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InvisibleBoogieman47
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testing clones question?
    #23611283 - 09/04/16 12:41 PM (7 years, 4 months ago)

I'm in the process of testing a few clones I pulled two pe already spawned two mazzie colonizing and two ksss so my question is after a few transfers and you use a piece of agar to colonize grain obviously you have made more plates but what if that strain was killer then the pieces you transfer lose a strain that was beneficial? Because clones are multi strains right ? Is that possible because getting an isolate can give you a single strain that's shit so how would you keep that killer set of genes you tested without losing it? I know I might not make sense I'm a bit dense haha


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InvisibleInocuole
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Re: testing clones [Re: Boogieman47]
    #23611303 - 09/04/16 12:51 PM (7 years, 4 months ago)

I've asked this exact question before.  It could plausibly happen.  Take wider transfers if you'd like to reduce the risk of losing valuable genetics.  Most times though, the dominant genetics that end up getting transferred are still the same ones you cloned for in the first place.  Just keep slants or other backups in case such a thing seems like it's happened.

You'll eventually have to say goodbye to every clone though, so remember to grab spores at one point and try to find it again somewhere among it's progeny.


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InvisibleBoogieman47
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Re: testing clones [Re: Inocuole]
    #23611331 - 09/04/16 12:58 PM (7 years, 4 months ago)

Ya I was just pondering the idea of bigger transfers but what I feel would be shitty is if you waste that killer flush on a small tray when you could have got a bigger flush of another ... but then it could have been shitty and you just wasted sub and time ... I was planning on getting prints of these grows I have coming in and I still have syringes for days but they will be gone haha.. so you're saying if I had a good test and the next one was shit to just make slants of the agar just in case it was a good flush?


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InvisibleInocuole
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Re: testing clones [Re: Boogieman47]
    #23611337 - 09/04/16 01:00 PM (7 years, 4 months ago)

Well you should be making the slants as soon as you're sure the culture is clean from cloning so that you lose as little total genetic information as possible.  If the slant is allowed to fully colonize it'll start to trade genetics with itself via anastomosis and it'll be a bit more homogenous when you go to transfer from it.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisibleBoogieman47
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Re: testing clones [Re: Inocuole]
    #23611352 - 09/04/16 01:08 PM (7 years, 4 months ago)

OK I understand I'm still getting my feet wet with how I do everything I went from my toes and just about to my ankles haha I ordered 25 tubes I have, just been procrastinating ... what about when that good flush comes in you could just clone that just in case? I know you already have the genetics but would that give you a better chance of keeping that good set of genes just in case ?


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Re: testing clones [Re: Boogieman47]
    #23611368 - 09/04/16 01:14 PM (7 years, 4 months ago)

Clones of clones tend to put you a lot closer to the possibility of senescence  than need be.  Weird shit starts happening after enough cell divisions, especially with PE and KSSS.  Assuming that's what you meant.  You can get away with it though, for sure.  I think my best PE clone is a clone of a clone.  It's becoming a little stupid though... about to switch back to some other stuff for a while until I get a new clone.

Either way, don't get too attached to your cultures, shit happens.  Keep prints of the best clones and you'll know finding something good next time will at least be a little easier than last time.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisibleBoogieman47
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Re: testing clones [Re: Inocuole]
    #23611401 - 09/04/16 01:29 PM (7 years, 4 months ago)

OK that makes sense almost like embreeding persay? And I know about getting attached to shit at first I hated throwing agar plates away but I have such an abundance it's pointless and bodhi answered my question about if making slants would be better to save my syringes or keep my syringes as is ... with pe tho you gotta get sterilized qtips for the spores right? And I appreciate the help noc


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