Home | Community | Message Board


Magic-Mushrooms-Shop.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Agar, Impulse Sealer, Laminar Flow Hood, Wild Bird Seed

Jump to first unread post. Pages: 1
InvisibleStix Russell
AmorphousExperimentalist

Registered: 11/27/99
Posts: 113
H2O2 use with spore syringes instead of agar?
    #2360916 - 02/20/04 09:29 PM (13 years, 6 months ago)

The grain is strained, rinsed and dried thoroughly before being loaded into the quart jar, ? full. The lid has a filter disc in it, but polyfill, or even coffee filters or cardboard can be substituted since we will be adding peroxide later. The jar is pressure cooked to sterilize and also destroy peroxide decomposing enzymes. When cool, the quart is injected with 12 cc?s peroxide, shaken and inoculated in front of a flow hood with a piece of the peroxidated agar. Colonization is complete in a week with temps around 80 deg F. A huge filter patch bag is prepared with birdseed as above. Peroxide amount is proportional to that listed above. In the flow hood, the bag is inoculated with the colonized quart jar and sealed with an impulse sealer. The bag is colonized in 11 days at room temps.

Could I get some insight into the above method listed as the bulk neglect report utilizing inoculation via a spore syringe instead of colonized agar? Do you think the introduction of H2O2 would harm the spores' probability of germination as opposed to the effect it apparently does not have on the colonized mycelium in the agar medium?

I attempted a search, but did not find my answers.


--------------------
Where is the lightning to lick you with its tongue? Where is the madness with which you should be cleansed? Behold, I show you the Superman. He is this lightning, he is this madness.

Nietzsche (in Zarathustra)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBaby_Hitler
Errorist
 User Gallery

Folding@home Statistics
Registered: 03/06/02
Posts: 22,844
Loc: To the limit!
Last seen: 15 days, 23 hours
Re: H2O2 use with spore syringes instead of agar? [Re: Stix Russell]
    #2360958 - 02/20/04 09:46 PM (13 years, 6 months ago)

Peroxide kills spores dead. You cannot use peroxide with spores at all.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemuse_sick
ĤŌĿŶĞЋ0$Ŧ

Folding@home Statistics
Registered: 12/15/03
Posts: 9,399
Loc: Giu La Testa
Re: H2O2 use with spore syringes instead of agar? [Re: Stix Russell]
    #2360959 - 02/20/04 09:46 PM (13 years, 6 months ago)

to do bulk w/o agar
just colonize yr substrate (pf jars, wbs, millet, whatever)
then spawn to poo/straw
the agar in the bulk neglect report
is to maximize yields (growing from a clone)
so you don't need agar, it just helps.


--------------------
:beakedwhale:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleStix Russell
AmorphousExperimentalist

Registered: 11/27/99
Posts: 113
Re: H2O2 use with spore syringes instead of agar? [Re: Baby_Hitler]
    #2360962 - 02/20/04 09:48 PM (13 years, 6 months ago)

Quote:

Baby_Hitler said:
Peroxide kills spores dead. You cannot use peroxide with spores at all.




I assumed it might be so. Thanks for the confirmation.


--------------------
Where is the lightning to lick you with its tongue? Where is the madness with which you should be cleansed? Behold, I show you the Superman. He is this lightning, he is this madness.

Nietzsche (in Zarathustra)


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Agar, Impulse Sealer, Laminar Flow Hood, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Agar Agar peroxide jellyfish 1,075 5 07/20/06 11:59 PM
by fastfred
* Swiping Spores Across Agar. Lines vs. Circles? tahoe 728 1 01/29/09 12:05 PM
by RogerRabbit
* Spores on agar question .More info plz tripndicular 1,100 5 09/13/02 07:27 PM
by tripndicular
* agar/peroxide conc. iudexk 569 1 08/27/01 03:17 PM
by jonnyshaggs420
* Newbie about to dive in head first - amount of spore syring CCs into 4 lb grow bag?
( 1 2 all )
ChrisHippyLoser 11,767 25 06/02/16 07:47 PM
by OldSawbonesHisself
* Agar & Peroxide Question oddiseus 957 7 02/22/05 06:25 PM
by oddiseus
* Getting ready to make my first spore syring fromthemoon 7,155 10 06/29/07 10:49 PM
by wikedferret
* spore print--> agar substitute
( 1 2 all )
Preston 3,606 28 11/29/15 04:10 PM
by cdmc1984

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, RogerRabbit, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
743 topic views. 26 members, 96 guests and 28 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Myco Supply
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.033 seconds spending 0.006 seconds on 19 queries.