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Jaybob



Registered: 05/19/16
Posts: 167
Last seen: 4 months, 27 days
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Questions about 1st grow 8 days in
#23615649 - 09/05/16 06:12 PM (7 years, 4 months ago) |
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Ok i have a few questions. 8 days ago I inoculated 15 1/2 pint jars using Evilmushrooms take on PF... 4 lions mane w sawdust instead of verm

5 pearl oysters

And 6 mex-cub...

The lions mane and oysters were LC and spores for the Mex. Obviously the 1st two are showing growth while the the Mex is showing almost nothing at all. I know LC will grow quicker than spores but
Question 1 - should I be worried about the Mex yet?
I do have 1 tiny little spot on 1 jar

And one definite contam

(I put the tape on when moving it outside to keep anything from getting out on the move) (Yes that's my original Sega genesis in the background)
This morning on 1 of the oysters I noticed the white turning a little bit yellow...

I hope it comes through on that pic. Which leads to...
Question 2 - can you tell me what that is?
That jar has been isolated now as well.
Lastly I tried making more LC w all 3 syringes. Mex showing nothing while other 2 LC's are showing a lot of growth. However, all of what I assume is myc growth settles in the bottom after stirring. Is that normal? All the LC pics I see have myc on top floating.

Thank you for any help and advice
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h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
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Re: Questions about 1st grow 8 days in [Re: Jaybob]
#23615714 - 09/05/16 06:27 PM (7 years, 4 months ago) |
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Agar can be your friend and significantly increase your odds of success compared to working directly from spores. Here's a guide to making up some pasty plates, https://www.shroomery.org/forums/showflat.php/Number/19208976.
You can take the spore syringe and use a drop or two on agar to get a good culture growing. If it contaminates, you transfer until it cleans up.
Then, you what you will end up with is a culture you know is healthy and happy. Which is ideal for making LC, LI, grains, cakes, the possibilities are almost limitless.
Test the LC on a single cake or on agar before proceeding. Going from MS to LC may increase your odds of contamination. If it turns out your LC is contaminated, you do not want to find out after it is too late.
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Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Questions about 1st grow 8 days in [Re: h0ldthedoor]
#23615767 - 09/05/16 06:41 PM (7 years, 4 months ago) |
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is that a sega!? im coming over.
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Jaybob



Registered: 05/19/16
Posts: 167
Last seen: 4 months, 27 days
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Re: Questions about 1st grow 8 days in [Re: h0ldthedoor]
#23615802 - 09/05/16 06:52 PM (7 years, 4 months ago) |
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I've got everything for agar plates including pasty's page bookmarked EXCEPT the GD agar! I've been to every grocery store and whole foods around and 3 different Asian super markets and can't find it anywhere! Gonna have to order online I'm thinking but I can't see if it has added sugar online
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Jaybob



Registered: 05/19/16
Posts: 167
Last seen: 4 months, 27 days
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Re: Questions about 1st grow 8 days in [Re: mushboy]
#23615804 - 09/05/16 06:53 PM (7 years, 4 months ago) |
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He'll yeah it's Sega! With golden axe loaded hahaha.
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h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
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Re: Questions about 1st grow 8 days in [Re: Jaybob]
#23615884 - 09/05/16 07:13 PM (7 years, 4 months ago) |
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If you can find Telephone agar packets at a decent price (<$2), buy a few. If not, post what you are considering purchasing here to get feedback beforehand.
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Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
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Psychedel.EXE
AKA Old Uncle Nutty


Registered: 07/04/16
Posts: 211
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Re: Questions about 1st grow 8 days in [Re: Jaybob]
#23615913 - 09/05/16 07:20 PM (7 years, 4 months ago) |
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Quote:
Jaybob said: (Yes that's my original Sega genesis in the background)
.....sayyyyguh With golden axe too!?! +5
   
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