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Offlineapocalypticshroom
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Why do spores clump?
    #23606902 - 09/03/16 03:48 AM (7 years, 4 months ago)

I could not find this on the forum, there for I'm out here asking the question.

I've noticed that when I store spore syringes in the fridge, that they tend to clump. Of course you shake them up and it becomes un-clumped, but I always wondered why they do this.

A syringe is lying flat in a fridge, so I would expect that they fall to one side (the 'bottom' side), just like leaves of a tree would fall to the ground. But spores don't. They cluster together in some corner of the syringe.
So...

Why do spores cluster?
Does this has anything to do with the power of germination or the genetic make up of the fruitbodys? (like the appearance of the mycelial cords can tell you something about the genetic make up of the shrooms)
Are they stored (too) cold and therefore clustered?

I hope someone can explain this to me


Edited by apocalypticshroom (09/03/16 03:59 AM)


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Re: Why do spores clump [Re: apocalypticshroom] * 1
    #23606910 - 09/03/16 03:59 AM (7 years, 4 months ago)

welcome to the Shroomery!

spores are less dense(id imagine) than water so they are not going to 'sink' to
the bottom. and like a lot of things.. they clump because they are
attracted to each other.

im not quoting science or anything, just common sense that comes to mind :p
or im completely wrong and will soon be ridiculed.. :whatever:


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Re: Why do spores clump [Re: mushboy] * 1
    #23606942 - 09/03/16 05:03 AM (7 years, 4 months ago)

Mushroom spores have a slightly lower pH than that of water. This difference in proton concentration between the two cause the spores to maintain a slightly negative charge. This charge, in turn, causes them to congregate.


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Re: Why do spores clump [Re: mackblack]
    #23606954 - 09/03/16 05:14 AM (7 years, 4 months ago)

:mindexpanding:


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OfflineKenetic
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Re: Why do spores clump [Re: mushboy]
    #23607131 - 09/03/16 07:50 AM (7 years, 4 months ago)

Because they're lonely


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Re: Why do spores clump [Re: mackblack]
    #23607160 - 09/03/16 08:05 AM (7 years, 4 months ago)

Quote:

mackblack said:
Mushroom spores have a slightly lower pH than that of water. This difference in proton concentration between the two cause the spores to maintain a slightly negative charge. This charge, in turn, causes them to congregate.





:like: AWESOME


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Re: Why do spores clump [Re: micolatria]
    #23607179 - 09/03/16 08:15 AM (7 years, 4 months ago)

Spores are sticky.


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Re: Why do spores clump [Re: Pastywhyte]
    #23607245 - 09/03/16 08:47 AM (7 years, 4 months ago)

Primarily :whathesaid:  Negative charge or no, they're straight up sticky.


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Re: Why do spores clump [Re: Inocuole]
    #23607444 - 09/03/16 10:12 AM (7 years, 4 months ago)

Why are they sticky? Is it a protective membrane of sorts?


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Invisibleh0ldthedoor
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Re: Why do spores clump [Re: mackblack]
    #23607461 - 09/03/16 10:19 AM (7 years, 4 months ago)

Quote:

mackblack said:
Mushroom spores have a slightly lower pH than that of water. This difference in proton concentration between the two cause the spores to maintain a slightly negative charge. This charge, in turn, causes them to congregate.




This is correct. Hydrophobic basidiospores may exhibit this characteristic to aid in their dispersal. Also, it has been shown IL-treated basisiospores may improve contrast under observation with a scanning electron microscope without charging effects or crinkling shown in dried, dehydrated, Pt-coated basidiospores. On a semi-related note, when spores are released from a mushroom, the mushroom will create it's own convection current to carry the spores farther away from the fruit body itself, as shown here. Neat stuff.


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OfflinePortabellaFella 1
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Re: Why do spores clump [Re: lovelaughlibs]
    #23607467 - 09/03/16 10:21 AM (7 years, 4 months ago)

Is it because being sticky helps the myc become larger in area to be able to absorb the most nutrients? I suppose if it wasn't sticky and in an area where the myc was growing was to be disturbed and fall away, the organism as a whole would lose that portion of nutritious material. Sooo it evolved to be sticky.


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Re: Why do spores clump [Re: PortabellaFella 1]
    #23607476 - 09/03/16 10:23 AM (7 years, 4 months ago)

What?? I'd imagine spores are sticky because the right 2 spores need to meet to create that single hyphae. If they didn't naturally stick together, they wouldn't reproduce.


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Re: Why do spores clump [Re: PortabellaFella 1]
    #23607483 - 09/03/16 10:26 AM (7 years, 4 months ago)

Quote:

PortabellaFella 1 said:
Sooo it evolved to be sticky.




In gross, oversimplified terms, yes..


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OfflinePortabellaFella 1
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Re: Why do spores clump [Re: h0ldthedoor]
    #23607865 - 09/03/16 12:11 PM (7 years, 4 months ago)

I know he asked why spores stick, I just went further than needed and explained why they stick as a larger organism. I just added to confusion my bad


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InvisibleInocuole
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Re: Why do spores clump [Re: PortabellaFella 1]
    #23608043 - 09/03/16 01:24 PM (7 years, 4 months ago)

You also phrased it as a question, which implies you have no idea.


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OfflinePortabellaFella 1
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Re: Why do spores clump [Re: Inocuole]
    #23608187 - 09/03/16 02:22 PM (7 years, 4 months ago)

Yes it was phrased as a question. I have an idea, the question mark was more me asking if I'm correct.


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OfflineKenetic
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Re: Why do spores clump [Re: Mad Season]
    #23608655 - 09/03/16 04:47 PM (7 years, 4 months ago)

Quote:

Mad Season said:
What?? I'd imagine spores are sticky because the right 2 spores need to meet to create that single hyphae. If they didn't naturally stick together, they wouldn't reproduce.




Man I hate to call you out, just trying to correct some info, but a single spore will produce a single hyphae, which is monokaryotic, and when monokaryotic hyphae from two compatible spores intercept they exchange dna through clamp connections and form dikaryotic hyphae. 

But does any of this matter?  Only if you're trying to create a new strain.


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OfflineMushierage
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Re: Why do spores clump [Re: Kenetic]
    #23608661 - 09/03/16 04:50 PM (7 years, 4 months ago)

Quote:

kenetic said:
Quote:

Mad Season said:
What?? I'd imagine spores are sticky because the right 2 spores need to meet to create that single hyphae. If they didn't naturally stick together, they wouldn't reproduce.




Man I hate to call you out, just trying to correct some info, but a single spore will produce a single hyphae, which is monokaryotic, and when monokaryotic hyphae from two compatible spores intercept they exchange dna through clamp connections and form dikaryotic hyphae. 

But does any of this matter?  Only if you're trying to create a new strain.




Only dikyriotic Myc will reproduce.  Pretty sure that's what he meant.  Js.  And it's not only to create a new strain.  Its so the Myc can produce mushrooms period.  You are misinformed.


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OfflineKenetic
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Re: Why do spores clump [Re: Mushierage]
    #23608672 - 09/03/16 04:54 PM (7 years, 4 months ago)

I'm pretty sure I just ommited stating that dikaryotic myc will produce shrooms and mono will not because he probably knew that already.

My point is that it doesn't take two spores to create hyphae.  It only takes one.


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Re: Why do spores clump [Re: Kenetic] * 1
    #23608770 - 09/03/16 05:28 PM (7 years, 4 months ago)

It takes two spores to create dikaryotic hyphae though, which is the entire point of spores in the first place.  So, you know, proximity is a factor.

Fairly certain not every two spores are compatible either, so clumping is for the best, really.


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OfflineKenetic
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Re: Why do spores clump [Re: Inocuole]
    #23608784 - 09/03/16 05:35 PM (7 years, 4 months ago)

Good point.

O.P. brought up a pretty good question.  It's a cool phenomenon.  I noticed soap bubbles in a dish of water like to clump up too and I always wondered why.

It could be a bunch of reasons like density or magnetic polarity (due to electron/proton levels), stickiness lol or maybe they're just getting their pre-game group huddle on.


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Offlineapocalypticshroom
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Re: Why do spores clump [Re: mackblack]
    #23608793 - 09/03/16 05:38 PM (7 years, 4 months ago)

Thanks for the answer :smile:


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Offlineapocalypticshroom
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Re: Why do spores clump [Re: h0ldthedoor]
    #23608801 - 09/03/16 05:41 PM (7 years, 4 months ago)

Does this mean that all spores of p. cubensis spp. are hydrophobic?


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InvisibleMad Season
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Re: Why do spores clump [Re: Inocuole]
    #23609608 - 09/03/16 10:12 PM (7 years, 4 months ago)

Quote:

Inocuole said:
It takes two spores to create dikaryotic hyphae though, which is the entire point of spores in the first place.  So, you know, proximity is a factor.

Fairly certain not every two spores are compatible either, so clumping is for the best, really.



:whathesaid: it's a 1 in 4 chance you'll get dikaroytic hyphae from 2 spores meeting. If they didn't clump, the chances of mushrooms reproducing would be so fuckin low lol.

But yeah I guess I didn't go into enough detail. English... :wtfsonic:


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Invisiblemorty422
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Re: Why do spores clump [Re: Mad Season]
    #23609654 - 09/03/16 10:31 PM (7 years, 4 months ago)


Lactarius Deceptivus

Looks deceptively sticky!

I'll see myself out... :rofl:


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Re: Why do spores clump [Re: morty422]
    #23619951 - 09/06/16 09:40 PM (7 years, 4 months ago)

Quote:

morty422 said:

Lactarius Deceptivus

Looks deceptively sticky!

I'll see myself out... :rofl:




awesome pic, great :smile:


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