09 AM (7 years, 4 months ago)

The browny residue on the outside is coffee that was put in the agar.  Don't ask why.
I really meant the bits in the centre of the growth blobs?
I put arrows to point to them
Do they look like a contam?



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Offlinemyceliups
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Re: Mexican on Agar [Re: AgarStudent]
    #23617686 - 09/06/16 10:20 AM (7 years, 4 months ago)

mexicana mycelia can look pretty funky. I would try transferring
some onto some fresh agar and see. You might be good.


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Offlinespacechildo
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Re: Mexican on Agar [Re: AgarStudent]
    #23617708 - 09/06/16 10:27 AM (7 years, 4 months ago)

yeah I'd do a transfer about 7-10 days ago, no point in using agar if you're just gonna let everything germinate.

ps.mexicana arent cubes like myceliop pointed out, browning is normal.


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OfflineAgarStudent
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Re: Mexican on Agar [Re: myceliups]
    #23617711 - 09/06/16 10:28 AM (7 years, 4 months ago)

Ah okay -

Might try transferring -
Heard that if I used coffee the myc would get excited and start producing stones on the plate that I could transfer to another plate, but still learning with this...

Not sure exactly what stones look like in early myc development, but open to suggestion :smile:
RR said something about transferring the darker coloured sectors of myc, but I have not got that far yet, considering this is a syringe squirt shot and not a transfer.

Cheers


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OfflineAgarStudent
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Re: Mexican on Agar [Re: AgarStudent]
    #23617721 - 09/06/16 10:33 AM (7 years, 4 months ago)

Oh thanks Spacechildo -

Yes I wanted to let things germinate so that I could pick the most likely stone-producing bit.
Hopefully can still do this.
The problem before was that I transferred too early and only picked the fastest growing bit of mycelium - not necessarily the best producer of stones.
I ended up with jars that colonised, but did not seem to produce stones, so thought I would start over...  Hopefully transferring a dark browny bit?


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Offlinemyceliups
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Re: Mexican on Agar [Re: AgarStudent]
    #23617723 - 09/06/16 10:36 AM (7 years, 4 months ago)

I would just work with the white mycelia for now. And ignore
the coffee for now. I have heard things about coffee to make
more stones.

If you want to find a variety that produces a bunch of stone I
would noc up a bunch of jars with mexicana MS and let them
sit for 2 months or more. Most of the jars will produce stones.
But doing MS at first will allow you to find more stones IMO.
When you have what you want take a stone culture of the
best specimen and there ya go.


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Offlinespacechildo
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Re: Mexican on Agar [Re: AgarStudent]
    #23617729 - 09/06/16 10:38 AM (7 years, 4 months ago)

yeah but you should still do the 1st transfer pretty soon after you see growth to get away from the contam spores that most likely also sits on your agar.
you can still let the next plates grow out and look for browning myc on them.
also its a good idea to know where you swiped/dropped the spores,either swipe the loop in a Z pattern or make a dent in the center when you cool your loop and put the spores there.
if you simply swiped the print all over the plate you also swipe your contam spores all over the plate, making it hard to know which parts are truly clean and which ones got un-germinated contamspores on them.


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OfflineAgarStudent
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Re: Mexican on Agar [Re: spacechildo]
    #23617740 - 09/06/16 10:44 AM (7 years, 4 months ago)

Oh I didn't swipe the print - I just put a few drops of the syringe liquid onto the plates over the air flow of the laminar flow hood - flame sterilising the needle first of course.
Contam spores shouldn't be in the plate anyway, being done under sterile conditions.
How did you get contam spores?


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Offlinespacechildo
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Re: Mexican on Agar [Re: AgarStudent]
    #23617745 - 09/06/16 10:46 AM (7 years, 4 months ago)

from the print.no prints are 100% clean that's why cleaning growth up on agar is sogreat!
checkout stro'scleaning on agar !


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OfflineAgarStudent
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Re: Mexican on Agar [Re: AgarStudent]
    #23617753 - 09/06/16 10:48 AM (7 years, 4 months ago)

Oh thanks mycellups -

You mean to find the best stone producing jar and just to a grain to grain transfer?

May I ask - If it is pretty well colonised and hard to just tip the jar up to transfer grains, then is it okay to flame sterilise, say a teaspoon or butter knife in order to break up the grain a bit so that I can transfer it, when I tip the jar upside down over the flow hood.

Cheers :smile:


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OfflineAgarStudent
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Re: Mexican on Agar [Re: AgarStudent]
    #23617763 - 09/06/16 10:53 AM (7 years, 4 months ago)

Thanks Spacechildo -

That is a pretty good tek.  I guess if there was a possible contam, it would have been seen by now, but no excuse for not transferring.  Blue mould or cotton mould would have definitely infiltrated by now if they were present?

Cheers


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Offlinemyceliups
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Re: Mexican on Agar [Re: AgarStudent]
    #23617777 - 09/06/16 10:59 AM (7 years, 4 months ago)

Quote:

AgarStudent said:
Oh thanks mycellups -

You mean to find the best stone producing jar and just to a grain to grain transfer?

May I ask - If it is pretty well colonised and hard to just tip the jar up to transfer grains, then is it okay to flame sterilise, say a teaspoon or butter knife in order to break up the grain a bit so that I can transfer it, when I tip the jar upside down over the flow hood.

Cheers :smile:




That should be just fine


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Offlinespacechildo
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Re: Mexican on Agar [Re: AgarStudent]
    #23617886 - 09/06/16 11:37 AM (7 years, 4 months ago)

Quote:

AgarStudent said:
Blue mould or cotton mould would have definitely infiltrated by now if they were present?




if by infiltrated you mean germinated, not necessarily. spores can still be there but they wont germinate on the mexicana myc if it grew over the spores. so X amount of transfers in it may get to germinate on the agar if it just piggybacked along the way.

you can clone a stone, I wouldn't g2g a stone producing jar.


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