|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
|
Some plates
#23607042 - 09/03/16 06:51 AM (7 years, 4 months ago) |
|
|
Inoculated 0 with a loop using a spore print about a week ago, showed growth slightly smaller than what was photographed today, yesterday. Yesterday, a small portion of the growth was taken and placed on fresh plates (1-3). On number two, a small chunk of agar fell from the blade and that is why there are two spots of growth.
0

1

2

3

There are a couple dozen plates sitting that were inoculated along with 0, although they have yet to show signs of healthy growth. There is one plate that has some funky stuff in the center and white growth around the edges, keeping an eye on it to see if the outside growth becomes viable for a transfer.
Pics are still potato, but hopefully improving..
Any advice, thoughts, feedback is appreciated as always!
Edit: forgot to number photographs
--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
|
Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
|
|
Yeah baby
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
|
happyherb
Stranger
Registered: 04/26/16
Posts: 61
Last seen: 7 years, 2 months
|
Re: Some plates [Re: Kenetic]
#23607118 - 09/03/16 07:44 AM (7 years, 4 months ago) |
|
|
|
spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
|
|
Did you flame your loop prior to use?
|
h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
|
|
Flame to red hot, clean agar to cool and make mark, get some mycelium, place on clean agar, repeated until all three plates had been inoculated. 
Oh, and a loop was used for spores to agar on plate 0 and a blade was used for the transfers to plates 1-3.
--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
Edited by h0ldthedoor (09/03/16 09:28 AM)
|
spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
|
|
Thanks for that just getting into agar and trying to get a nice over head plan
|
h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
|
|
Taken a few minutes ago, spotting some rhizomorphic growth now
0-a

0-b

1-a

1-b

2-a

2-b

3-a

3-b
--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
|
h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
|
|
Taken a little bit ago. Transfers were made from plates 1-3 to plates 4-6. Plate 0 seems to be doing well after the transfer.
0

1

4

2

5

3

6

Anything look viable for LC?
--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
|
|
i REALLY need to find the link but this guy took a colonized plate and put it in a jar of water and shook it like crazy and used that as his LI.
|
h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
|
Re: Some plates [Re: mushboy]
#23630812 - 09/09/16 10:06 PM (7 years, 4 months ago) |
|
|
Plate 3 grew out nicely. The growth almost reached the edge of the glass jar. Earlier, the plate (1/2 pint widemouth mason jar) and prepared LC jar were moved to the SAB. The plate was opened and the agar was broken apart (since the container was glass, getting it to plop out was impossible) using a flame sterilized blade. The LC jar was opened and the agar chunks were dropped into the LC jar.
Playing with some other plates while the LC sits. Once it's ready some will go on agar to check for contaminates. If it is contaminated, the LC will be dumped and by that time hopefully another culture will be ready for transfer to LC. The LC looks a little like wildfire when illuminated. Photos to come.
--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
|
Panarchist
Cyanescendant



Registered: 04/02/16
Posts: 432
Loc: Copeland
Last seen: 7 months, 17 days
|
Re: Some plates [Re: mushboy]
#23633978 - 09/10/16 09:13 PM (7 years, 4 months ago) |
|
|
Quote:
mushboy said: i REALLY need to find the link but this guy took a colonized plate and put it in a jar of water and shook it like crazy and used that as his LI.
in Munchauzen's video series there's one that covers that: https://www.shroomery.org/forums/showflat.php/Number/21032261#21032261
--------------------
|
Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
|
|
Looking good man
|
just_curious
Cultivator


Registered: 03/15/14
Posts: 1,225
Loc: 'Merica (more spcifically...
Last seen: 1 year, 6 months
|
|
Start working on isolating. I wish I would've done that when I first started. After a few transfers you will start to see sectoring of different strains. Take a piece if agar from the fastest growing, healthiest looking strains and put to a new agar plate. Repeat the process until you have uniform growth. Also, you may want to switch up your agar recipe every now and then when doing so. Good luck man.
|
mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 17 hours
|
|
Quote:
h0ldthedoor said: Flame to red hot, clean agar to cool and make mark, get some mycelium, place on clean agar, repeated until all three plates had been inoculated. 
Oh, and a loop was used for spores to agar on plate 0 and a blade was used for the transfers to plates 1-3.
Are you using a loop for agar transfers? I only use mine for innoxulating from spore print.
To make a transfer to a new plate, use a scalpel instead. Take a tiny wedge
--------------------
 Amateur Mycologists United AMU Q&A
|
h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
|
|
Quote:
mushpunx said:
Quote:
h0ldthedoor said: Oh, and a loop was used for spores to agar on plate 0 and a blade was used for the transfers to plates 1-3.
Are you using a loop for agar transfers? I only use mine for innoxulating from spore print.
To make a transfer to a new plate, use a scalpel instead. Take a tiny wedge
Yes, exactly as you described. Except using an xacto knife in lieu of a scalpel. May check locally for a scalpel soon, though. Wondering if the flaking/corrosion/whatever that is showing up on the blade when flamed will not be as bad with a proper scalpel.
Taking photos of the progress shortly.
Just re-read that, no not using a loop for transfers. Only for spore to agar.
--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
Edited by h0ldthedoor (09/11/16 10:06 AM)
|
NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
|
|
I use my loop for taking pulls from slants. My loop is 20 gauge kanthal vape wire attached to a scalpel handle and it works really well. Haven't used the electric one since I built the flowhood lol.
Also exacto knives are cool but I highly recommend getting a scalpel because you can get huge packs of blades for cheap. I use this scalpel with these blades.
Anyway, I wouldn't try making an LC with any of those yet. I'd do 2-3 more transfers of the best looking one.
Edited by NumeroEno (09/11/16 11:19 AM)
|
h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
|
|
Wow that's really economical! Thanks for the tip! 
Yeah, the LC is more for practice and learning than anything, especially practicing getting the LC on agar right, when checking for contaminates. Last time working from spore syringe to agar, the plunger stuck and blew more liquid than intended on the plate. Those looked gnarly after a little bit
0-a
0-b
1

2

lc from 3

4
5

6

Clone attempts from wild fresh cube
--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
|
NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
|
|
They have wild cubes beyond The Wall? No wonder the wildlings are so fuckin crazy lol.
--------------------
Let it grow! Let it grow! Greatly yield! What shall we say, shall we call it by a name As well to count the angels dancing on a pin Water bright as the sky from which it came And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
|
Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
|
|
I have been following this closely. Definitely studying hard here because I'm getting 4 ounces of agar tomorrow, which will be immediately used to start a bunch of plates. Right now I have no cultures or clones, but I do have some B+ and some AA+ spores on the way. Was hoping to get Mexicube to, but not sure that's going to happen. Think the trader botched on me. Can't wait till tomorrow!
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
|
h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
|
|
Out of room for pics on here, imgur couldn't keep the order right, cbf to re-arrange, the captions on the images are accurate.
- 0&18 - Inoculated from spores using loop
- 1,2,6 - Transfered from 0
- 7,8,9 - Transfered from 1
- 13&14 - Transfered from 2
- 10,11,12 - Transfered from 3
- 15,16,17 - Transfered from 5
- 19-22 - Transfered from 18
http://imgur.com/a/uw845
A couple of the plates pictured are being moved to refrigeration and testing the LC on agar is next.
Got 6 qt jars + one partial qt of wbs prepped using this tek https://www.shroomery.org/forums/showflat.php/Number/23187393 on-hand now. Oh and one gallon of reclaimed wbs water for things.
Feedback is appreciated!!!
--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
|
Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
|
|
Hey htd, if you're going to work with agar, I highly suggest you use petri dishes. They're the proper tool for the job. They're also easier to photograph, even with a shitty phone camera.
|
Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
|
|
Pasty plates do the same thing petri dishes do.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
|
morty422
Wuba-luba-dub-dub!



Registered: 07/06/16
Posts: 988
|
Re: Some plates [Re: Kenetic] 1
#23657472 - 09/19/16 12:27 AM (7 years, 4 months ago) |
|
|
Quote:
kenetic said: Pasty plates do the same thing petri dishes do.
Petri dishes have a few pros... but they are not the 'proper tool for the job'...
I have Petri's, 1/2 pint wide mouths, and PP5's all in rotation. They all work the same way.
|
spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
|
|
Quote:
morty422 said:
Quote:
kenetic said: Pasty plates do the same thing petri dishes do.
Petri dishes have a few pros... but they are not the 'proper tool for the job'...
I have Petri's, 1/2 pint wide mouths, and PP5's all in rotation. They all work the same way.
Lol then what is the proper tool for the job.
|
morty422
Wuba-luba-dub-dub!



Registered: 07/06/16
Posts: 988
|
Re: Some plates [Re: spore-ty] 1
#23657542 - 09/19/16 01:13 AM (7 years, 4 months ago) |
|
|
The 'proper tool for the job' is a vessel that allows you to place a pool of media into it that allows mycelium to grow in it contaminate-free. This could be: Plastic Dishes Pyrex Dishes Wide-Mouth Mason Jars PP5 "Mini-Rounds" etc...
If you tell me that you can get "better growth" from a petri than anyone else could from the same culture in the same type of agar solution from the bottom of a wide mouth 1/2 pint than I'm going to laugh at you.
We're talking about a container that holds a volume of matter.... Nothing more.
---------
Now I WILL tell you that I have a lot easier of a time seeing growth through a petri than I do my other agar vessels. But, all of them are exactly the same. They all grow the same.
Pros and Cons.
|
morty422
Wuba-luba-dub-dub!



Registered: 07/06/16
Posts: 988
|
|
Quote:
h0ldthedoor said:
5

Sort of hard to see it while it's through the wall of the container, but it looks nice. I'd keep my eye on that one!
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
|
Quote:
Mycolorado said: Hey htd, if you're going to work with agar, I highly suggest you use petri dishes. They're the proper tool for the job. They're also easier to photograph, even with a shitty phone camera.

nice plates mang!
those reishi plates look like cubensis though. why are they sectoring? is it MS?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
|
|
Quote:
morty422 said:
Quote:
h0ldthedoor said:
5

Sort of hard to see it while it's through the wall of the container, but it looks nice. I'd keep my eye on that one!
Here's a more recent pic of the plate, from the album uploaded last night. Can't wait for my storage to "reset" or whatever so the pics can go up here, where they look much better in a post and retain their original order.
--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
|
mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
|
|
Good work man your skills are coming along nicely
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
|
man, thats a lot of plate pictures. when u have a post with a lot of text, or a lot of pictures (unless its a grow log), few people will actually respond to each point (few will actually read it all either). make ur posts concise and to the point with the highest quality pictures you can. after a few replies, u can always refine ur questions and ask more according to the answers people replied with.
not sure about the site "resetting" storage?? is that a real thing?
just a tip if ur using crappy phone camera (sometimes its all we got)…take a few shots of the plate, but before uploading them to imgur or the site, check them out. if they aren't clear, don't post them. this might be hard with plates, but if i have to use phone camera, i do it outside in natural light for better quality. maybe try a window.
with my regular camera, any light is fine
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
|
|
Yeah, storage limits are a real thing here on Shroomery.org, .
However, as noted in the screenshot above, supporters enjoy unlimited storage space.
Yes, a flagship smartphone camera was used for the shots. Pro-am equipment was used first, but the shots were 'times' worse than the photos taken with the smartphone camera. As someone still new to the hobby, the decision as to whether or not to include photos based on their quality, has been omitted entirely from the posting process. With the rationale being, it's better to include them all for inspection, rather than foolishly remove a photo(s) that other users could have gleaned additional information from.
Sure, opening the lid and snapping a few shots would result in higher quality photos; but doing so remains a clumsy and awkward process. Until more practice has been had with plates that don't matter, shooting this way (One shot through the lid on top, the next through the side of the tub) is the only method this user is comfortable with. Oh and these photos were taken in front of a large window, in a room filled with natural light, a CFL 60w (equivalent) over the makeshift excuse of a photobox and a 5 LED flashlight aimed at one of the walls to refract light at the plate being photographed.
--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
|
Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
|
|
Quote:
morty422 said:
Quote:
kenetic said: Pasty plates do the same thing petri dishes do.
Petri dishes have a few pros... but they are not the 'proper tool for the job'...
I have Petri's, 1/2 pint wide mouths, and PP5's all in rotation. They all work the same way.
Petri dishes are the tool for the job, otherwise every lab in the world would be using your wide mouth half quarts or whatever vessel they had laying around. Petris are much easier to work with, are low-profile, stack nicely, allow for easier transfers to and from and reduce the risk of contamination due to bad technique (low pro -you don't have to reach down into it like you do a higher-walled vessel. Look, OP asked for input and I shared. When you respond with shit like you did, it sounds like you just want to argue as opposed to contribute. If you wanna drive screws with a hammer, knock yourself out.
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
|
yes…i understand storage limits. but what is this "reset"? i think u need to delete pictures to make space instead?
i don't think u should open the lid of the container….i never said that. maybe if u already did the xfer.
not sure what to tell you about the phone camera. i struggle with good shots too unless completely outside.
Quote:
Yes, a flagship smartphone camera was used for the shots. Pro-am equipment was used first, but the shots were 'times' worse than the photos taken with the smartphone camera. As someone still new to the hobby, the decision as to whether or not to include photos based on their quality, has been omitted entirely from the posting process. With the rational being, it's better to include them all for inspection, rather than foolishly remove a photo(s) that other users could have gleaned additional information from.
your grammar and punctuation remind me a lot of ham loaf. you use commas in weird places.
anyways, u could also make the argument that the act of uploading blurry photos is just as "foolish" as uploading them with the very small chance someone could make something out, and that that "someone" was knowledgeable enough to tell you whats going on with them.
all im suggesting is that a clear photo will get you better answers, and will be more helpful to others reading the thread, so the short amount of time u need to spend"screening" them before uploading is worth it.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
|
|
Quote:
blindingleaf said: yes…i understand storage limits. but what is this "reset"? i think u need to delete pictures to make space instead?
i don't think u should open the lid of the container….i never said that. maybe if u already did the xfer.
not sure what to tell you about the phone camera. i struggle with good shots too unless completely outside.
Quote:
Yes, a flagship smartphone camera was used for the shots. Pro-am equipment was used first, but the shots were 'times' worse than the photos taken with the smartphone camera. As someone still new to the hobby, the decision as to whether or not to include photos based on their quality, has been omitted entirely from the posting process. With the rational being, it's better to include them all for inspection, rather than foolishly remove a photo(s) that other users could have gleaned additional information from.
your grammar and punctuation remind me a lot of ham loaf. you use commas in weird places.
anyways, u could also make the argument that the act of uploading blurry photos is just as "foolish" as uploading them with the very small chance someone could make something out, and that that "someone" was knowledgeable enough to tell you whats going on with them.
all im suggesting is that a clear photo will get you better answers, and will be more helpful to others reading the thread, so the short amount of time u need to spend"screening" them before uploading is worth it.
 Can't make out much looks like good growth but I can only assume its myc. I started with petri dishes, in my opinion the better way to start, because if your good at pouring on Petri's with no contams from the beggining then that's one less thing to worry about if and when you move to Petri's. Pasty plates are great noob tools but a lot of pp5 containers are difficult to see through
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
|
Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
|
|
Quote:
blindingleaf said:
Quote:
Mycolorado said: Hey htd, if you're going to work with agar, I highly suggest you use petri dishes. They're the proper tool for the job. They're also easier to photograph, even with a shitty phone camera.

nice plates mang!
those reishi plates look like cubensis though. why are they sectoring? is it MS?
That's just what Reishi does in my experience:
 
I guess it's possible we both have MS cultures, but mine came from a seller who said it's an isolate.
Edited by Greg (09/19/16 09:50 AM)
|
mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
|
Re: Some plates [Re: Greg]
#23658267 - 09/19/16 09:39 AM (7 years, 4 months ago) |
|
|
Lol I did my t1 transfers on 3rd of sept
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
|
h0ldthedoor
HODOR



Registered: 06/25/16
Posts: 510
Loc: North of The Wall
|
|
Quote:
blindingleaf said: yes…i understand storage limits. but what is this "reset"? i think u need to delete pictures to make space instead?
i don't think u should open the lid of the container….i never said that. maybe if u already did the xfer.
not sure what to tell you about the phone camera. i struggle with good shots too unless completely outside.
Quote:
Yes, a flagship smartphone camera was used for the shots. Pro-am equipment was used first, but the shots were 'times' worse than the photos taken with the smartphone camera. As someone still new to the hobby, the decision as to whether or not to include photos based on their quality, has been omitted entirely from the posting process. With the rational being, it's better to include them all for inspection, rather than foolishly remove a photo(s) that other users could have gleaned additional information from.
your grammar and punctuation remind me a lot of ham loaf. you use commas in weird places.
anyways, u could also make the argument that the act of uploading blurry photos is just as "foolish" as uploading them with the very small chance someone could make something out, and that that "someone" was knowledgeable enough to tell you whats going on with them.
all im suggesting is that a clear photo will get you better answers, and will be more helpful to others reading the thread, so the short amount of time u need to spend"screening" them before uploading is worth it.
Apparently, after checking, it looks like there is only 1,200 KB of space left for photos, total. It also appears the limit will not increase. Deleting photos is the only method to increase free space, unless a supporter status is attained.
Did not mean to give the impression that you suggested to open the lid. This is something that was mentioned in the past as another way to get more clear photos.
Unfortunately, taking photos outside is not viable at this time.
The reference to "ham loaf" is lost, not sure as to what you mean. Yes, uploading clear photos is preferred. Unfortunately, it's not always possible to take photographs that reflect the desired level of clarity/focus. Petri dishes are ideal for photographing due to their design. However, petri dishes can be difficult to source locally.
For what it's worth, it's so much easier to see in person, than through the lens of a camera. Though it's unlikely anyone wants to drop by for a house call,
--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you. – Petyr Baelish
|
Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
|
Re: Some plates [Re: Greg]
#23658586 - 09/19/16 12:04 PM (7 years, 4 months ago) |
|
|
Quote:
Greg said:
Quote:
blindingleaf said:
Quote:
Mycolorado said: Hey htd, if you're going to work with agar, I highly suggest you use petri dishes. They're the proper tool for the job. They're also easier to photograph, even with a shitty phone camera.

nice plates mang!
those reishi plates look like cubensis though. why are they sectoring? is it MS?
That's just what Reishi does in my experience:
 
I guess it's possible we both have MS cultures, but mine came from a seller who said it's an isolate. 
Not sure, could just be reishi mycelial characteristics as Greg suggests or it might be that it's not a monoculture? I've never grown reishi before so don't have any experience. Another member on the gourmet board was kind enough to send me a couple dowels. I'll use one plate to create spawn and work on isolating the other and see what happens...
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
|
hmmm i dunno then. TBH, been a hot minute since I had a reishi plate out, so maybe I'm just remembering wrong
either way, u guys have some nice looking plates
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
|
|
Like leaf said a long ass post will rarely be read my thumb hurts from scrolling down this page haha looking good man
|
|