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Invisibleh0ldthedoor
HODOR
I'm a teapot User Gallery


Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Some plates
    #23607042 - 09/03/16 06:51 AM (7 years, 4 months ago)

Inoculated 0 with a loop using a spore print about a week ago, showed growth slightly smaller than what was photographed today, yesterday. Yesterday, a small portion of the growth was taken and placed on fresh plates (1-3). On number two, a small chunk of agar fell from the blade and that is why there are two spots of growth.

0


1



2


3


There are a couple dozen plates sitting that were inoculated along with 0, although they have yet to show signs of healthy growth. There is one plate that has some funky stuff in the center and white growth around the edges, keeping an eye on it to see if the outside growth becomes viable for a transfer.

Pics are still potato, but hopefully improving..

Any advice, thoughts, feedback is appreciated as always!

Edit: forgot to number photographs


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Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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OfflineKenetic
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Re: Some plates [Re: h0ldthedoor]
    #23607113 - 09/03/16 07:41 AM (7 years, 4 months ago)

Yeah baby


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Offlinehappyherb
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Re: Some plates [Re: Kenetic]
    #23607118 - 09/03/16 07:44 AM (7 years, 4 months ago)

:thumbup:


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Offlinespore-ty
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Registered: 01/21/16
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Re: Some plates [Re: happyherb]
    #23607314 - 09/03/16 09:15 AM (7 years, 4 months ago)

Did you flame your loop prior to use?


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Invisibleh0ldthedoor
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Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Re: Some plates [Re: spore-ty]
    #23607332 - 09/03/16 09:22 AM (7 years, 4 months ago)

Flame to red hot, clean agar to cool and make mark, get some mycelium, place on clean agar, repeated until all three plates had been inoculated. :thumbup:

Oh, and a loop was used for spores to agar on plate 0 and a blade was used for the transfers to plates 1-3.


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


Edited by h0ldthedoor (09/03/16 09:28 AM)


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Offlinespore-ty
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Re: Some plates [Re: h0ldthedoor]
    #23607411 - 09/03/16 09:58 AM (7 years, 4 months ago)

Thanks for that just getting into agar and trying to get a nice over head plan


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Invisibleh0ldthedoor
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Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Re: Some plates [Re: spore-ty]
    #23612093 - 09/04/16 05:31 PM (7 years, 4 months ago)

Taken a few minutes ago, spotting some rhizomorphic growth now

0-a


0-b


1-a


1-b


2-a


2-b


3-a


3-b


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Invisibleh0ldthedoor
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Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Re: Some plates [Re: h0ldthedoor]
    #23615025 - 09/05/16 02:49 PM (7 years, 4 months ago)

Taken a little bit ago. Transfers were made from plates 1-3 to plates 4-6. Plate 0 seems to be doing well after the transfer.

0


1


4


2


5


3


6


Anything look viable for LC?


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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InvisiblemushboyMDiscord
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Re: Some plates [Re: h0ldthedoor]
    #23615075 - 09/05/16 03:10 PM (7 years, 4 months ago)

i REALLY need to find the link but this guy took a colonized plate and put
it in a jar of water and shook it like crazy and used that as his LI.


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Invisibleh0ldthedoor
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Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Re: Some plates [Re: mushboy]
    #23630812 - 09/09/16 10:06 PM (7 years, 4 months ago)

Plate 3 grew out nicely. The growth almost reached the edge of the glass jar. Earlier, the plate (1/2 pint widemouth mason jar) and prepared LC jar were moved to the SAB. The plate was opened and the agar was broken apart (since the container was glass, getting it to plop out was impossible) using a flame sterilized blade. The LC jar was opened and the agar chunks were dropped into the LC jar.

Playing with some other plates while the LC sits. Once it's ready some will go on agar to check for contaminates. If it is contaminated, the LC will be dumped and by that time hopefully another culture will be ready for transfer to LC. The LC looks a little like wildfire when illuminated. Photos to come.


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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OfflinePanarchist
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Registered: 04/02/16
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Re: Some plates [Re: mushboy]
    #23633978 - 09/10/16 09:13 PM (7 years, 4 months ago)

Quote:

mushboy said:
i REALLY need to find the link but this guy took a colonized plate and put
it in a jar of water and shook it like crazy and used that as his LI.





in Munchauzen's video series there's one that covers that: https://www.shroomery.org/forums/showflat.php/Number/21032261#21032261


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InvisibleBoogieman47
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Re: Some plates [Re: Panarchist]
    #23633981 - 09/10/16 09:15 PM (7 years, 4 months ago)

Looking good man


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Offlinejust_curious
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Re: Some plates [Re: Boogieman47]
    #23634109 - 09/10/16 09:59 PM (7 years, 4 months ago)

Start working on isolating. I wish I would've done that when I first started. After a few transfers you will start to see sectoring of different strains. Take a piece if agar from the fastest growing, healthiest looking strains and put to a new agar plate. Repeat the process until you have uniform growth. Also, you may want to switch up your agar recipe every now and then when doing so. Good luck man.


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Offlinemushpunx
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Re: Some plates [Re: h0ldthedoor]
    #23634643 - 09/11/16 02:34 AM (7 years, 4 months ago)

Quote:

h0ldthedoor said:
Flame to red hot, clean agar to cool and make mark, get some mycelium, place on clean agar, repeated until all three plates had been inoculated. :thumbup:

Oh, and a loop was used for spores to agar on plate 0 and a blade was used for the transfers to plates 1-3.





Are you using a loop for agar transfers? I only use mine for innoxulating from spore print.

To make a transfer to a new plate, use a scalpel instead. Take a tiny wedge


--------------------

Amateur Mycologists United
AMU Q&A


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Invisibleh0ldthedoor
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Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Re: Some plates [Re: mushpunx]
    #23635194 - 09/11/16 10:03 AM (7 years, 4 months ago)

Quote:

mushpunx said:
Quote:

h0ldthedoor said:
Oh, and a loop was used for spores to agar on plate 0 and a blade was used for the transfers to plates 1-3.





Are you using a loop for agar transfers? I only use mine for innoxulating from spore print.

To make a transfer to a new plate, use a scalpel instead. Take a tiny wedge




Yes, exactly as you described. Except using an xacto knife in lieu of a scalpel. May check locally for a scalpel soon, though. Wondering if the flaking/corrosion/whatever that is showing up on the blade when flamed will not be as bad with a proper scalpel.

Taking photos of the progress shortly.

Just re-read that, no not using a loop for transfers. Only for spore to agar.


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


Edited by h0ldthedoor (09/11/16 10:06 AM)


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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
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Re: Some plates [Re: h0ldthedoor]
    #23635228 - 09/11/16 10:15 AM (7 years, 4 months ago)

I use my loop for taking pulls from slants. My loop is 20 gauge kanthal vape wire attached to a scalpel handle and it works really well. Haven't used the electric one since I built the flowhood lol.

Also exacto knives are cool but I highly recommend getting a scalpel because you can get huge packs of blades for cheap. I use this scalpel with  these blades.

Anyway, I wouldn't try making an LC with any of those yet. I'd do 2-3 more transfers of the best looking one.


Edited by NumeroEno (09/11/16 11:19 AM)


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Invisibleh0ldthedoor
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Re: Some plates [Re: h0ldthedoor]
    #23635325 - 09/11/16 10:50 AM (7 years, 4 months ago)

Wow that's really economical! Thanks for the tip! :super:

Yeah, the LC is more for practice and learning than anything, especially practicing getting the LC on agar right, when checking for contaminates. Last time working from spore syringe to agar, the plunger stuck and blew more liquid than intended on the plate. Those looked gnarly after a little bit :scat:

0-a


0-b


1


2


lc from 3


4


5


6


Clone attempts from wild fresh cube


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
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Re: Some plates [Re: h0ldthedoor] * 1
    #23635405 - 09/11/16 11:21 AM (7 years, 4 months ago)

They have wild cubes beyond The Wall? No wonder the wildlings are so fuckin crazy lol.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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OfflineMushierage
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Re: Some plates [Re: h0ldthedoor]
    #23635412 - 09/11/16 11:23 AM (7 years, 4 months ago)

I have been following this closely.  Definitely studying hard here because I'm getting 4 ounces of agar tomorrow, which will be immediately used to start a bunch of plates.  Right now I have no cultures or clones, but I do have some B+ and some AA+ spores on the way.  Was hoping to get Mexicube to, but not sure that's going to happen.  Think the trader botched on me.  Can't wait till tomorrow!


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Invisibleh0ldthedoor
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Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Re: Some plates [Re: Mushierage]
    #23656502 - 09/18/16 06:15 PM (7 years, 4 months ago)

Out of room for pics on here, imgur couldn't keep the order right, cbf to re-arrange, the captions on the images are accurate.

  • 0&18 - Inoculated from spores using loop

  • 1,2,6 - Transfered from 0

  • 7,8,9 - Transfered from 1

  • 13&14 - Transfered from 2

  • 10,11,12 - Transfered from 3

  • 15,16,17 - Transfered from 5

  • 19-22 - Transfered from 18


http://imgur.com/a/uw845

A couple of the plates pictured are being moved to refrigeration and testing the LC on agar is next.

Got 6 qt jars + one partial qt of wbs prepped using this tek https://www.shroomery.org/forums/showflat.php/Number/23187393 on-hand now. Oh and one gallon of reclaimed wbs water for things.

Feedback is appreciated!!!

:zoidberg:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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