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InvisibleStix Russell
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Registered: 11/27/99
Posts: 113
H2O2 use with spore syringes instead of agar?
    #2360916 - 02/20/04 09:29 PM (13 years, 2 months ago)

The grain is strained, rinsed and dried thoroughly before being loaded into the quart jar, ? full. The lid has a filter disc in it, but polyfill, or even coffee filters or cardboard can be substituted since we will be adding peroxide later. The jar is pressure cooked to sterilize and also destroy peroxide decomposing enzymes. When cool, the quart is injected with 12 cc?s peroxide, shaken and inoculated in front of a flow hood with a piece of the peroxidated agar. Colonization is complete in a week with temps around 80 deg F. A huge filter patch bag is prepared with birdseed as above. Peroxide amount is proportional to that listed above. In the flow hood, the bag is inoculated with the colonized quart jar and sealed with an impulse sealer. The bag is colonized in 11 days at room temps.

Could I get some insight into the above method listed as the bulk neglect report utilizing inoculation via a spore syringe instead of colonized agar? Do you think the introduction of H2O2 would harm the spores' probability of germination as opposed to the effect it apparently does not have on the colonized mycelium in the agar medium?

I attempted a search, but did not find my answers.


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Where is the lightning to lick you with its tongue? Where is the madness with which you should be cleansed? Behold, I show you the Superman. He is this lightning, he is this madness.

Nietzsche (in Zarathustra)


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OfflineBaby_Hitler
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Re: H2O2 use with spore syringes instead of agar? [Re: Stix Russell]
    #2360958 - 02/20/04 09:46 PM (13 years, 2 months ago)

Peroxide kills spores dead. You cannot use peroxide with spores at all.


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Invisiblemuse_sick
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Re: H2O2 use with spore syringes instead of agar? [Re: Stix Russell]
    #2360959 - 02/20/04 09:46 PM (13 years, 2 months ago)

to do bulk w/o agar
just colonize yr substrate (pf jars, wbs, millet, whatever)
then spawn to poo/straw
the agar in the bulk neglect report
is to maximize yields (growing from a clone)
so you don't need agar, it just helps.


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InvisibleStix Russell
AmorphousExperimentalist

Registered: 11/27/99
Posts: 113
Re: H2O2 use with spore syringes instead of agar? [Re: Baby_Hitler]
    #2360962 - 02/20/04 09:48 PM (13 years, 2 months ago)

Quote:

Baby_Hitler said:
Peroxide kills spores dead. You cannot use peroxide with spores at all.




I assumed it might be so. Thanks for the confirmation.


--------------------
Where is the lightning to lick you with its tongue? Where is the madness with which you should be cleansed? Behold, I show you the Superman. He is this lightning, he is this madness.

Nietzsche (in Zarathustra)


Post Extras: Print Post  Remind Me! Notify Moderator
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