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ninelives
Turd Burglar



Registered: 07/13/15
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Healthy jar?
#23611165 - 09/04/16 12:08 PM (7 years, 4 months ago) |
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Hey guys, started my first 6 jars on August 29th, 4 of them have shown no activity so far, and the other two are slowly progressing. How does this one look like it's doing so far? Since it's the farthest along. I was worried about those small specs. Is that just sub stuck to the side? Doesn't look like mold or bacteria but I thought I'd double check , don't want to risk contaminating my other jars, even if they don't look to promising this go around.

-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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mrmazdarx9
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29th of august there's plenty time yet The pics you posted don't look to bad a bit fluffy so keep an eye on it the first pic looks green substrate probably the light though
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ninelives
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Thanks for the reply, yeh I think the green at the bottom right may be the camera flash, it isn't generally that color but I'm definitely keeping an eye on it. I've seen a lot of your posts so I trust your judgement.
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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Tira



Registered: 11/20/10
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Those look good to me, but why do they have foil on top of them? Don't you have lids with filtered holes on them?
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
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TheEaglesGift
The Nagual


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Re: Healthy jar? [Re: Tira]
#23613641 - 09/05/16 03:15 AM (7 years, 4 months ago) |
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Growth looks good. Your lid set up may or may not be a problem if it's restricting GE.
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mrmazdarx9
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Quote:
ninelives said: Thanks for the reply, yeh I think the green at the bottom right may be the camera flash, it isn't generally that color but I'm definitely keeping an eye on it. I've seen a lot of your posts so I trust your judgement.
Thanks Also are you using foil as a lid or have you got lids and foil if you have lids on then take off the foil its not needed and as said above restricting gas exchange which will slow things down alot
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ninelives
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Its just got tightly sealed foil on it. Each jar has 4 small inoculation holes, I wasn't sure how the syringe would fare against the lids I have. This is that same jar as of today, how is the growth looking? I felt like I had some cobweb, it looks pretty light and fluffy. No off colors though.
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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mrmazdarx9
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Foils fine what i use ive had no problems and great cakes First pic looks a bit weird the rest don't look bad if it was cobweb it would grow 1000 times its size in a day it grows rapidly
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ninelives
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This jar seems to be colonizing at like 3x the speed of the others. The stuff in the first pic kinda threw me off but as far as I can tell it's not colonizing faster than the other side of the jar. The first picture is unique to that one jar, the others jars don't seem to resemble it as much.
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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mrmazdarx9
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Quote:
ninelives said: This jar seems to be colonizing at like 3x the speed of the others. The stuff in the first pic kinda threw me off but as far as I can tell it's not colonizing faster than the other side of the jar. The first picture is unique to that one jar, the others jars don't seem to resemble it as much.
There are a lot of things that can cause this usually bacterial jars it depends how you look at it is that jar 3x faster or is in normal and the others are 3x slower Looking at the pic from the other day it doesn't look like its going particularly rapid looks normal over a couple days
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ninelives
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The other ones are going particularly slowly so that may be it. Is the mycelium just thinner in picture 1 or should I quarantine it? Everything has been extremely sterile to my knowledge, I went in and nuked the grow room a day before inoc., watching where I breath, only entering the grow room after a shower with clean clothes, etc..
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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mrmazdarx9
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I means its weird looking, I haven't seen anything like that. So it could just be weird growth. I wouldn't quarantine it, the dry verm layer should keep anything nasty that might be lurking in there. Just let it do its business and try not to bother it for as long as you can. The more you move it the more the dry verm layer moves and could potentially swish to one side leaving an area slightly exposed. I've had a jar go bad right where the verm layer met the sub, so that was my thinking, as I struggle to not mess with them multiple times a day lol
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ninelives
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Not looking has been the hardest thing so far haha, but I'll definitely limit my contact with them. You've been a ton of help man I really appreciate all the info. Normally I'd be pulling my hair out trying to find something wrong with it.
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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TheEaglesGift
The Nagual


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Patience is definitely a virtue.
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mrmazdarx9
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Quote:
TheEaglesGift said: Patience is definitely a virtue.
Definitely  The best thing to do is start other projects like agar, learn the principles find the equipment build Sab and if you haven't already sgfc not for agar but for cakes its needed Make some cultures get a clean strong plate of myc then you'll have a never ending supply all before your first flush on the cakes
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TheEaglesGift
The Nagual


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Not to get off topic, but yes, learning agar is an extremely wise decision.
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ninelives
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So this is my progress so far, I threw out a jar today which was very obviously contaminated, but I'm a little worried about my other five. This first jar here has a picture from 3 angles, which so far looks okay, though I am concerned about a spot on the third picture.

 The spot up top by the foil looked slightly funny to me, still from jar 1.

These are jars 2 & 3 which seem to be okay

Jars 4 and 5 seem iffy to me, but I can't be sure.

Thanks for everyone's help I really appreciate all the input I've received from y'all.
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
Edited by ninelives (09/12/16 12:14 PM)
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ninelives
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Any input on if I should be quarantining the last two jars? Or any of that matter. Thanks guys
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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Tira



Registered: 11/20/10
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They all seem kinda bacterial to me, no need to quarantine them though, just don't mess with their lids and keep waiting.
-------------------- Useful Links for Beginners The Basics AMU Teks Frank''s Teks Agar Noob Forum Reccomended Teks Agar for guaranteed spawn, Proper pasteurization for guaranteed substrate.
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mrmazdarx9
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Re: Healthy jar? [Re: Tira] 1
#23640685 - 09/13/16 05:55 AM (7 years, 4 months ago) |
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As I said before leave them alone the more you mess with them the more likely they'll contaminate and the more you stare at them the more worried you'll get yourself
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TheEaglesGift
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Start up a new project. Worst case scenario, you have a new set of jars that make it if these don't. Best case scenario, you have a lot of jars to fruit.
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ninelives
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So I walked by my grow room, and without entering I catch a whiff of this rancid milk odor, so I go in, and smell each jar without disturbing or touching them. The rancid smell was pretty dang potent, but it was only in one jar, so it's been removed and discarded. Are my other jars at risk, since they were basking in the stank? I went ahead and started another set of jars, thanks @TheEaglesGift. I might have an idea of what went wrong. Ran out of solution on my last two jars, so I sucked a small amount of filtered drinking water in the syringe, one turned black, the other, the gnarly smell. Is this stuff naturally in my drinking water? Is boiling the only way to achieve truly sterile water?
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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ninelives
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@Mrmazdarx9, promise I didn't touch lol :P. The jar on the right is the trouble maker. I left it outside and it looks like it's putting up a valiant effort to survive, curious to see what happens. The BRF/Verm was looking slimy.
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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Mad Season
hookers and blackjack



Registered: 09/16/12
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Ew. Wet and clumpy af! Hella bacterial. Those look perfect for the trash can. Take off the foil during colonization.. also what recipe did you follow? It should be 1:1:2 water:brf:verm. Water and verm mixed first and then brf mixed in after.
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ninelives
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I used FungiFun's PF Tek For Simple Minds. My sterilization process was almost hypochondriac like. I had a slight cold when I made them, but I even went as far as wearing a disposable face mask. Ive got them in a closet that stays at 79 regardless of the thermostat. It was completely sterilized and emptied, shampood carpet, Lysol on walls, counter and ceiling. My BRF/Verm ratio was spot on as well. No odors from the other jars though, and growth is pretty good at the moment. Does odor mean it's gone airborne? Or is it just odor from the bacteria digesting?
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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mrmazdarx9
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Quote:
ninelives said: @Mrmazdarx9, promise I didn't touch lol :P. The jar on the right is the trouble maker. I left it outside and it looks like it's putting up a valiant effort to survive, curious to see what happens. The BRF/Verm was looking slimy.

Lol it happens I just binned a PE jar that I was a little unsure of, wasn't the same as the rest of the jars. Normally I'd of let it finish but I've learnt its generally not worth the wait or the risk of contaminating the rest
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ninelives
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The smell was pretty gnarly. Held my breath on my way outside it was so bad. It was the farthest behind, so I wasn't too bummed about it. Would you recommend wide mouth half pint jars instead?
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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TheEaglesGift
The Nagual


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What kind of water did you suck into the syringe? Bottle distilled water is usually pretty safe I hear, but I've always used PC sterilized or at the very least boiled water when working with spore syringes.
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ninelives
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Used filtered water from my fridge, which was purchased recently, but I've got a feeling that was the problem. Four out of seven of my jars seem to be pretty healthy, and I inoculated 3 of them with drinking water, so I'm assuming those are the jars that failed. I didn't label them though (dumb I know), so I can't be sure. Last night I made two jars of just drinking water using a spare unopened syringe, following the exact process, without using spores to see if anything nasty grows.
Working on my SGFC today. Using an old PC fan mounted to the side for FAE. The rest of my fans are industrial, so that's a no go with those.
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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ninelives
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The contaminated jar I placed outside is really fighting, even with the heat. The smell has diminished as well but the Mycelium fights on.
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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mrmazdarx9
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Quote:
ninelives said: The contaminated jar I placed outside is really fighting, even with the heat. The smell has diminished as well but the Mycelium fights on.
I like to do that the myc usually wins even had one go mostly green then the myc took it back, I never used it though just buried it once I got bored
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ninelives
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I figured it deserved a fighting chance. I felt too guilty throwing it away, even though it's unusable. I'm going to give it some time then bury it. Unfertilized manure okay? I've got loads of it.
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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mrmazdarx9
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Quote:
ninelives said: I figured it deserved a fighting chance. I felt too guilty throwing it away, even though it's unusable. I'm going to give it some time then bury it. Unfertilized manure okay? I've got loads of it.
Its basically being thrown anyway
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ninelives
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Alright haha I won't waste my manure on it then, even though I don't have anything else to do with it. Would a Mercury vapor bulb be effective at speeding up the colonization on my other jars?
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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TheEaglesGift
The Nagual


Registered: 04/10/11
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Quote:
Used filtered water from my fridge, which was purchased recently, but I've got a feeling that was the problem.
It was a problem, for sure. Hard to say if this was the cause as you may have made other mistakes, but don't use this as a water source again. Sterilized water is best, though some use boiled or bottle distilled.
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ninelives
Turd Burglar



Registered: 07/13/15
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So am I drinking this stuff on a daily basis? I have an extra mercury vapor bulb that emits UVB for the absorption of Vitamin D in lizards, would this affect the colonization time if used indirectly on a 12/12 schedule?
Also what do you think about Damions Coir Tek on my first grow?
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
Edited by ninelives (09/18/16 06:23 PM)
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TheEaglesGift
The Nagual


Registered: 04/10/11
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Did you think your carbon block filter completely sterilized your water? It doesn't, but that's not really an issue for drinking but it is an issue for mycology. The housing units for those filter are usually filled with massive biofilms unless they're cleaned. The carbon block itself will become a breeding ground for biofilms if they're not changed often enough. That said, it hasn't killed you yet. Drinking water isn't supposed to be sterile, it just shouldn't have pathogens, which chlorine and processing takes care of. Wells and especially springs can be safe and great water sources as well. That said, chlorine and the other constituents in city water are horrible for your health. The inorganic minerals in well water are damaging as well. I suggest using a water distiller if you have city water or hard well water(most well water is very hard). This is getting pretty off topic but considering it's your thread I don't see the issue.
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ninelives
Turd Burglar



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It makes sense that it doesn't filter everything, though the last thing I would have expected to be present in it was bacteria. Colonization is moving along, slowly but surely in last four jars. I'll let you guys know how everything works out. Sorry about this getting so far off topic, I really appreciate all the help though, I've learned a ton from this thread.
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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ninelives
Turd Burglar



Registered: 07/13/15
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Last seen: 5 years, 8 months
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Progress so far. Looks weird to me but we'll see.
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
Edited by ninelives (09/19/16 06:07 PM)
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mrmazdarx9
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Bacterial but should still fruit, not long now till birthing time couple weeks maybe not forgetting consolidation
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ninelives
Turd Burglar



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I figured, I started another 5 jars so I'm not too disappointed. Thanks for all your help man, it really made an impact on the experience of my first grow!
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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mrmazdarx9
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Quote:
ninelives said: I figured, I started another 5 jars so I'm not too disappointed. Thanks for all your help man, it really made an impact on the experience of my first grow!
Most cakes are bacterial I wouldn't worry too much, let them finish you'll get get your reward
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ninelives
Turd Burglar



Registered: 07/13/15
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Thanks man  One is already fully colonized, I'm going to birth it next Thursday
-------------------- You wish to grow but fear what you may become, so you push off the water, the dirt, the sun, to stay in your hole. But it is in the very pushing that you become a tree, in spite of yourself. How did that happen?
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mrmazdarx9
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Don't forget 24hour dunk and then roll in dry verm. After this I leave my cakes in the sgfc for half a day or so before fanning and spraying to give it time to stick the verm to the myc
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